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Basal Medium Eagle (BME)

Eagle′s Basal Medium (BME), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. There are several ′′basal′′ media described by Eagle that vary slightly from one another. The Tissue Culture Association recommends use of the name ′′Eagle′s Basal Medium′′ to describe only the formula developed to support HeLa cells. This basal medium formula was developed as a result of numerous studies carried out in the late 1950′s to determine the essential nutritional requirements and other factors critical to the growth of cells in culture. Historically, BME has been used in studies conducted to measure the growth response of normal (WI-38) and transformed (mouse and HeLa) cells in monolayer culture. BME, when properly supplemented, has demonstrated wide applicability, for supporting monolayers growth of a wide variety of normal and transformed cell lines. BME is the predecessor of Eagle′s Minimum Essential Medium (MEM) and Dulbecco′s Modified Eagle′s Medium (DME).

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B1522 Basal Medium Eagle With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
B9638 Basal Medium Eagle With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture