Human Protein Function Microarray

Panorama Human p53 Protein Function Microarray is the first fully functional protein array that allows parallel analysis of your protein against multiple p53 proteins. The p53 functional array utilizes a proprietary tagging technology that is based on the presence and biotinylation of a BCCP (biotin-carboxy carrier protein) tag. When the fusion protein is correctly folded and thus functional, the expressed BCCP tag becomes biotinylated. The BCCP-biotin complex then securely tethers the spotted protein to the streptavidin coated slide.

The p53 functional protein microarray contains a human wild-type p53 and 49 SNP variants plus experimental controls to ensure the p53 protein has been spotted and is functional. The p53 proteins are printed in duplicate on a 1 x 3 inch glass slide and sequence verified. Unlike other protein microarrays which use a nitrocellulose coated slide, our p53 proteins are spotted on a streptavidin coated slide which significantly reduces background problems commonly associated with nitrocellulose.

Kit Contents:

• Panorama Human p53 Microarrays - 2 ea
• GADD45 DNA Probe-Cy3
• Anti-p53-Cy5
• Instruction Booklet