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Iscove’s Modified Dulbecco’s Medium

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum-free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

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I3390 Iscove’s Modified Dulbecco’s Medium liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma
I7633 Iscove’s Modified Dulbecco’s Medium With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
I6529 Iscove’s Modified Dulbecco’s Medium With sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma
51471C Iscove’s Modified Dulbecco’s Medium liquid