Cell Culture Nutrient Mixture F-10 Ham Formulation

Component N2147 [1x] g/L N6013 g/L Inorganic Salts Calcium Chloride 0.0333 0.0333 Cupric Sulfate • 5H2O 0.0000025 0.0000025 Ferrous Sulfate • 7H2O 0.000834 0.000834 Magnesium Sulfate (anhydrous) 0.07464 0.07464 Potassium Chloride 0.285 0.285 Potassium Phosphate Monobasic (anhydrous) 0.083 0.083 Sodium Bicarbonate — 1.2 Sodium Chloride 7.4 7.4 Sodium Phosphate Dibasic (anhydrous) 0.1537 0.1537 Zinc Sulfate • 7H2O 0.0000288 0.0000288 Amino Acids L-Alanine 0.009 0.009 L-Arginine • HCl 0.211 0.211 L-Asparagine • H2O 0.01501 0.01501 L-Aspartic Acid 0.0133 0.0133 L-Cysteine • HCl • H2O 0.035 0.035 L-Glutamic Acid 0.0147 0.0147 Glycine 0.00751 0.00751 L-Histidine • 3HCl • H2O 0.021 0.021 L-Isoleucine 0.0026 0.0026 L-Leucine 0.0131 0.0131 L-Lysine • HCl 0.0293 0.0293 L-Methionine 0.00448 0.00448 L-Phenylalanine 0.00496 0.00496 L-Proline 0.0115 0.0115 L-Serine 0.0105 0.0105 L-Threonine 0.00357 0.00357 L-Tryptophan 0.0006 0.0006 L-Tyrosine • 2Na • 2H2O 0.00261 0.00261 L-Valine 0.0035 0.0035 Vitamins D-Biotin 0.000024 0.000024 Choline Chloride 0.000698 0.000698 Folic Acid 0.00132 0.00132 myo-Inositol 0.000541 0.000541 Niacinamide 0.000615 0.000615 D-Pantothenic Acid (hemicalcium) 0.000715 0.000715 Pyridoxine • HCl 0.000206 0.000206 Riboflavin 0.000376 0.000376 Thiamine • HCl 0.001 0.001 Vitamin B12 0.00136 0.00136 Other D-Glucose 1.1 1.1 HEPES 4.77 — Hypoxanthine 0.00408 0.00408 Phenol Red (sodium) 0.0013 0.0013 Pyruvic Acid (sodium) 0.11 0.11 Thioctic Acid 0.00021 0.00021 Thymidine 0.00073 0.00073 Add L-Glutamine 0.146 0.146

Component N6635 g/L N6908 [1x] g/L Inorganic Salts Calcium Chloride 0.0333 0.0333 Cupric Sulfate • 5H2O 0.0000025 0.000025 Ferrous Sulfate • 7H2O 0.000834 0.000834 Magnesium Sulfate (anhydrous) 0.07464 0.07464 Potassium Chloride 0.285 0.285 Potassium Phosphate Monobasic (anhydrous) 0.083 0.083 Sodium Bicarbonate — 1.2 Sodium Chloride 7.4 7.4 Sodium Phosphate Dibasic (anhydrous) 0.1537 0.1537 Zinc Sulfate • 7H2O 0.0000288 0.0000288 Amino Acids L-Alanine 0.009 0.009 L-Arginine • HCl 0.211 0.211 L-Asparagine • H2O 0.01501 0.01501 L-Aspartic Acid 0.0133 0.0133 L-Cysteine • HCl • H2O 0.035 0.035 L-Glutamic Acid 0.0147 0.0147 L-Glutamine 0.146 0.146 Glycine 0.00751 0.00751 L-Histidine • HCl • H2O 0.021 0.021 L-Isoleucine 0.0026 0.0026 L-Leucine 0.0131 0.0131 L-Lysine • HCl 0.0293 0.0293 L-Methionine 0.00448 0.00488 L-Phenylalanine 0.00496 0.00496 L-Proline 0.0115 0.0115 L-Serine 0.0105 0.0105 L-Threonine 0.00357 0.00357 L-Tryptophan 0.0006 0.0006 L-Tyrosine • 2Na • 2H2O 0.00261 0.00261 L-Valine 0.0035 0.0035 Vitamins D-Biotin 0.000024 0.000024 Choline Chloride 0.000698 0.000698 Folic Acid 0.00132 0.00132 myo-Inositol 0.000541 0.000541 Niacinamide 0.000615 0.000615 D-Pantothenic Acid (hemicalcium) 0.000715 0.000715 Pyridoxine • HCl 0.000206 0.000206 Riboflavin 0.000376 0.000376 Thiamine • HCl 0.001 0.001 Vitamin B12 0.00136 0.00136 Other D-Glucose 1.1 1.1 Hypoxanthine 0.00408 0.00408 Phenol Red • Na 0.0013 0.0013 Pyruvic Acid • Na 0.11 0.11 Thioctic Acid 0.00021 0.00021 Thymidine 0.00073 0.00073 Add Sodium Bicarbonate 1.2 — back to top