Cell Culture

Dulbecco's Media

 Dulbecco's Phosphate Buffered Saline (D-PBS)

The use of balanced salt solution (BSS) in cell and tissue culture is generally attributed to early workers in the field. In 1885, Sydney Ringer developed a solution of inorganic salts designed to maintain contractility of mammalian heart tissue. A less specific salt solution was designed by Tyrode for use in work with primary mammalian cells. Tyrode's salt solution became the accepted fluid for diluting protein components of media of natural origin. Since that time, many other balanced salt solutions have been developed for use in cell and tissue culture. The current role of balanced salt solution in cell culture is multi-faceted and can be divided into four principle functions.

  • As an irrigating, transporting and diluting fluid while maintaining intra- and extra-cellular osmotic balance;
  • Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism;
  • Combined with a carbohydrate, such as glucose, provides the principle energy source for cell metabolism; and
  • Provides buffering system to maintain the medium within the physiological pH range (7.2-7.6)
Product No. Product Name Description Application
D8662 Dulbecco's Phosphate Buffered Saline
  • Liquid
  • With calcium chloride and magnesium chloride
  • Cell culture tested
  • Sterile-filtered
  • Endotoxin teste
Known as D-PBS, this is a general use, isotonic saline solution for washing cells and tissues.
D1283 Dulbecco's Phosphate Buffered Saline (10X)
  • Liquid
  • With calcium chloride and magnesium chloride
  • Cell culture tested
  • Sterile-filtered
  • pH 4.2-4.6 at 10X
  • Endotoxin tested
Concentrated form of D 8662.
D8537 Dulbecco's Phosphate Buffered Saline Modified
  • Liquid
  • w/o calcium chloride and magnesium chloride
  • Cell culture tested
  • Sterile-filtered
  • Endotoxin tested
Calcium and magnesium promote cell adhesion. Use this formulation of D-PBS when tissue or cell dissociation or release is the objective.
D1408 Dulbecco's Phosphate Buffered Saline (10X)
  • Liquid
  • Cell culture tested
  • Sterile-filtered
  • pH 6.6-7.0 at 10X
  • Endotoxin tested
Concentrated form of D 8537.
D5652 Dulbecco's Phosphate Buffered Saline Modified
  • Powder
  • w/o calcium chloride and magnesium chloride
  • Cell culture tested
  • Formulated at 9.6 grams of powder per liter of medium
  • Related products: Dulbecco's Phosphate Buffered Saline Hybri-Max (D7030)
Powder form of D 8537Calcium and magnesium promote cell adhesion.

Use this formulation of D-PBS when tissue or cell dissociation or release is the objective.
D7030 Dulbecco's Phosphate Buffered Saline Modified Hybri-Max.
  • Powder
  • w/o calcium chloride and magnesium chloride
  • Hybridoma tested
  • Formulated at 9.6 grams of powder per liter of medium
  • Endotoxin tested
Same as D 5652 except it is tested for use with hybridoma cells.
D5773 Dulbecco's Phosphate Buffered Saline
  • Powder
  • w/o calcium chloride
  • Cell culture tested
  • Formulated at 9.7 grams of powder per liter of medium
Calcium  promotes cell adhesion.Use this formulation of D-PBS when tissue or cell dissociation or release is the objective.

Also, suitable for studies that involve calcium metabolism.
D4031 Dulbecco's Phosphate Buffered Saline Modified
  • Liquid
  • With 36 mg/L sodium pyruvate, 50 mg/L streptomycin sulfate, 100 mg/L kanamycin monosulfate, 1000 mg/L glucose, calcium chloride, and magnesium chloride.
  • Sterile-filtered
  • Endotoxin tested
Fully supplemented isotonic solution.  Contains energy sources and antibiotics. 

Frequently used with primary cells where risk of contamination may be high.
D6650 Dulbecco's Phosphate Buffered Saline Modified
  • Powder
  • With 36 mg/L sodium pyruvate, 50 mg/L streptomycin sulfate and 1.000 mg/L glucose.
  • w/o calcium chloride and penicillin G
  • Cell culture tested
  • Penicillin G supplied separately with each package for addition at a final concentration of 1 million units/L. Calcium chloride di-hydrate is supplied separately with each package for final concentration of 133 mg/L.
Isotonic saline solution that contains energy sources and antibiotics.

Provides the option to formulate with or without penicillin G or calcium.

Frequently used with primary cells where risk of contamination may be high and where tissue dissociation is an objective.


Dulbecco's Modified Eagle's Medium (DME)

Many modifications of Eagle's Medium have been developed since the original formulation appeared in the literature. Among the most widely used of these modifications is Dulbecco's Modified Eagle's medium (DME). DME is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula contains 1000 mg/L of glucose and was first reported for culturing embryonic mouse cells. A further alteration with 4500 mg/L glucose has proved to be optimal for cultivation of certain cell types.
 

