Fundamental Techniques in Cell Culture Laboratory Handbook – Glossary

	Chapters	Title
	1	Introduction
	2	Design and Equipment for the Cell Culture Laboratory
	3	Safety Aspects of Cell Culture
	4	Sourcing of Cell Lines
	5	Cell Types & Culture Characteristics
	6	The Cell Environment (including types of culture medium)
	7	Cryopreservation and Storage of Cells
	8	Good Cell Banking Practices
	9	Quality Control Considerations in Cell Culture
	10	Authentication of Cell Lines
	11	Alternative Culture Systems
	12	Basic Techniques - The “Do’s and Don’ts” of Cell Culture
	Protocols	Title
	1	Aseptic Technique and Good Cell Culture Practice
	2	Resuscitation of Frozen Cell Lines
	3	Subculture of Adherent Cell Lines
	4	Subculture of Semi-Adherent Cell Lines
	5	Subculture of Suspension Cell Lines
	6	Cell Quantification
	7	Cryopreservation of Cell Lines
	8	Testing for Bacteria and Fungi in Cell Cultures
	9	Testing for Mycoplasma by Culture Isolation
	10	Testing for Mycoplasma by Indirect DNA Stain
	Glossary of Terms Who to Contact Request a physical copy of ECACC Handbook.

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ECACC Contact Information:
ECACC
CAMR
Salisbury
Wiltshire, SP4 0JG, UK
Tel +44 (0) 1980 612512
Fax +44 (0) 1980 611315
eMail ecacc@camr.org.uk
Web www.ecacc.org.uk

The ECACC Cell Line Collections contain over 35,000 Cell Lines, delivered to any destination in the world.

• 40 Species
• 50 Tissue Types
• 350 HLA Defined
• 450 Hybridomas
• 500 Human Control
• 750 Human Genetic Disorders

Contact us for your FREE Cell Line and Services Catalogue, available as printed hardcopy and CD-ROM.

Sigma-Aldrich and ECACC would like to thank Dr. Alison Stacey, Cambridge, UK and Labcaire, Clevedon, UK for their help in compiling this laboratory handbook.

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