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Cell Culture

Nutrient Mixture F-10 Ham Formulation

Component N2147
[1x] g/L
N6013
g/L
Inorganic Salts
Calcium Chloride 0.0333 0.0333
Cupric Sulfate • 5H2O 0.0000025 0.0000025
Ferrous Sulfate • 7H2O 0.000834 0.000834
Magnesium Sulfate (anhydrous) 0.07464 0.07464
Potassium Chloride 0.285 0.285
Potassium Phosphate Monobasic (anhydrous) 0.083 0.083
Sodium Bicarbonate — 1.2
Sodium Chloride 7.4 7.4
Sodium Phosphate Dibasic (anhydrous) 0.1537 0.1537
Zinc Sulfate • 7H2O 0.0000288 0.0000288
Amino Acids
L-Alanine 0.009 0.009
L-Arginine • HCl 0.211 0.211
L-Asparagine • H2O 0.01501 0.01501
L-Aspartic Acid 0.0133 0.0133
L-Cysteine • HCl • H2O 0.035 0.035
L-Glutamic Acid 0.0147 0.0147
Glycine 0.00751 0.00751
L-Histidine • 3HCl • H2O 0.021 0.021
L-Isoleucine 0.0026 0.0026
L-Leucine 0.0131 0.0131
L-Lysine • HCl 0.0293 0.0293
L-Methionine 0.00448 0.00448
L-Phenylalanine 0.00496 0.00496
L-Proline 0.0115 0.0115
L-Serine 0.0105 0.0105
L-Threonine 0.00357 0.00357
L-Tryptophan 0.0006 0.0006
L-Tyrosine • 2Na • 2H2O 0.00261 0.00261
L-Valine 0.0035 0.0035
Vitamins
D-Biotin 0.000024 0.000024
Choline Chloride 0.000698 0.000698
Folic Acid 0.00132 0.00132
myo-Inositol 0.000541 0.000541
Niacinamide 0.000615 0.000615
D-Pantothenic Acid (hemicalcium) 0.000715 0.000715
Pyridoxine • HCl 0.000206 0.000206
Riboflavin 0.000376 0.000376
Thiamine • HCl 0.001 0.001
Vitamin B12 0.00136 0.00136
Other
D-Glucose 1.1 1.1
HEPES 4.77 —
Hypoxanthine 0.00408 0.00408
Phenol Red (sodium) 0.0013 0.0013
Pyruvic Acid (sodium) 0.11 0.11
Thioctic Acid 0.00021 0.00021
Thymidine 0.00073 0.00073
Add
L-Glutamine 0.146 0.146

Component N6635
g/L
N6908
[1x] g/L
Inorganic Salts
Calcium Chloride 0.0333 0.0333
Cupric Sulfate • 5H2O 0.0000025 0.000025
Ferrous Sulfate • 7H2O 0.000834 0.000834
Magnesium Sulfate (anhydrous) 0.07464 0.07464
Potassium Chloride 0.285 0.285
Potassium Phosphate Monobasic (anhydrous) 0.083 0.083
Sodium Bicarbonate — 1.2
Sodium Chloride 7.4 7.4
Sodium Phosphate Dibasic (anhydrous) 0.1537 0.1537
Zinc Sulfate • 7H2O 0.0000288 0.0000288
Amino Acids
L-Alanine 0.009 0.009
L-Arginine • HCl 0.211 0.211
L-Asparagine • H2O 0.01501 0.01501
L-Aspartic Acid 0.0133 0.0133
L-Cysteine • HCl • H2O 0.035 0.035
L-Glutamic Acid 0.0147 0.0147
L-Glutamine 0.146 0.146
Glycine 0.00751 0.00751
L-Histidine • HCl • H2O 0.021 0.021
L-Isoleucine 0.0026 0.0026
L-Leucine 0.0131 0.0131
L-Lysine • HCl 0.0293 0.0293
L-Methionine 0.00448 0.00488
L-Phenylalanine 0.00496 0.00496
L-Proline 0.0115 0.0115
L-Serine 0.0105 0.0105
L-Threonine 0.00357 0.00357
L-Tryptophan 0.0006 0.0006
L-Tyrosine • 2Na • 2H2O 0.00261 0.00261
L-Valine 0.0035 0.0035
Vitamins
D-Biotin 0.000024 0.000024
Choline Chloride 0.000698 0.000698
Folic Acid 0.00132 0.00132
myo-Inositol 0.000541 0.000541
Niacinamide 0.000615 0.000615
D-Pantothenic Acid (hemicalcium) 0.000715 0.000715
Pyridoxine • HCl 0.000206 0.000206
Riboflavin 0.000376 0.000376
Thiamine • HCl 0.001 0.001
Vitamin B12 0.00136 0.00136
Other
D-Glucose 1.1 1.1
Hypoxanthine 0.00408 0.00408
Phenol Red • Na 0.0013 0.0013
Pyruvic Acid • Na 0.11 0.11
Thioctic Acid 0.00021 0.00021
Thymidine 0.00073 0.00073
Add
Sodium Bicarbonate 1.2 —
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