Product No. Product Description Application
D5546 Dulbecco's Modified Eagle's Medium
  • Liquid
  • With 1000 mg/L glucose and sodium bicarbonate
  • Without L-glutamine
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered 
Liquid form of the original Dulbecco's formulation, offered as D5523, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.
D6046 Dulbecco's Modified Eagle's Medium
  • Liquid
  • With 1000 mg/L glucose, L-glutamine and sodium bicarbonate
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Fully supplemented liquid form of the original Dulbecco's formulation, offered as D5523, with one key exception.  Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.
D2429 Dulbecco's Modified Eagle's Medium (10X)
  • Liquid
  • With 1000 mg/L glucose at 1x
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Without L-glutamine, supplement with 0.584 gm/L L-glutamine, at 1x
  • Without sodium bicarbonate, supplement with 3.7 gm/L sodium bicarbonate, at 1x
  • Without folic acid, supplement with 0.004 gm/L folic acid, at 1x
  • pH 1.8-2.0 at 10x
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Concentrated liquid version of the original low glucose Dulbecco's formulation, with the pyridoxine substitution (see D5546).

Because of its low solubility, folic acid is added after the medium is diluted to 1x and the pH has been adjusted.
D5523 Dulbecco's Modified Eagle's Medium
  • Powder
  • With L-glutamine and 1000 mg/L glucose
  • Without sodium bicarbonate, supplement with 3.7 gm/L  sodium bicarbonate
  • Formulated at 10.0 grams of powder per liter of medium
  • Cell culture tested
Provides investigator with the option to modify the buffering system. Also, this is Dulbecco's original low glucose formulation.
D5921 Dulbecco's Modified Eagle's Medium Modified
  • Liquid
  • With 1000 mg/L glucose, and sodium bicarbonate
  • Without phenol red and L-glutamine, supplement with 0.584 gm/L L-glutamine
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Liquid form of the original Dulbecco's formulation, offered as D5523, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.See D2902 for note on phenol red
D2902 Dulbecco's Modified Eagle's Medium Modified
  • Powder
  • With L-glutamine and 1000 mg/L glucose
  • Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
  • Without phenol red
  • Formulated at 10.0 grams of powder per liter of medium
  • Cell culture tested
Investigators who work with stem cells or cell clones frequently prefer media without phenol red.
D5671 Dulbecco's Modified Eagle's Medium
  • Liquid
  • With 4500 mg/L glucose and sodium bicarbonate
  • Without L-glutamine, supplement with 0.584 gm/L L-glutamine
  • Cell culture tested
  • Hybridoma tested
  • Endotoxin tested
  • Sterile-filtered
Complete liquid form of the high glucose modification of Dulbecco's original formulation.Fully tested.
D5796 Dulbecco's Modified Eagle's Medium
  • Liquid
  • With 4500 mg/L glucose, L-glutamine and sodium bicarbonate
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Complete liquid form of the high glucose version of Dulbecco's original formulation, with one key exception.   Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.
D2554 Dulbecco's Modified Eagle's Medium (10X)
  • Liquid
  • With 4500 mg/L glucose at 1x
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCL)
  • Without L-glutamine, supplement with 0.584 gm/L L-glutamine, at 1x
  • Without sodium bicarbonate, supplement with 3.7 gm/L sodium bicarbonate, at 1x
  • Without folic acid, supplement with 0.004 gm/L folic acid, at 1x
  • pH 1.8-2.0 at 10x
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Concentrated liquid version of the modified high glucose Dulbecco's formulation, with the pyridoxine substitution (see D5796).Because of its low solubility, folic acid is added after the medium is diluted to 1x and the pH has been adjusted.
D5648 Dulbecco's Modified Eagle's Medium
  • Powder
  • With L-glutamine and 4500 mg/L glucose
  • Without sodium bicarbonate, supplement with  3.7 gm/L of  sodium bicarbonate
  • Formulated at 13.4 grams of powder per liter of medium
  • Cell culture tested
  • Related to DME, Hybridoma tested (D 6655)
Modified version of D5523. Many cell lines have been found to require a higher glucose level than was provided in the original formulation.
D6655 Dulbecco's Modified Eagle's Medium Hybri-Max.
  • Powder
  • With L-glutamine and 4500 mg/L glucose
  • Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
  • Formulated at 13.4 grams of powder per liter of medium
