Minimum Essential Medium Eagle (MEM)

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium. The formulation has been further modified by optional elimination of calcium to permit growth of cells in suspension culture.

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56419C Minimum Essential Medium with Earle′s Balanced Salts, with 2.0 mM L-glutamine, without sodium bicarbonate, dry powder, suitable for cell culture
56416C Minimum Essential Medium with Earle′s Balanced Salts, with 2.0 mM L-glutamine, with non-essential amino acids, without sodium bicarbonate, dry powder, suitable for cell culture
51412C Minimum Essential Medium with Earle′s Balanced Salts, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
51411C Minimum Essential Medium with Earle′s Balanced Salts, with 2.0 mM L-glutamine, liquid, sterile-filtered, suitable for cell culture
51416C Minimum Essential Medium with Earle′s Balanced Salts, with non-essential amino acids, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
51417C Minimum Essential Medium with Earle′s salts, with 2.0 mM L-glutamine, with 20.0 mM HEPES, with non-essential amino acids, liquid, sterile-filtered, suitable for cell culture
51415C Minimum Essential Medium with Earle′s Balanced Salts, with 25mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
M4526 Minimum Essential Medium Eagle Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture
M8042 Minimum Essential Medium Eagle Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
M0894 Minimum Essential Medium Eagle Alpha Modification, with L-glutamine, without ribonucleosides, deoxyribonucleosides and sodium bicarbonate, powder, suitable for cell culture
M0644 Minimum Essential Medium Eagle Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture
M0769 Minimum Essential Medium Eagle AutoMod, with Earle′s salts, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture
M2645 Minimum Essential Medium Eagle HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
M7278 Minimum Essential Medium Eagle HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
M8028 Minimum Essential Medium Eagle Joklik Modification, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
M0518 Minimum Essential Medium Eagle Joklik Modification, with L-glutamine, without calcium chloride and sodium bicarbonate, suitable for cell culture
M3024 Minimum Essential Medium Eagle with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture
M2414 Minimum Essential Medium Eagle Modified, with Earle′s salts and reduced NaHCO3. without L-glutamine, liquid, sterile-filtered, suitable for cell culture
M8167 Minimum Essential Medium Eagle Spinner Modification, with Earle′s salts and sodium bicarbonate, without calcium chloride and L-glutamine, liquid, sterile-filtered, suitable for cell culture
M0268 Minimum Essential Medium Eagle With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
M0643 Minimum Essential Medium Eagle With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture
M4655 Minimum Essential Medium Eagle With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
M2279 Minimum Essential Medium Eagle With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
M5650 Minimum Essential Medium Eagle With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
M0275 Minimum Essential Medium Eagle 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
M4780 Minimum Essential Medium Eagle With Hanks′ salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
M4642 Minimum Essential Medium Eagle With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
M5775 Minimum Essential Medium Eagle With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
M1018 Minimum Essential Medium Eagle With Hanks′ salts, L-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture
M9288 Minimum Essential Medium Eagle 10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
M0200 Minimum Essential Medium Eagle Alpha Modification, With sodium bicarbonate and L-glutamine, without ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture
M0325 Minimum Essential Medium Eagle With Earle′s salts, non-essential amino acids, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
M0450 Minimum Essential Medium Eagle Alpha Modification, With ribonucleosides, L-glutamine, deoxyribonucleosides and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
56449C Minimum Essential Medium Joklik’s Modified with Earle′s Balanced Salts, with 2.0 mM L-glutamine, without calcium chloride, without sodium bicarbonate, dry powder, suitable for cell culture