Classical Media and Salts

DMEM

Many modifications of Eagle's Medium have been developed since the original formulation appeared in the literature. Among the most widely used of these modifications is Dulbecco's Modified Eagle's medium (DMEM). DMEM is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DMEM formula contains 1000 mg/L of glucose and was first reported for culturing embryonic mouse cells. A further alteration with 4500 mg/L glucose has proved to be optimal for cultivation of certain cell types.

Product # Product Description Application
D 5546 Dulbecco's Modified Eagle's Medium
  • Liquid
  • With 1000 mg/L glucose and sodium bicarbonate
  • Without L-glutamine
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered 
Liquid form of the original Dulbecco's formulation, offered as D 5523, with one key exception.  Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.
D 6046 Dulbecco's Modified Eagle's Medium
  • Liquid
  • With 1000 mg/L glucose, L-glutamine and sodium bicarbonate
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Fully supplemented liquid form of the original Dulbecco's formulation, offered as D 5523, with one key exception.  Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.
D 2429 Dulbecco's Modified Eagle's Medium (10X)
  • Liquid
  • With 1000 mg/L glucose at 1x
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Without L-glutamine, supplement with 0.584 gm/L L-glutamine, at 1x
  • Without sodium bicarbonate, supplement with 3.7 gm/L sodium bicarbonate, at 1x
  • Without folic acid, supplement with 0.004 gm/L folic acid, at 1x
  • pH 1.8-2.0 at 10x
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered

Concentrated liquid version of the original low glucose Dulbecco's formulation, with the pyridoxine substitution (see D 5546).

Because of its low solubility, folic acid is added after the medium is diluted to 1x and the pH has been adjusted.

D 5523 Dulbecco's Modified Eagle's Medium
  • Powder
  • With L-glutamine and 1000 mg/L glucose
  • Without sodium bicarbonate, supplement with 3.7 gm/L  sodium bicarbonate
  • Formulated at 10.0 grams of powder per liter of medium
  • Cell culture tested
Provides investigator with the option to modify the buffering system. Also, this is Dulbecco's original low glucose formulation.
D 5921 Dulbecco's Modified Eagle's Medium Modified
  • Liquid
  • With 1000 mg/L glucose, and sodium bicarbonate
  • Without phenol red and L-glutamine, supplement with 0.584 gm/L L-glutamine
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Liquid form of the original Dulbecco's formulation, offered as D 5523, with one key exception.  Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.

See D 2902 for note on phenol red

D 2902 Dulbecco's Modified Eagle's Medium Modified
  • Powder
  • With L-glutamine and 1000 mg/L glucose
  • Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
  • Without phenol red
  • Formulated at 10.0 grams of powder per liter of medium
  • Cell culture tested
Investigators who work with stem cells or cell clones frequently prefer media without phenol red.
D 5671 Dulbecco's Modified Eagle's Medium
  • Liquid
  • With 4500 mg/L glucose and sodium bicarbonate
  • Without L-glutamine, supplement with 0.584 gm/L L-glutamine
  • Cell culture tested
  • Hybridoma tested
  • Endotoxin tested
  • Sterile-filtered
Complete liquid form of the high glucose modification of Dulbecco's original formulation.

Fully tested.

D 5796 Dulbecco's Modified Eagle's Medium
  • Liquid
  • With 4500 mg/L glucose, L-glutamine and sodium bicarbonate
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Complete liquid form of the high glucose version of Dulbecco's original formulation, with one key exception.   Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.
D 2554 Dulbecco's Modified Eagle's Medium (10X)
  • Liquid
  • With 4500 mg/L glucose at 1x
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCL)
  • Without L-glutamine, supplement with 0.584 gm/L L-glutamine, at 1x
  • Without sodium bicarbonate, supplement with 3.7 gm/L sodium bicarbonate, at 1x
  • Without folic acid, supplement with 0.004 gm/L folic acid, at 1x
  • pH 1.8-2.0 at 10x
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Concentrated liquid version of the modified high glucose Dulbecco's formulation, with the pyridoxine substitution (see D 5796).

Because of its low solubility, folic acid is added after the medium is diluted to 1x and the pH has been adjusted.