  • Cell culture tested
  • Hybridoma tested
  • Endotoxin tested
Same as D5648, except that it is tested with and recommended for use with hybridoma cells. It is the powder version of  D 5671.  
D6546 Dulbecco's Modified Eagle's Medium
  • Liquid
  • With 4500 mg/L glucose, 110 mg/L sodium pyruvate and sodium bicarbonate
  • Without L-glutamine, supplement with 0.584 gm/L  L-glutamine
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Pyruvate supplemented, complete, liquid form of the high glucose version of Dulbecco's original formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.Glutamine to be added by the investigator
D6429 Dulbecco's Modified Eagle's Medium
  • Liquid
  • With 4500 mg/L glucose, L-glutamine and sodium pyruvate
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Pyruvate supplemented, complete, liquid form of the high glucose version of Dulbecco's original formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.
D7777 Dulbecco's Modified Eagle's Medium
  • Powder
  • With L-glutamine,  4500 mg/L glucose and 110 mg/L sodium pyruvate
  • Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
  • Formulated at 13.5 grams of powder per liter of medium
  • Cell culture tested
  • Related to DME, Hybridoma tested (D 6780)
  • Cell culture tested
Same as D5648, except that it is supplemented with  the additional energy source pyruvate.
D6780 Dulbecco's Modified Eagle's Medium Hybri-Max.
  • Powder
  • With L-glutamine,  4500 mg/L glucose and 110 mg/L sodium pyruvate
  • Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
  • Formulated at 13.5 grams of powder per liter of medium
  • Hybridoma tested
  • Endotoxin tested
Same as D7777, except that it is tested with and recommended for use with hybridoma cells.
D6171 Dulbecco's Modified Eagle's Medium HEPES Modified
  • Liquid
  • With 4500 mg/L glucose, 25 mM HEPES and sodium bicarbonate
  • Without L-glutamine, supplement with 0.584 gm/L  L-glutamine
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Pyruvate supplemented, complete, liquid form of the high glucose version of Dulbecco's original formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.See D1152 for discussion of HEPES.
D1152 Dulbecco's Modified Eagle's Medium HEPES Modification
  • Powder
  • With L-glutamine and 4500 mg/L glucose and 25 mM HEPES
  • Without sodium bicarbonate, supplement with 3.7 gm/L of sodium bicarbonate
  • Formulated at 17.4 grams of powder per liter of medium
  • Cell culture tested
Same as D5648, except that it is supplemented with HEPES.  HEPES supplementation increases the buffering capacity of the medium. Recommended for cells that require a higher pH (7.3-7.6) for optimal growth.
D0422 Dulbecco's Modified Eagle's Medium
  • Liquid
  • With 4500 mg/L glucose, and sodium bicarbonate
  • Without L-methionine, L-cystine and L-glutamine, supplement with  0.584 gm/L  L-glutamine
  • Cell culture tested
  • Endotoxin tested
  • Sterile-filtered
Deficient liquid form of the high glucose version of Dulbecco's original formulation. Key amino acids have been left out to allow researchers to set up metabolic studies with tagged amino acids.
D3656 Dulbecco's Modified Eagle's Medium
  • Powder
  • With L-glutamine and 4500 mg/L glucose
  • Without sodium phosphate and sodium bicarbonate; supplement with 3.7 gm/L of sodium bicarbonate, 0.109 gm/L sodium phosphate mono-basic (anhydrous)
  • Formulated at 13.3 grams of powder per liter of medium
  • Cell culture tested
Provides the investigator with the option and flexibility to control, modify or develop the medium buffering system.
D5280 Dulbecco's Modified Eagle's Medium Auto-Mod™
  • Powder
  • Modified for autoclaving
  • With 1000 mg/L glucose
  • Without L-glutamine and sodium bicarbonate; supplement with 0.584 gm/L of L-glutamine; 3.7 gm/L of sodium bicarbonate
  • Formulated at 9.6 grams of powder per liter of medium
  • Cell culture tested
Same as  D5523, except that L-glutamine and sodium bicarbonate have been removed so that this medium can be formulated and autoclaved. After autoclaving, the L-glutamine and sodium bicarbonate can be added.
D5030 Dulbecco's Modified Eagle's Medium Base
  • Powder
  • Without L-glutamine, glucose, phenol red, sodium pyruvate and sodium bicarbonate
  • Supplement with 0.584 gm/L of L-glutamine; 1.0 gm/L glucose; 3.7 gm/L of sodium bicarbonate
  • Formulated at 8.3 grams of powder per liter of medium
  • Cell culture tested
The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application.


