Cell Culture

F-12 Coon's Modification Formulation

Component F6636
g/L
Inorganic Salts
Calcium Chloride 0.1245
Cupric Sulfate • 5H2O 0.0000025
Ferrrous Sulfate • 7H2O 0.000834
Magnesium Chloride • 6H2O 0.046659
Magnesium Sulfate (anhydrous) 0.02528
Potassium Chloride 0.305
Potassium Phosphate Monobasic 0.06124
Sodium Chloride 7.517
Sodium Phosphate Dibasic (anhydrous) 0.1324
Zinc Sulfate • 7H2O 0.000863
Amino Acids
L-Alanine 0.018
L-Arginine • HCl 0.422
L-Asparagine • H2O 0.03
L-Aspartic Acid 0.026
L-Cysteine • HCl • H2O 0.07026
L-Glutamic Acid 0.03
L-Glutamine 0.292
Glycine 0.016
L-Histidine • HCl • H2O 0.042
L-Isoleucine 0.0078
L-Leucine 0.0262
L-Lysine • HCl 0.073
L-Methionine 0.009
L-Phenylalanine 0.01
L-Proline 0.07
L-Serine 0.021
L-Threonine 0.0238
L-Tryptophan 0.004
L-Tyrosine • 2Na • 2H2O 0.01586
L-Valine 0.0234
Vitamins
L-Ascorbic Acid 0.15
D-Biotin 0.0000073
Choline Chloride 0.01396
Folic Acid 0.00132
myo-Inositol 0.01802
Niacinamide 0.00004
L-Pantothenic Acid (hemicalcium) 0.000238
Pyridoxine • HCl 0.00006
Riboflavin 0.00004
Thiamine • HCl 0.000337
Vitamin B12 0.00136
Other
D-Glucose 1.802
Hypoxanthine 0.00404
Linoleic Acid 0.00009
Phenol Red • Na 0.00125
Putrescine • 2HCl 0.000161
Pyruvic Acid • Na 0.22
Thioctic Acid 0.000206
Thymidine 0.0007
Add
Sodium Bicarbonate 2.676
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