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Cell Culture

Ames' Medium Formulation

Component A1420
g/L
Inorganic Salts
Calcium Chloride 0.1275
Magnesium Sulfate 0.1488
Potassium Chloride 0.231
Potassium Phosphate Monobasic (anhydrous) 0.068
Sodium Chloride 7.01
Amino Acids
L-Alanine 0.0024
L-Arginine • HCl 0.00421
L-Asparagine (anhydrous) 0.00084
L-Aspartic Acid 0.00012
L-Cystine • 2HCl 0.000065
L-Glutamine 0.073
L-Glutamic Acid (sodium) 0.001183
Glycine 0.00045
L-Histidine • HCl • H2O 0.002513
L-Isoleucine 0.00058
L-Leucine 0.00144
L-Lysine • HCl 0.003648
L-Methionine 0.00039
L-Phenylalanine 0.00132
L-Proline 0.00007
L-Serine 0.00252
L-Taurine 0.00075
L-Threonine 0.00333
L-Tryptophan 0.00049
L-Tyrosine • 2Na • 2H2O 0.00211
L-Valine 0.00176
Vitamins
Ascorbic Acid • Na 0.01796
D-Biotin 0.0001
Choline Chloride 0.0007
Folic Acid 0.0001
myo-Inositol 0.0272
Niacinamide 0.0001
D-Pantothenic Acid (hemicalcium) 0.0001
Pyridoxal • HCl 0.0001
Riboflavin 0.00001
Thiamine • HCl 0.0001
Other
Cytidine 0.00073
D-Glucose 1.081
Hypoxanthine 0.00082
Pyruvic Acid (sodium) 0.01333
Thymidine 0.00024
Uridine 0.00073
Add
Sodium Bicarbonate 1.9
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