 DME/Nutrient Mixture F-12 Hams (DME/F-12)

During the past decade, researchers have reported the culture of a variety of cell lines in serum-free medium that contained, instead of serum, a supplement of nutrients, growth factors and hormones. For example, Mather and Sato reported the successful cultivation of Leydig cells and Sertoli cells in serum-free medium that contained insulin, transferrin,epidermal growth factor, leutinizing hormone or follicle stimulating hormone, somatomedin and growth hormone. Although the hormones and their concentrations are specific for the type of cell under study, the medium found to be most satisfactory for studies of this type was a 1:1 mixture of Dulbecco's Modified Eagle's Medium (DME) and Ham's F-12 Nutrient Mixture. HEPES buffer is included in the formulation at a final concentration of 15 mM to compensate for the loss of buffering capacity incurred by eliminating serum.
 

Product No. Product Description Application
D6421 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Liquid
  • With 15 mM HEPES and sodium bicarbonate
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Without L-glutamine, supplement with 0.365 gm/L L-glutamine
  • Endotoxin tested
  • Cell culture tested
  • Sterile-filtered
Liquid form of original 1:1 mixture of DMEM/Ham's F-12, with one important exception.  Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.Suitable for growth of wide range of cell types in  low serum conditions.
D8437 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Liquid
  • With 15 mM HEPES, L-glutamine and sodium bicarbonate
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Endotoxin tested
  • Cell culture tested
  • Sterile-filtered
Same as D6421, except the L-glutamine is added to the medium at time of manufacture.
D8900 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Powder
  • With L-glutamine and 15 mM HEPES
  • w/o sodium bicarbonate, to be supplemented with 1.2 gm/L of sodium bicarbonate
  • Formulated at 15.6 grams of powder per liter of medium
  • Cell culture tested
Powder form of original 1:1 mixture of DME/Ham's F-12. Suitable for growth of a wide range of cell types in low serum conditions.
D6905 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham Hybi-Max.
  • Powder
  • With L-glutamine and 15 mM HEPES
  • w/o sodium bicarbonate, to be supplemented with 1.2 gm/L of sodium bicarbonate
  • Formulated at 15.6 grams of powder per liter of medium
  • Cell culture tested
Formulation is identical to D8900.  This formulation has been tested with and is recommended for use with hybridoma cells.
D6434 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Liquid
  • With 15 mM HEPES and sodium bicarbonate
  • Without phenol red and L-glutamine, supplement with 0.365 gm/L L-glutamine
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Endotoxin tested
  • Sterile-filtered
Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.

Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.
D2906 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Powder
  • With L-glutamine and 15 mM HEPES
  • Without phenol red and sodium bicarbonate, to be supplemented with 1.2 gm/L of sodium bicarbonate
  • Formulated at 15.6 grams of powder per liter of medium
  • Cell culture tested
Suitable for growth of a wide range of cell types in low serum conditions.The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.
D6559 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Liquid
  • With sodium bicarbonate
  • Without phenol red and L-glutamine, supplement with 0.365 gm/L L-glutamine
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Endotoxin tested
  • Cell culture tested
  • Sterile-filtered
Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.

Suitable for growth of a wide range of cell types in low serum conditions. The absence of phenol red makes this a preferred medium for clonal and stem cell culture.

The absence of HEPES allows the investigator to optimize the buffering system.
D8062 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Liquid
  • With L-glutamine and sodium bicarbonate
  • Without HEPES
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Endotoxin tested
  • Cell culture tested
  • Sterile-filtered
Same as D8437, except that HEPES has not been added to the formulation.Allows the investigator to optimize the buffering system.
D0547 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Powder
  • With L-glutamine and trace elements
  • Without HEPES and sodium bicarbonate, to be supplemented with 1.2 gm/L of sodium bicarbonate
  • Formulated at 12.1 grams of powder per liter of medium
  • Cell culture tested
Powder form of original 1:1 mixture of DME/Ham's F-12, supplemented with trace elements.Trace element supplementation of the original formula (D8900) is required for the completely serum free culture of a wide range of cell types.
D9785 Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham Base
  • Powder
  • With 15 mM HEPES
  • Without L-glutamine, L-leucine, L-lysine, L-methionine, calcium chloride, magnesium chloride, magnesium sulfate, phenol red and sodium bicarbonate
  • Supplement with 0.1545 gm/L calcium chloride; 0.365 gm/L L-glutamine; 0.05905 gm/L L-leucine, 0.09125 gm/L L-lysine; 0.0612  gm/L magnesium chloride, 0.04884 gm/L magnesium sulfate, 0.0172 gm/L L-methionine, and 1.2  gm/L sodium bicarbonate
  • Formulated at 14.8 grams of powder per liter of medium
  • Cell culture tested
The most basic formulation offered.  This formulation is used by investigators who want to start with the essential components of (1:1) DME/Ham's F-12, and have the flexibility to optimize the formula for their own application.  This base formulation is particularly suitable for studies of cell protein metabolism with label-tagged amino acids.