Cell Culture

Ham's Nutrient Mixtures

Ham's Nutrient Mixtures were originally developed to support growth of several clones of Chinese hamster ovary (CHO) cells, as well as clones of HeLa and mouse L-cells.  Both mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured.

  • Ham's F-10 has been shown to support the growth of human diploid cells, white blood cells for chromosomal analysis, primary explants of rat, rabbit and chicken tissues. 
  • Ham's F-12  has been used for the growth of primary rat hepatocytes and rat prostate epithelial cells.  A clonal toxicity assay using CHO cells has also been reported with Ham's F-12 as the medium of choice.  Ham's F-12 is also available with 25 mM HEPES buffer that provides more effective buffering in the optimum pH range of 7.2-7.4
  • Coon's modification of Ham's F-12 was developed for culturing hybrid cells that were produced by viral fusion.  The modification consists of doubling the amino acids and pyruvate and including ascorbic acid.  The salt concentrations have also been altered.  The formula contains 0.863 mg/L zinc sulfate, which may render it unsuitable for culturing mouse L-cells.
  • Kaighn's modification of Ham's F-12 and Coon's F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs).  This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.
Product No. Product Name Description Application
N6013 Nutrient Mixture F-10 Ham
(Ham's F-10)
  • Liquid
  • With sodium bicarbonate
  • Without L-glutamine; supplement with 0.146 gm/L L-glutamine
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Supports the growth of human diploid cells, white blood cells for chromosomal analysis, primary explants of rat, rabbit and chicken tissues. Add L-glutamine to formulation at time of use.
N6908 Nutrient Mixture F-10 Ham 
(Ham's F-10)
  • Liquid
  • With L-glutamine and sodium bicarbonate
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Complete, fully supplemented version of N 6013.
N6635 Nutrient Mixture F-10 Ham
(Ham's F-10)
  • Powder
  • With L-glutamine
  • Without sodium bicarbonate; supplement with 1.2 gm/L sodium bicarbonate;
  • Formulated at 9.8 grams of powder per liter of medium;
  • Cell culture tested
Powder version of N 6908. Sodium bicarbonate is added at the time that the liquid medium is formulated.  
N2147 Nutrient Mixture F-10 Ham
(Ham's F-10)
  • Liquid
  • With 20 mM HEPES
  • Without L-glutamine and sodium bicarbonate: supplement with 0.146 gm/L L-glutamine;
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Contains HEPES and no bicarbonate. HEPES increases the buffer strength of the medium and raises the maximum buffering range. Recommended for cells that grow better at a pH above 7.2.
N1387 Nutrient Mixture F-10 Ham HEPES Modification
(Ham's F-10)
  • Powder
  • With L-glutamine and 25 mM HEPES;
  • Without sodium bicarbonate; supplement with 1.2 gm/L sodium bicarbonate
  • Formulated at 15.2 grams of powder per liter of medium;
  • Cell culture tested
Contains both HEPES and bicarbonate. HEPES increases the buffer strength of the medium and raises the maximum buffering range. Recommended for cells that grow better at a pH above 7.2.
N4888 Nutrient Mixture F-12
(Ham's F-12)
  • Liquid
  • With sodium bicarbonate
  • Without L-glutamine; supplement with 0.146 gm/L L-glutamine
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Developed for growth of primary rat hepatocytes and rat prostate epithelial cells.  Also used in a clonal toxicity assay using CHO cells. Sodium bicarbonate is added at the time of formulation.
N6658 Nutrient Mixture F-12
(Ham's F-12)
  • Liquid
  • With L-glutamine and sodium bicarbonate
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Complete, fully supplemented version of N 4888. Developed for growth of primary rat hepatocytes and rat prostate epithelial cells.  Also used in a clonal toxicity assay using CHO cells.
N6760 Nutrient Mixture F-12 Ham
(Ham's F-12)
  • Powder
  • With L-glutamine
  • Without sodium bicarbonate; supplement with 1.176 gm/L sodium bicarbonate;
  • Formulated at 10.7 grams of powder per liter of medium;
  • Cell culture tested
Powder version of N 6658. Developed for growth of primary rat hepatocytes and rat prostate epithelial cells.  Also used in a clonal toxicity assay using CHO cells.
N8641 Nutrient Mixture F-12 Ham HEPES Modification
(Ham's F-12)
  • Liquid
  • With sodium bicarbonate and 25 mM HEPES;
  • Without L-glutamine; supplement with 0.146 gm/L L-glutamine
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Modified buffering system based upon HEPES. HEPES increases the buffer strength of the medium and raises the maximum buffering range. Recommended for cells that grow better at a pH above 7.2.
N4388 Nutrient Mixture F-12 Ham HEPES Modification
(Ham's F-12)
  • Powder
  • With L-glutamine and 25 mM HEPES;
  • Without sodium bicarbonate; supplement with 1.176 gm/L sodium bicarbonate;
  • Formulated at 16.2 grams of powder per liter of medium;
  • Cell culture tested
Powder version of N8641. Supplement with sodium bicarbonate at time of formulation.
F6636 Nutrient Mixture F-12 Ham F-12, Coon's Modification 
(Ham's F-12 Coon's)
  • Powder
  • With L-glutamine and 0.863 mg/L zinc sulfate;
  • Without sodium bicarbonate; supplement with 2.676 gm/L sodium bicarbonate;
  • Formulated at 11.6 grams of powder per liter of medium;
  • Cell culture tested
Coon's modification of Ham's F-12 was developed for culturing hybrid cells that were produced by viral fusion.  The modification consists of doubling the amino acids and pyruvate and including ascorbic acid.  The salt concentrations have also been altered.  The formula contains 0.863 mg/L zinc sulfate, which may render it unsuitable for culturing mouse L-cells.
N3520 Nutrient Mixture F-12 Ham Kaighn's Modification 
(Ham's F-12K)
  • Powder
  • With L-glutamine
  • Without sodium bicarbonate; supplement with 2.5 gm/L sodium bicarbonate;
  • Formulated at 11.3 grams of powder per liter of medium;
  • Cell culture tested
Kaighn's modification of Ham's F-12 and Coon's F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs).  This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.