 

D 5648 Dulbecco's Modified Eagle's Medium
  • Powder
  • With L-glutamine and 4500 mg/L glucose
  • Without sodium bicarbonate, supplement with  3.7 gm/L of  sodium bicarbonate
  • Formulated at 13.4 grams of powder per liter of medium
  • Cell culture tested
  • Related to DME, Hybridoma tested (D 6655)
Modified version of D 5523. Many cell lines have been found to require a higher glucose level than was provided in the original formulation.
D 6655 Dulbecco's Modified Eagle's Medium Hybri-Max.
  • Powder
  • With L-glutamine and 4500 mg/L glucose
  • Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
  • Formulated at 13.4 grams of powder per liter of medium
  • Cell culture tested
  • Hybridoma tested
  • Endotoxin tested
Same as D 5648, except that it is tested with and recommended for use with hybridoma cells.  It is the powder version of  D 5671.  
D 6546 Dulbecco's Modified Eagle's Medium
  • Liquid
  • With 4500 mg/L glucose, 110 mg/L sodium pyruvate and sodium bicarbonate
  • Without L-glutamine, supplement with 0.584 gm/L  L-glutamine
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Pyruvate supplemented, complete, liquid form of the high glucose version of Dulbecco's original formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.

Glutamine to be added by the investigator

D 6429 Dulbecco's Modified Eagle's Medium
  • Liquid
  • With 4500 mg/L glucose, L-glutamine and sodium pyruvate
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Pyruvate supplemented, complete, liquid form of the high glucose version of Dulbecco's original formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.
D 7777 Dulbecco's Modified Eagle's Medium
  • Powder
  • With L-glutamine,  4500 mg/L glucose and 110 mg/L sodium pyruvate
  • Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
  • Formulated at 13.5 grams of powder per liter of medium
  • Cell culture tested
  • Related to DME, Hybridoma tested (D 6780)
  • Cell culture tested
Same as D 5648, except that it is supplemented with  the additional energy source pyruvate.  
D 6780 Dulbecco's Modified Eagle's Medium Hybri-Max.
  • Powder
  • With L-glutamine,  4500 mg/L glucose and 110 mg/L sodium pyruvate
  • Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
  • Formulated at 13.5 grams of powder per liter of medium
  • Hybridoma tested
  • Endotoxin tested
Same as D 7777, except that it is tested with and recommended for use with hybridoma cells.
D 6171 Dulbecco's Modified Eagle's Medium HEPES Modified
  • Liquid
  • With 4500 mg/L glucose, 25 mM HEPES and sodium bicarbonate
  • Without L-glutamine, supplement with 0.584 gm/L  L-glutamine
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Pyruvate supplemented, complete, liquid form of the high glucose version of Dulbecco's original formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.

See D 1152 for discussion of HEPES.

D 1152 Dulbecco's Modified Eagle's Medium HEPES Modification
  • Powder
  • With L-glutamine and 4500 mg/L glucose and 25 mM HEPES
  • Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
  • Formulated at 17.4 grams of powder per liter of medium
  • Cell culture tested
Same as D 5648, except that it is supplemented with HEPES.  HEPES supplementation increases the buffering capacity of the medium.  Recommended for cells that require a higher pH (7.3-7.6) for optimal growth.
D 0422 Dulbecco's Modified Eagle's Medium
  • Liquid
  • With 4500 mg/L glucose, and sodium bicarbonate
  • Without L-methionine, L-cystine and L-glutamine, supplement with  0.584 gm/L  L-glutamine
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Deficient liquid form of the high glucose version of Dulbecco's original formulation.  Key amino acids have been left out to allow researchers to set up metabolic studies with tagged amino acids.
D 3656 Dulbecco's Modified Eagle's Medium
  • Powder
  • With L-glutamine and 4500 mg/L glucose
  • Without sodium phosphate and sodium bicarbonate; supplement with 3.7 gm/L of sodium bicarbonate, 0.109 gm/L sodium phosphate mono-basic (anhydrous)
  • Formulated at 13.3 grams of powder per liter of medium
  • Cell culture tested
Provides the investigator with the option and flexibility to control, modify or develop the medium buffering system.
D 5280 Dulbecco's Modified Eagle's Medium Auto-Mod™
  • Powder
  • Modified for autoclaving
  • With 1000 mg/L glucose
  • Without L-glutamine and sodium bicarbonate; supplement with 0.584 gm/L of L-glutamine; 3.7 gm/L of sodium bicarbonate
  • Formulated at 9.6 grams of powder per liter of medium
  • Cell culture tested
Same as  D 5523, except that L-glutamine and sodium bicarbonate have been removed so that this medium can be formulated and autoclaved. After autoclaving, the L-glutamine and sodium bicarbonate can be added.
D 5030 Dulbecco's Modified Eagle's Medium Base
  • Powder
  • Without L-glutamine, glucose, phenol red, sodium pyruvate and sodium bicarbonate
  • Supplement with 0.584 gm/L of L-glutamine; 1.0 gm/L glucose; 3.7 gm/L of sodium bicarbonate
  • Formulated at 8.3 grams of powder per liter of medium
  • Cell culture tested
The most basic formulation offered.  This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application.