| Product # |
Product |
Description |
Application |
| D 5546 |
Dulbecco's Modified Eagle's Medium |
- Liquid
- With 1000 mg/L glucose and sodium bicarbonate
- Without L-glutamine
- With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
- Cell culture tested
- Endotoxin tested
- Sterile-filtered
|
Liquid form of the original Dulbecco's formulation, offered as D 5523, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. |
| D 6046 |
Dulbecco's Modified Eagle's Medium |
- Liquid
- With 1000 mg/L glucose, L-glutamine and sodium bicarbonate
- With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
- Cell culture tested
- Endotoxin tested
- Sterile-filtered
|
Fully supplemented liquid form of the original Dulbecco's formulation, offered as D 5523, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. |
| D 2429 |
Dulbecco's Modified Eagle's Medium (10X) |
- Liquid
- With 1000 mg/L glucose at 1x
- With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
- Without L-glutamine, supplement with 0.584 gm/L L-glutamine, at 1x
- Without sodium bicarbonate, supplement with 3.7 gm/L sodium bicarbonate, at 1x
- Without folic acid, supplement with 0.004 gm/L folic acid, at 1x
- pH 1.8-2.0 at 10x
- Cell culture tested
- Endotoxin tested
- Sterile-filtered
|
Concentrated liquid version of the original low glucose Dulbecco's formulation, with the pyridoxine substitution (see D 5546).
Because of its low solubility, folic acid is added after the medium is diluted to 1x and the pH has been adjusted.
|
| D 5523 |
Dulbecco's Modified Eagle's Medium |
- Powder
- With L-glutamine and 1000 mg/L glucose
- Without sodium bicarbonate, supplement with 3.7 gm/L sodium bicarbonate
- Formulated at 10.0 grams of powder per liter of medium
- Cell culture tested
|
Provides investigator with the option to modify the buffering system. Also, this is Dulbecco's original low glucose formulation. |
| D 5921 |
Dulbecco's Modified Eagle's Medium Modified |
- Liquid
- With 1000 mg/L glucose, and sodium bicarbonate
- Without phenol red and L-glutamine, supplement with 0.584 gm/L L-glutamine
- With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
- Cell culture tested
- Endotoxin tested
- Sterile-filtered
|
Liquid form of the original Dulbecco's formulation, offered as D 5523, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.
See D 2902 for note on phenol red
|
| D 2902 |
Dulbecco's Modified Eagle's Medium Modified |
- Powder
- With L-glutamine and 1000 mg/L glucose
- Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
- Without phenol red
- Formulated at 10.0 grams of powder per liter of medium
- Cell culture tested
|
Investigators who work with stem cells or cell clones frequently prefer media without phenol red. |
| D 5671 |
Dulbecco's Modified Eagle's Medium |
- Liquid
- With 4500 mg/L glucose and sodium bicarbonate
- Without L-glutamine, supplement with 0.584 gm/L L-glutamine
- Cell culture tested
- Hybridoma tested
- Endotoxin tested
- Sterile-filtered
|
Complete liquid form of the high glucose modification of Dulbecco's original formulation.
Fully tested.
|
| D 5796 |
Dulbecco's Modified Eagle's Medium |
- Liquid
- With 4500 mg/L glucose, L-glutamine and sodium bicarbonate
- With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
- Cell culture tested
- Endotoxin tested
- Sterile-filtered
|
Complete liquid form of the high glucose version of Dulbecco's original formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. |
| D 2554 |
Dulbecco's Modified Eagle's Medium (10X) |
- Liquid
- With 4500 mg/L glucose at 1x
- With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCL)
- Without L-glutamine, supplement with 0.584 gm/L L-glutamine, at 1x
- Without sodium bicarbonate, supplement with 3.7 gm/L sodium bicarbonate, at 1x
- Without folic acid, supplement with 0.004 gm/L folic acid, at 1x
- pH 1.8-2.0 at 10x
- Cell culture tested
- Endotoxin tested
- Sterile-filtered
|
Concentrated liquid version of the modified high glucose Dulbecco's formulation, with the pyridoxine substitution (see D 5796).
Because of its low solubility, folic acid is added after the medium is diluted to 1x and the pH has been adjusted.
|
| D 5648 |
Dulbecco's Modified Eagle's Medium |
- Powder
- With L-glutamine and 4500 mg/L glucose
- Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
- Formulated at 13.4 grams of powder per liter of medium
- Cell culture tested
- Related to DME, Hybridoma tested (D 6655)
|
Modified version of D 5523. Many cell lines have been found to require a higher glucose level than was provided in the original formulation. |
| D 6655 |
Dulbecco's Modified Eagle's Medium Hybri-Max. |
- Powder
- With L-glutamine and 4500 mg/L glucose
- Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
- Formulated at 13.4 grams of powder per liter of medium
- Cell culture tested
- Hybridoma tested
- Endotoxin tested
|
Same as D 5648, except that it is tested with and recommended for use with hybridoma cells. It is the powder version of D 5671. |
| D 6546 |
Dulbecco's Modified Eagle's Medium |
- Liquid
- With 4500 mg/L glucose, 110 mg/L sodium pyruvate and sodium bicarbonate
- Without L-glutamine, supplement with 0.584 gm/L L-glutamine
- With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
- Cell culture tested
- Endotoxin tested
- Sterile-filtered
|
Pyruvate supplemented, complete, liquid form of the high glucose version of Dulbecco's original formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.
