Sigma® offers a broad portfolio of three-dimensional cell culture systems including MaxGel™ human Extracellular Matrix (ECM), HydroMatrix™ synthetic peptide, and mouse ECM, to support stem cell and other cell cultures. These products provide three-dimensional environments in which cells are better able to mimic their in vivo counterparts.
A synthetic peptide nanofiber scaffold, HydroMatrix offers the precision and control of a synthesized matrix with the natural three-dimensional architecture of highly crosslinked peptide hydrogel. The HydroMatrix scaffold self-assembles from fluid precursors into a highly cross-linked peptide 3-dimensional hydrogel in response to changes in temperature or ionic strength.
By adjusting the concentration of the HydroMatrix solution, researchers are able to control the flexibility of the 3-D architecture, and tailor the structure to meet their individual needs. HydroMatrix promotes cell growth and migration and has been shown to support the proliferation of many cell types, including neural stem cells, neurons, glia, astrocytes, fibroblasts, and keratinocytes.
Produced in vitro, MaxGel human ECM provides a rich three dimensional environment to promote cellular proliferation. MaxGel ECM contains extracellular matrix components including collagens, laminin, fibronectin, tenascin, elastin, a number of proteoglycans and glycosaminoglycans. The cell-cultured derived ECM effectively reproduces the cooperative interaction of epithelia and mesenchyme during development and in organotypic cell culture of skin. The human MaxGel ECM promotes cell growth and migration and has been shown to support the proliferation of many cell types, including neural stem cells, neurons, glia, astrocytes, fibroblasts, hepatocytes and keratinocytes.