Chapters	Title
	1	Introduction
	2	Design and Equipment for the Cell Culture Laboratory
	3	Safety Aspects of Cell Culture
	4	Sourcing of Cell Lines
	5	Cell Types & Culture Characteristics
	6	The Cell Environment (including types of culture medium)
	7	Cryopreservation and Storage of Cells
	8	Good Cell Banking Practices
	9	Quality Control Considerations in Cell Culture
	10	Authentication of Cell Lines
	11	Alternative Culture Systems
	12	Basic Techniques - The “Do’s and Don’ts” of Cell Culture
	Protocols	Title
	1	Aseptic Technique and Good Cell Culture Practice
	2	Resuscitation of Frozen Cell Lines
	3	Subculture of Adherent Cell Lines
	4	Subculture of Semi-Adherent Cell Lines
	5	Subculture of Suspension Cell Lines
	6	Cell Quantification
	7	Cryopreservation of Cell Lines
	8	Testing for Bacteria and Fungi in Cell Cultures
	9	Testing for Mycoplasma by Culture Isolation
	10	Testing for Mycoplasma by Indirect DNA Stain
	Glossary of Terms Who to Contact Request a physical copy of ECACC Handbook.

Abbreviations
ACDP	Advisory Committee on Dangerous Pathogens
BSA	Bovine Serum albumin
BSE	Bovine spongiform encephalopathy
BVDV	Bovine viral diarrhoea virus
FBS	Fetal bovine serum
EC	European Community
ECACC	European Collection of Cell Cultures
FDA	Food and Drug Administration
FCS	Fetal Calf Serum
HEPA	High Efficiency Particulate Air
MCB	Master Cell Bank
NAMAS	National Accreditation of Measurement and Sampling
NCTC	National Collection of Type Cultures
PPE	Personal Protective Equipment
USDA	United States Department of Agriculture
WCB	Working Cell Banks

Chemical Symbols
Ca2+	calcium ion
CO2	carbon dioxide
DMSO	dimethyl sulphoxide
Mg2+	magnesium ion
O2	oxygen
PBS	phosphate buffered saline
TGM	Thioglycollate medium

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ECACC Contact Information:
ECACC
CAMR
Salisbury
Wiltshire, SP4 0JG, UK
Tel +44 (0) 1980 612512
Fax +44 (0) 1980 611315
eMail ecacc@camr.org.uk
Web www.ecacc.org.uk

The ECACC Cell Line Collections contain over 35,000 Cell Lines, delivered to any destination in the world.

• 40 Species
• 50 Tissue Types
• 350 HLA Defined
• 450 Hybridomas
• 500 Human Control
• 750 Human Genetic Disorders

 

Contact us for your FREE Cell Line and Services Catalogue, available as printed hardcopy and CD-ROM.

Sigma-Aldrich and ECACC would like to thank Dr. Alison Stacey, Cambridge, UK and Labcaire, Clevedon, UK for their help in compiling this laboratory handbook.

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