In 1891, Romanowsky successfully used methylene blue solution to detect malarial parasites in blood. Almost incidentally he noticed that white blood cells were also brilliantly colored by the solution. Romanowsky found that best staining was obtained when methylene blue reagent was aged and formed a surface film. For the next decade, many variations of Romanowsky’s original stain were proposed by authorities such as Jenner, Nocht, Leishman, and others. Finally, in 1902 Wright and, in the same year, Giemsa proposed Romanowsky formulations that are still popular throughout the world. Today, stain technology has advanced from Romanowsky’s moldy solution to carefully prepared reagents that have more predictable behavior. Two of the most important Romanowsky reagents are Wright and Giemsa stains. Sigma-Aldrich has chemically defined these stains. Both are precisely formulated, performing optimally and predictably when used either manually or with automated stainers. Other popular products include our Fetal Hemoglobin kit and our Iron and Reticulocyte Stains. The hematology product line includes reagents “For In Vitro Diagnostic Use” for clinical laboratories.
Used to elute adult hemoglobin by the procedure of Oski and Naiman, a modification of the Kleihauer Betke procedure for the demonstration of Fetal Hemoglobin. Included in Sigma procedure 285
Intended for use as a buffer in various Romanowsky type staining procedures, including Wright Stain, Wright Giemsa, Giemsa, May Grunwald, Jenner and Leishman.
Intended for use as a buffer with various Romanowky type staining procedures, specifically those performed on the Hematek. RS2 is a component of Sigma products WGHT, WSHT and WSHT-S.
A special silicone solution in heptane that readily forms a covalent, microscopically thin film on glass, retards clotting of blood or plasma; water repellant. Ideal for glass, ceramics and fiber optics. Used to treat GC injection glass inserts. Ready to use without dilution; reusable if kept free of moisture.
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