Cell Culture

Medium 199

Many early tissue culture media were predominantly formulated from animal products and/or tissue extracts.  In 1950, Morgan and his coworkers reported their efforts to produce a totally defined nutritional source for cell cultures.  Their experiments, conducted with various combinations of vitamins, amino acids, and other factors revealed that growth of explanted tissue could be measured in what has become known as Medium 199.  However, it was found that long-term cultivation of cells required addition of a serum supplement to the culture fluid.  When properly supplemented, Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells.  It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.
 

Product No. Product Name Description Application
M4530 Medium 199
  • Liquid
  • With Earle's salts, L-glutamine, and sodium bicarbonate;
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Complete liquid version of the original M199 formulation published by Morgan.

One of the early media formulations used for virus production.
M2154 Medium 199
  • Liquid
  • With Earle's salts and sodium bicarbonate;
  • Without L-glutamine; supplement with 0.1 gm/L L-glutamine
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Same as M4530, except that L-glutamine must be added by the investigator.
M0650 Medium 199 (10x)
  • Liquid
  • With Earle's salts
  • Without L-glutamine and sodium bicarbonate; supplement with 0.1 gm/L L-glutamine, 2.2 gm/L sodium bicarbonate
  • pH 3.1-4.1 at 10x
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
This is the concentrated version (10x) of M4530.

L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.
M5017 Medium 199
  • Powder
  • With Earle's salts and L-glutamine
  • Without  sodium bicarbonate: supplement with 2.2 gm/L sodium bicarbonate;
  • Formulated at 9.9 grams of powder per liter of medium;
  • Cell culture tested
Powder version of M4530.

Sodium bicarbonate is added at the time that the liquid medium is formulated.
M3769 Medium 199 Modified
  • Powder
  • With Earle's salts
  • Without phenol red, L-glutamine and sodium bicarbonate: supplement with 0.1 gm/L L-glutamine, 2.2 gm/L sodium bicarbonate;
  • Formulated at 9.8 grams of powder per liter of medium;
  • Cell culture tested
Related to M4530, but without phenol red.

Sodium bicarbonate and L-glutamine are added at the time that the liquid medium is formulated.

Phenol red has been shown to interfere with the growth of some cells at low or cloning densities.

Use this version of Medium 199 when working with stem cells or cells at low densities.
M7528 Medium 199 HEPES Modified
  • Liquid
  • With Earle's salts, sodium bicarbonate and 25 mM HEPES;
  • Without L-glutamine; supplement with 0.1 gm/L L-glutamine
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Same as M2154, and M4530, except the buffer system is modified.

HEPES increases the buffer strength of the medium and raises the maximum buffering range.

Recommended for cells that grow better at a pH above 7.2.
M2520 Medium 199 HEPES Modified
  • Powder
  • With Earle's salts, L-glutamine and 25 mM HEPES;
  • Without  sodium bicarbonate: supplement with 2.2 gm/L sodium bicarbonate;
  • Formulated at 15.1 grams of powder per liter of medium;
  • Cell culture tested
Powder version of M7528.

HEPES increases the buffer strength of the medium and raises the maximum buffering range.

Recommended for cells that grow better at a pH above 7.2
M7653 Medium 199
  • Liquid
  • With Hanks' salts and sodium bicarbonate;
  • Without L-glutamine; supplement with 0.1 gm/L L-glutamine
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Modification of the original Medium 199. Earle's salts are replaced with Hanks' salts.  Hanks' salts use a lower concentration of bicarbonate and different concentrations and salt forms of the essential inorganic ions.
M9163 Medium 199 (10x)
  • Liquid
  • With Hanks' salts;
  • Without L-glutamine and sodium bicarbonate; supplement with 0.1 gm/L L-glutamine, 0.35 gm/L sodium bicarbonate
  • pH 4.2-4.4 at 10x
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
This is the concentrated version (10x) of M7653.

L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.
M0393 Medium 199
  • Powder
  • With Hanks' salts and L-glutamine
  • Without  sodium bicarbonate: supplement with 0.35 gm/L sodium bicarbonate;
  • Formulated at 11.0 grams of powder per liter of medium;
  • Cell culture tested
Powder version of M7653. Sodium bicarbonate is added at the time that the liquid medium is formulated.
M3274 Medium 199 Modified
  • Powder
  • With Hanks' salts
  • Without phenol red, L-glutamine and sodium bicarbonate: supplement with 0.1 gm/L L-glutamine, 0.35 gm/L sodium bicarbonate;
  • Formulated at 10.9 grams of powder per liter of medium;
  • Cell culture tested
Related to M0393, but without phenol red. Sodium bicarbonate and L-glutamine are added at the time that the liquid medium is formulated.

Phenol red has been shown to interfere with the growth of some cells at low or cloning densities.

Use this version of Medium 199 when working with stem cells or cells at low densities.