Classical Media and Salts

DME F12

During the past decade, researchers have reported the culture of a variety of cell lines in serum-free medium that contained, instead of serum, a supplement of nutrients, growth factors and hormones. For example, Mather and Sato reported the successful cultivation of Leydig cells and Sertoli cells in serum-free medium that contained insulin, transferrin,epidermal growth factor, leutinizing hormone or follicle stimulating hormone, somatomedin and growth hormone. Although the hormones and their concentrations are specific for the type of cell under study, the medium found to be most satisfactory for studies of this type was a 1:1 mixture of Dulbecco's Modified Eagle's Medium (DME) and Ham's F-12 Nutrient Mixture. HEPES buffer is included in the formulation at a final concentration of 15 mM to compensate for the loss of buffering capacity incurred by eliminating serum.

Product # Product Description Application
D 6421 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Liquid
  • With 15 mM HEPES and sodium bicarbonate
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Without L-glutamine, supplement with 0.365 gm/L L-glutamine
  • Endotoxin tested
  • Cell culture tested
  • Sterile-filtered
Liquid form of original 1:1 mixture of DMEM/Ham's F-12, with one important exception.  Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.

Suitable for growth of wide range of cell types in  low serum conditions.

D 8437 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Liquid
  • With 15 mM HEPES, L-glutamine and sodium bicarbonate
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Endotoxin tested
  • Cell culture tested
  • Sterile-filtered
Same as D 6421, except the L-glutamine is added to the medium at time of manufacture.

 

D 8900 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Powder
  • With L-glutamine and 15 mM HEPES
  • w/o sodium bicarbonate, to be supplemented with 1.2 gm/L of sodium bicarbonate
  • Formulated at 15.6 grams of powder per liter of medium
  • Cell culture tested
Powder form of original 1:1 mixture of DME/Ham's F-12. Suitable for growth of a wide range of cell types in low serum conditions.
D 6905 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham Hybi-Max.
  • Powder
  • With L-glutamine and 15 mM HEPES
  • w/o sodium bicarbonate, to be supplemented with 1.2 gm/L of sodium bicarbonate
  • Formulated at 15.6 grams of powder per liter of medium
  • Cell culture tested
Formulation is identical to D 8900.  This formulation has been tested with and is recommended for use with hybridoma cells.
D 6434 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Liquid
  • With 15 mM HEPES and sodium bicarbonate
  • Without phenol red and L-glutamine, supplement with 0.365 gm/L L-glutamine
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Endotoxin tested
  • Sterile-filtered

Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.

Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.

D 2906 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Powder
  • With L-glutamine and 15 mM HEPES
  • Without phenol red and sodium bicarbonate, to be supplemented with 1.2 gm/L of sodium bicarbonate
  • Formulated at 15.6 grams of powder per liter of medium
  • Cell culture tested
Suitable for growth of a wide range of cell types in low serum conditions.

The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.

D 6559 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Liquid
  • With sodium bicarbonate
  • Without phenol red and L-glutamine, supplement with 0.365 gm/L L-glutamine
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Endotoxin tested
  • Cell culture tested
  • Sterile-filtered

Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.

Suitable for growth of a wide range of cell types in low serum conditions. The absence of phenol red makes this a preferred medium for clonal and stem cell culture.

The absence of HEPES allows the investigator to optimize the buffering system.

D 8062 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Liquid
  • With L-glutamine and sodium bicarbonate
  • Without HEPES
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Endotoxin tested
  • Cell culture tested
  • Sterile-filtered
Same as D 8437, except that HEPES has not been added to the formulation.

Allows the investigator to optimize the buffering system.

D 0547 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Powder
  • With L-glutamine and trace elements
  • Without HEPES and sodium bicarbonate, to be supplemented with 1.2 gm/L of sodium bicarbonate
  • Formulated at 12.1 grams of powder per liter of medium
  • Cell culture tested
Powder form of original 1:1 mixture of DME/Ham's F-12, supplemented with trace elements.

Trace element supplementation of the original formula (D 8900) is required for the completely serum free culture of a wide range of cell types.

D 9785 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham Base
  • Powder
  • With 15 mM HEPES
  • Without L-glutamine, L-leucine, L-lysine, L-methionine, calcium chloride, magnesium chloride, magnesium sulfate, phenol red and sodium bicarbonate
  • Supplement with 0.1545 gm/L calcium chloride; 0.365 gm/L L-glutamine; 0.05905 gm/L L-leucine, 0.09125 gm/L L-lysine; 0.0612  gm/L magnesium chloride, 0.04884 gm/L magnesium sulfate, 0.0172 gm/L L-methionine, and 1.2  gm/L sodium bicarbonate
  • Formulated at 14.8 grams of powder per liter of medium
  • Cell culture tested
The most basic formulation offered.  This formulation is used by investigators who want to start with the essential components of (1:1) DME/Ham's F-12, and have the flexibility to optimize the formula for their own application.  This base formulation is particularly suitable for studies of cell protein metabolism with label-tagged amino acids.