Dulbecco’s Media

Modification of MEM containing increased level of amino acids and vitamins. Supports a wide range of cell types including hybridomas. Many modifications of Eagle′s Medium have been developed since the original formulation appeared in the literature. Among the most widely used of these modifications is Dulbecco′s Modified Eagle′s Medium (DME). DME is a modification of Basal Medium Eagle (BME) that contains a 4-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types.