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Life Science > Cell Culture > Learning Center > Media Formulations > Nutrient Mixture F-12 Ham Formulation
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Nutrient Mixture F-12 Ham Formulation

Nutrient Mixture F-12 Ham

Component N4888
[1x] g/L		 N6658
[1x] g/L		 N6760
g/L
Inorganic Salts
Calcium Chloride 0.0333 0.0333 0.0333
Cupric Sulfate • 5H2O 0.0000025 0.0000025 0.0000025
Ferrous Sulfate • 7H2O 0.000834 0.000834 0.000834
Magnesium Chloride 0.0576 0.0576 0.0576
Potassium Chloride 0.224 0.224 0.224
Sodium Bicarbonate 1.176 1.176 —
Sodium Chloride 7.599 7.599 7.599
Sodium Phosphate Dibasic (anhydrous) 0.14204 0.14204 0.14204
Zinc Sulfate • 7H2O 0.000863 0.000863 0.000863
Amino Acids
L-Alanine 0.009 0.009 0.009
L-Arginine • HCl 0.211 0.211 0.211
L-Asparagine • H2O 0.01501 0.01501 0.01501
L-Aspartic Acid 0.0133 0.0133 0.0133
L-Cysteine • HCl • H2O 0.035 0.035 0.035
L-Glutamic Acid 0.0147 0.0147 0.0147
L-Glutamine — 0.146 0.146
Glycine 0.00751 0.00751 0.00751
L-Histidine • 3HCl • H2O 0.02096 0.02096 0.02096
L-Isoleucine 0.00394 0.00394 0.00394
L-Leucine 0.0131 0.0131 0.0131
L-Lysine • HCl 0.0365 0.0365 0.0365
L-Methionine 0.00448 0.00448 0.00448
L-Phenylalanine 0.00496 0.00496 0.00496
L-Proline 0.0345 0.0345 0.345
L-Serine 0.0105 0.0105 0.0105
L-Threonine 0.0119 0.0119 0.0119
L-Tryptophan 0.00204 0.00204 0.00204
L-Tyrosine • 2Na • 2H2O 0.00778 0.00778 0.00778
L-Valine 0.0117 0.0117 0.0117
Vitamins
D-Biotin 0.0000073 0.0000073 0.000073
Choline Chloride 0.01396 0.01396 0.01396
Folic Acid 0.00132 0.00132 0.00132
myo-Inositol 0.018 0.018 0.018
Niacinamide 0.000037 0.000037 0.000037
D-Pantothenic Acid (hemicalcium) 0.00048 0.00048 0.00048
Pyridoxine • HCl 0.000062 0.000062 0.000062
Riboflavin 0.000038 0.000038 0.000038
Thiamine • HCl 0.00034 0.00034 0.00034
Vitamin B12 0.00136 0.00136 0.00136
Other
D-Glucose 1.802 1.802 1.802
Hypoxanthine 0.00408 0.00408 0.00408
Linoleic Acid 0.000084 0.000084 0.000084
Phenol Red • Na 0.0013 0.0013 0.0013
Putrescine • HCl 0.000161 0.000161 0.000161
Pyruvic Acid • Na 0.11 0.11 0.11
Thioctic Acid 0.00021 0.00021 0.00021
Thymidine 0.00073 0.00073 0.00073
Add
L-Glutamine 0.146 — —
Sodium Bicarbonate — — 1.176
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Nutrient Mixture F-12 Ham HEPES Modification

Component N8641
[1x] g/L
Inorganic Salts
Calcium Chloride 0.0333
Cupric Sulfate • 5H2O 0.0000025
Ferrous Sulfate • 7H2O 0.000834
Magnesium Chloride 0.0576
Potassium Chloride 0.224
Sodium Bicarbonate 1.176
Sodium Chloride 7.1
Sodium Phosphate Dibasic (anhydrous) 0.14204
Zinc Sulfate • 7H2O 0.000863
Amino Acids
L-Alanine 0.009
L-Arginine • HCl 0.211
L-Asparagine • H2O 0.01501
L-Aspartic Acid 0.0133
L-Cysteine • HCl • H2O 0.035
L-Glutamic Acid 0.0147
Glycine 0.00751
L-Histidine • 3HCl • H2O 0.02096
L-Isoleucine 0.00394
L-Leucine 0.0131
L-Lysine • HCl 0.0365
L-Methionine 0.00448
L-Phenylalanine 0.00496
L-Proline 0.0345
L-Serine 0.0105
L-Threonine 0.0119
L-Tryptophan 0.00204
L-Tyrosine • 2Na • 2H2O 0.00778
L-Valine 0.0117
Vitamins
D-Biotin 0.0000073
Choline Chloride 0.01396
Folic Acid 0.00132
myo-Inositol 0.018
Niacinamide 0.000037
D-Pantothenic Acid (hemicalcium) 0.000238
Pyridoxine • HCl 0.000062
Riboflavin 0.000038
Thiamine • HCl 0.00034
Vitamin B12 0.00136
Other
D-Glucose 1.802
HEPES 5.958
Hypoxanthine 0.00408
Linoleic Acid 0.000084
Phenol Red • Na 0.0013
Putrescine • HCl 1.000161
Pyruvic Acid • Na 0.11
Thioctic Acid 0.00021
Thymidine 0.00073
Add
L-Glutamine 0.146
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Nutrient Mixture F-12 Ham Kaighn's Modification

Component N3520
[1x] g/L
Inorganic Salts
Calcium Chloride 0.10207
Cupric Sulfate • 5H2O 0.0000025
Ferrous Sulfate • 7H2O 0.000834
Magnesium Chloride 0.04961
Magnesium Sulfate 0.19264
Potassium Chloride 0.28329
Potassium Phosphate Monobasic 0.058523
Sodium Phosphate Dibasic 0.11502
Sodium Chloride 7.5972
Zinc Sulfate • 7H2O 0.00014375
Amino Acids
L-Alanine 0.017818
L-Arginine 0.4214
L-Asparagine • H2O 0.03002
L-Aspartic Acid 0.02662
L-Cysteine • HCl • H2O 0.07024
L-Glutamic Acid 0.02942
L-Glutamine 0.2922
Glycine 0.015014
L-Histidine • HCl • H2O 0.04192
L-Isoleucine 0.007872
L-Leucine 0.02624
L-Lysine • HCl 0.07304
L-Methionine 0.008952
L-Phenylalanine 0.009912
L-Proline 0.06906
L-Serine 0.02102
L-Threonine 0.02382
L-Tryptophan 0.004084
L-Tyrosine 0.010872
L-Valine 0.02342
Vitamins
D-Biotin 0.00007329
Choline Chloride 0.01396
Folic Acid 0.0013242
Hypoxanthine 0.004083
myo-Inositol 0.01802
Niacinamide 0.00003663
D-Pantothenic Acid (hemicalcium) 0.000477
Putrescine • 2HCl 0.0003222
Pyridoxine • HCl 0.00006168
Riboflavin 0.00003764
Thiamine • HCl 0.0003373
Thymidine 0.0007266
Vitamin B12 0.0013554
Other
D+-Glucose 1.26
Phenol Red • Na 0.00331806
Pyruvic Acid • Na 0.22
DL-6,8-Thioctic Acid 0.0002063
Add
Sodium Bicarbonate 2.5
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