Cell Culture

RPMI Media

RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.


Product No. Product Name Description Application
R0883 RPMI-1640 Medium
  • Liquid
  • With sodium bicarbonate
  • Without L-glutamine; supplement with 0.3 gm/L L-glutamine
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Same as R8758, except that L-glutamine must be added by the investigator.
R8758 RPMI-1640 Medium
  • Liquid
  • With sodium bicarbonate and L-glutamine
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Complete liquid version of the original RPMI-1640 formulation published by Moore et. al. in 1967.

Developed for growth of human normal and neoplastic leukocytes (peripheral blood lymphocytes).  Generally, supplemented with serum.
R1145 RPMI-1640 Medium (10x)
  • Liquid
  • Without L-glutamine, folic acid and sodium bicarbonate
  • Supplement with 0.3 gm/L L-glutamine, 0.001gm/L folic acid and 2.0 gm/L sodium bicarbonate at 1x
  • pH 1.8-2.2 at 10x
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
This is the concentrated version (10x) of R8758.

The L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.

R6504 RPMI-1640 Medium
  • Powder
  • With L-glutamine
  • Without sodium bicarbonate; supplement with 2.0 gm/L sodium bicarbonate.
  • Formulated at 10.4 grams of powder per liter of medium.
  • Cell culture tested
Related to R5382, RPMI-1640 Medium Hybri-Max.
R7509 RPMI-1640 Medium Modified
  • Liquid
  • With sodium bicarbonate
  • Without phenol red and  L-glutamine;  supplement with 0.3 gm/L L-glutamine.
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Phenol red has been shown to interfere with the growth of some cells at clonal densities.

Use this medium when working with stem cells or when growing cells at low densities.

Also recommended for in vitro diagnostics use.
R5886 RPMI-1640 Medium HEPES Modification
  • Liquid
  • With 25 mM HEPES and sodium bicarbonate
  • Without; L-glutamine; supplement with 0.3 gm/L L-glutamine.
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Same as R8758, except that HEPES buffer has been added.

HEPES increases the buffer strength of the medium and raises the maximum buffering range.

Recommended for cells that grow better at a pH above 7.2.
R4130 RPMI-1640 Medium HEPES Modification
  • Powder
  • With L-glutamine and 25 mM HEPES
  • Without sodium bicarbonate; supplement with 2.0 gm/L sodium bicarbonate.
  • Formulated at 16.4 grams of powder per liter of medium.
  • Cell culture tested
Same as R8758, except that HEPES buffer has been added.

HEPES increases the buffer strength of the medium and raises the maximum buffering range.

Recommended for cells that grow better at a pH above 7.2.
R7638 RPMI-1640 Medium Dutch Modification
  • Liquid
  • With 1 gm/L sodium bicarbonate and 20 mM HEPES
  • Without L-glutamine; supplement with 0.3 gm/L L-glutamine.
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Same as R8758, except that the concentration of sodium bicarbonate has been cut in half and HEPES buffer has been added.

HEPES increases the buffer strength of the medium and raises the maximum buffering range.

Recommended for cells that grow better at a pH above 7.2.
R7388 RPMI-1640 Medium Modified
  • Liquid
  • With 20 mM HEPES and L-glutamine
  • Without sodium bicarbonate; supplement with 2.0 gm/L sodium bicarbonate.
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Same as R8758, except that HEPES buffer has been added.

HEPES increases the buffer strength of the medium and raises the maximum buffering range.

Recommended for cells that grow better at a pH above 7.2.
R8755 RPMI-1640 Medium Modified
  • Powder
  • With L-glutamine
  • Without phenol red and sodium bicarbonate; supplement with 2.0 gm/L sodium bicarbonate.
  • Formulated at 10.4 grams of powder per liter of medium.
  • Cell culture tested
Phenol red has been shown to interfere with the growth of some cells at clonal densities.

Use this medium when working with stem cells or when growing cells at low densities.

Also recommended for in vitro diagnostics use.
R7755 RPMI-1640 Medium Auto-Mod™
  • Powder
  • Without L-glutamine and sodium bicarbonate; supplement with 0.3 gm/L of L-glutamine and 2.0 gm/L sodium bicarbonate.
  • Formulated at 10.3 grams of powder per liter of medium.
  • Cell culture tested
Modified for autoclaving
R1383 RPMI-1640 Medium
  • Powder
  • With L-glutamine
  • Without glucose and sodium bicarbonate; supplement with 2.0 gm/L sodium bicarbonate.
  • Formulated at 8.4 grams of powder per liter of medium.
  • Cell culture tested
Without glucose, allows investigator to add own energy source.
R7513 RPMI-1640 Medium Modified
  • Liquid
  • Without sodium bicarbonate
  • Without L-methionine, L-cystine, and L-glutamine
  • Supplement with 0.0652 gm/L L-cystine-2HCL, 0.015 gm/L L-methionine, and 0.3 gm/L of L-glutamine
  • Sterile-filtered
  • Endotoxin tested
  • Cell culture tested
Formulated without sulfur containing amino acids.

Recommended for metabolism studies using radio-labeled sulfur containing amino acids.

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