Glutamine to be added by the investigator
|
| D 6429 |
Dulbecco's Modified Eagle's Medium |
- Liquid
- With 4500 mg/L glucose, L-glutamine and sodium pyruvate
- With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
- Cell culture tested
- Endotoxin tested
- Sterile-filtered
|
Pyruvate supplemented, complete, liquid form of the high glucose version of Dulbecco's original formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. |
| D 7777 |
Dulbecco's Modified Eagle's Medium |
- Powder
- With L-glutamine, 4500 mg/L glucose and 110 mg/L sodium pyruvate
- Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
- Formulated at 13.5 grams of powder per liter of medium
- Cell culture tested
- Related to DME, Hybridoma tested (D 6780)
- Cell culture tested
|
Same as D 5648, except that it is supplemented with the additional energy source pyruvate. |
| D 6780 |
Dulbecco's Modified Eagle's Medium Hybri-Max. |
- Powder
- With L-glutamine, 4500 mg/L glucose and 110 mg/L sodium pyruvate
- Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
- Formulated at 13.5 grams of powder per liter of medium
- Hybridoma tested
- Endotoxin tested
|
Same as D 7777, except that it is tested with and recommended for use with hybridoma cells. |
| D 6171 |
Dulbecco's Modified Eagle's Medium HEPES Modified |
- Liquid
- With 4500 mg/L glucose, 25 mM HEPES and sodium bicarbonate
- Without L-glutamine, supplement with 0.584 gm/L L-glutamine
- With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
- Cell culture tested
- Endotoxin tested
- Sterile-filtered
|
Pyruvate supplemented, complete, liquid form of the high glucose version of Dulbecco's original formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.
See D 1152 for discussion of HEPES.
|
| D 1152 |
Dulbecco's Modified Eagle's Medium HEPES Modification |
- Powder
- With L-glutamine and 4500 mg/L glucose and 25 mM HEPES
- Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
- Formulated at 17.4 grams of powder per liter of medium
- Cell culture tested
|
Same as D 5648, except that it is supplemented with HEPES. HEPES supplementation increases the buffering capacity of the medium. Recommended for cells that require a higher pH (7.3-7.6) for optimal growth. |
| D 0422 |
Dulbecco's Modified Eagle's Medium |
- Liquid
- With 4500 mg/L glucose, and sodium bicarbonate
- Without L-methionine, L-cystine and L-glutamine, supplement with 0.584 gm/L L-glutamine
- Cell culture tested
- Endotoxin tested
- Sterile-filtered
|
Deficient liquid form of the high glucose version of Dulbecco's original formulation. Key amino acids have been left out to allow researchers to set up metabolic studies with tagged amino acids. |
| D 3656 |
Dulbecco's Modified Eagle's Medium |
- Powder
- With L-glutamine and 4500 mg/L glucose
- Without sodium phosphate and sodium bicarbonate; supplement with 3.7 gm/L of sodium bicarbonate, 0.109 gm/L sodium phosphate mono-basic (anhydrous)
- Formulated at 13.3 grams of powder per liter of medium
- Cell culture tested
|
Provides the investigator with the option and flexibility to control, modify or develop the medium buffering system. |
| D 5280 |
Dulbecco's Modified Eagle's Medium Auto-Mod™ |
- Powder
- Modified for autoclaving
- With 1000 mg/L glucose
- Without L-glutamine and sodium bicarbonate; supplement with 0.584 gm/L of L-glutamine; 3.7 gm/L of sodium bicarbonate
- Formulated at 9.6 grams of powder per liter of medium
- Cell culture tested
|
Same as D 5523, except that L-glutamine and sodium bicarbonate have been removed so that this medium can be formulated and autoclaved. After autoclaving, the L-glutamine and sodium bicarbonate can be added. |
| D 5030 |
Dulbecco's Modified Eagle's Medium Base |
- Powder
- Without L-glutamine, glucose, phenol red, sodium pyruvate and sodium bicarbonate
- Supplement with 0.584 gm/L of L-glutamine; 1.0 gm/L glucose; 3.7 gm/L of sodium bicarbonate
- Formulated at 8.3 grams of powder per liter of medium
- Cell culture tested
|
The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application. |
