Nutrient Mixtures (Ham’s)

Ham′s Nutrient Mixtures were originally developed to support clonal growth of several clones of Chinese hamster ovary (CHO) cells, as well as clones of HeLa and mouse L-cells. Both mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham′s F-10 has been shown to support the growth of human diploid cells, white blood cells for chromosomal analysis, primary explants of rat, rabbit and chicken tissues. Ham′s F-12 has been used for the growth of primary rat hepatocytes and rat prostate epithelial cells. A clonal toxicity assay using CHO cells have also been reported with Ham′s F-12 as the medium of choice. Ham′s F-12 is also available with 25mM HEPES buffer that provides more effective buffering in the optimum pH range of 7.2-7.4.

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51651C Ham’s Nutrient Mixture F12 liquid
N6635 Nutrient Mixture F-10 Ham With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
N6013 Nutrient Mixture F-10 Ham With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
N6908 Nutrient Mixture F-10 Ham With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
N2147 Nutrient Mixture F-10 Ham With 20 mM HEPES, without sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture
N6760 Nutrient Mixture F-12 Ham With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
F6636 Nutrient Mixture F-12 Ham powder, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, Coon′s Modification, suitable for cell culture
N3520 Nutrient Mixture F-12 Ham Kaighn′s Modification, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
N4888 Nutrient Mixture F-12 Ham With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
N6658 Nutrient Mixture F-12 Ham With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
N8641 Nutrient Mixture F-12 Ham HEPES Modification, with 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture