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Cell Culture
Techniques Table 4
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| Technology |
Suspension |
Attached |
Max Vol
(ml) |
Max S/A
(cm2) |
Max cells
(susp) |
Max cells
(att) |
Advantages |
Disadvantages |
| T Flask |
Yes |
Yes |
150 |
225 |
1.5x108 |
~107 |
Cheap, disposable, no cleaning / sterilization required |
Small scale. Multiples required for larger batches. |
| Triple Flask |
(Yes) |
Yes |
150 |
525 |
1.5x108 |
3x107 |
Cheap, disposable, no cleaning / sterilization required |
Difficult to harvest attached cells. Multiples required for larger batches. |
| Cell Factory |
N/A |
Yes |
8000 |
25280 |
N/A |
1.5x1010 |
Disposable – Single batch manufacture |
Require additional equipment (vessels etc which may require cleaning). Difficult to harvest cells. |
| Roller Bottles |
Yes |
Yes |
1000 |
1700 |
1x109 |
1x108 |
Cheap, disposable, no cleaning / sterilization required. Versatile. Automated systems available. |
Require "decks" to turn. Multiples required for larger batches. Automation very costly. |
| Expanded Roller Bottles |
N/A |
Yes |
(1000) |
3400 |
N/A |
2x108 |
As above |
As above (no advantage for suspension cells). |
| Shake Flasks |
Yes |
N/A |
1000 |
N/A |
1 x 109 |
N/A |
Some disposables available. |
Suspension Only*. Glass vessels to be cleaned & sterilized. Requires Shaker incubator. |
| Spinner Flasks |
Yes |
N/A |
1000 |
N/A |
1 x 109 |
N/A |
Some semi-disposables available |
Suspension Only* Glass vessels to be cleaned & sterilized. Requires Stirrer-base + incubator. |
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