RPMI Media

RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.

Product #	Product Name	Description	Application
R 0883	RPMI-1640 Medium	Liquid With sodium bicarbonate Without L-glutamine; supplement with 0.3 gm/L L-glutamine Sterile-filtered Endotoxin tested Cell culture tested	Same as R 8758, except that L-glutamine must be added by the investigator.
R 8758	RPMI-1640 Medium	Liquid With sodium bicarbonate and L-glutamine Sterile-filtered Endotoxin tested Cell culture tested	Complete liquid version of the original RPMI-1640 formulation published by Moore et. al. in 1967. Developed for growth of human normal and neoplastic leukocytes (peripheral blood lymphocytes). Generally, supplemented with serum.
R 1145	RPMI-1640 Medium (10x)	Liquid Without L-glutamine, folic acid and sodium bicarbonate Supplement with 0.3 gm/L L-glutamine, 0.001gm/L folic acid and 2.0 gm/L sodium bicarbonate at 1x pH 1.8-2.2 at 10x Sterile-filtered Endotoxin tested Cell culture tested	This is the concentrated version (10x) of R 8758. The L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.
R 6504	RPMI-1640 Medium	Powder With L-glutamine Without sodium bicarbonate; supplement with 2.0 gm/L sodium bicarbonate. Formulated at 10.4 grams of powder per liter of medium. Cell culture tested	Related to R 5382, RPMI-1640 Medium Hybri-Max.
R 7509	RPMI-1640 Medium Modified	Liquid With sodium bicarbonate Without phenol red and L-glutamine; supplement with 0.3 gm/L L-glutamine. Sterile-filtered Endotoxin tested Cell culture tested	Phenol red has been shown to interfere with the growth of some cells at clonal densities. Use this medium when working with stem cells or when growing cells at low densities. Also recommended for in vitro diagnostics use.
R 5886	RPMI-1640 Medium HEPES Modification	Liquid With 25 mM HEPES and sodium bicarbonate Without; L-glutamine; supplement with 0.3 gm/L L-glutamine. Sterile-filtered Endotoxin tested Cell culture tested	Same as R 8758, except that HEPES buffer has been added. HEPES increases the buffer strength of the medium and raises the maximum buffering range. Recommended for cells that grow better at a pH above 7.2.
R 4130	RPMI-1640 Medium HEPES Modification	Powder With L-glutamine and 25 mM HEPES Without sodium bicarbonate; supplement with 2.0 gm/L sodium bicarbonate. Formulated at 16.4 grams of powder per liter of medium. Cell culture tested	Same as R 8758, except that HEPES buffer has been added. HEPES increases the buffer strength of the medium and raises the maximum buffering range. Recommended for cells that grow better at a pH above 7.2.
R 7638	RPMI-1640 Medium Dutch Modification	Liquid With 1 gm/L sodium bicarbonate and 20 mM HEPES Without L-glutamine; supplement with 0.3 gm/L L-glutamine. Sterile-filtered Endotoxin tested Cell culture tested	Same as R 8758, except that the concentration of sodium bicarbonate has been cut in half and HEPES buffer has been added. HEPES increases the buffer strength of the medium and raises the maximum buffering range. Recommended for cells that grow better at a pH above 7.2.
R 7388	RPMI-1640 Medium Modified	Liquid With 20 mM HEPES and L-glutamine Without sodium bicarbonate; supplement with 2.0 gm/L sodium bicarbonate. Sterile-filtered Endotoxin tested Cell culture tested	Same as R 8758, except that HEPES buffer has been added. HEPES increases the buffer strength of the medium and raises the maximum buffering range. Recommended for cells that grow better at a pH above 7.2.
R 8755	RPMI-1640 Medium Modified	Powder With L-glutamine Without phenol red and sodium bicarbonate; supplement with 2.0 gm/L sodium bicarbonate. Formulated at 10.4 grams of powder per liter of medium. Cell culture tested	Phenol red has been shown to interfere with the growth of some cells at clonal densities. Use this medium when working with stem cells or when growing cells at low densities. Also recommended for in vitro diagnostics use.
R 7755	RPMI-1640 Medium Auto-Mod™	Powder Without L-glutamine and sodium bicarbonate; supplement with 0.3 gm/L of L-glutamine and 2.0 gm/L sodium bicarbonate. Formulated at 10.3 grams of powder per liter of medium. Cell culture tested	Modified for autoclaving
R 1383	RPMI-1640 Medium	Powder With L-glutamine Without glucose and sodium bicarbonate; supplement with 2.0 gm/L sodium bicarbonate. Formulated at 8.4 grams of powder per liter of medium. Cell culture tested	Without glucose, allows investigator to add own energy source.
R 7513	RPMI-1640 Medium Modified	Liquid Without sodium bicarbonate Without L-methionine, L-cystine, and L-glutamine Supplement with 0.652 gm/L L-cystine-2HCL, 0.015 gm/L L-methionine, and 0.3 gm/L of L-glutamine Sterile-filtered Endotoxin tested Cell culture tested	Formulated without sulfur containing amino acids. Recommended for metabolism studies using radio-labeled sulfur containing amino acids.
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