Cell Culture

Nutrient Mixture F-12 Ham Formulation

Nutrient Mixture F-12 Ham

Component N4888
[1x] g/L
N6658
[1x] g/L
N6760
g/L
Inorganic Salts
Calcium Chloride 0.0333 0.0333 0.0333
Cupric Sulfate • 5H2O 0.0000025 0.0000025 0.0000025
Ferrous Sulfate • 7H2O 0.000834 0.000834 0.000834
Magnesium Chloride 0.0576 0.0576 0.0576
Potassium Chloride 0.224 0.224 0.224
Sodium Bicarbonate 1.176 1.176
Sodium Chloride 7.599 7.599 7.599
Sodium Phosphate Dibasic (anhydrous) 0.14204 0.14204 0.14204
Zinc Sulfate • 7H2O 0.000863 0.000863 0.000863
Amino Acids
L-Alanine 0.009 0.009 0.009
L-Arginine • HCl 0.211 0.211 0.211
L-Asparagine • H2O 0.01501 0.01501 0.01501
L-Aspartic Acid 0.0133 0.0133 0.0133
L-Cysteine • HCl • H2O 0.035 0.035 0.035
L-Glutamic Acid 0.0147 0.0147 0.0147
L-Glutamine 0.146 0.146
Glycine 0.00751 0.00751 0.00751
L-Histidine • 3HCl • H2O 0.02096 0.02096 0.02096
L-Isoleucine 0.00394 0.00394 0.00394
L-Leucine 0.0131 0.0131 0.0131
L-Lysine • HCl 0.0365 0.0365 0.0365
L-Methionine 0.00448 0.00448 0.00448
L-Phenylalanine 0.00496 0.00496 0.00496
L-Proline 0.0345 0.0345 0.345
L-Serine 0.0105 0.0105 0.0105
L-Threonine 0.0119 0.0119 0.0119
L-Tryptophan 0.00204 0.00204 0.00204
L-Tyrosine • 2Na • 2H2O 0.00778 0.00778 0.00778
L-Valine 0.0117 0.0117 0.0117
Vitamins
D-Biotin 0.0000073 0.0000073 0.000073
Choline Chloride 0.01396 0.01396 0.01396
Folic Acid 0.00132 0.00132 0.00132
myo-Inositol 0.018 0.018 0.018
Niacinamide 0.000037 0.000037 0.000037
D-Pantothenic Acid (hemicalcium) 0.00048 0.00048 0.00048
Pyridoxine • HCl 0.000062 0.000062 0.000062
Riboflavin 0.000038 0.000038 0.000038
Thiamine • HCl 0.00034 0.00034 0.00034
Vitamin B12 0.00136 0.00136 0.00136
Other
D-Glucose 1.802 1.802 1.802
Hypoxanthine 0.00408 0.00408 0.00408
Linoleic Acid 0.000084 0.000084 0.000084
Phenol Red • Na 0.0013 0.0013 0.0013
Putrescine • HCl 0.000161 0.000161 0.000161
Pyruvic Acid • Na 0.11 0.11 0.11
Thioctic Acid 0.00021 0.00021 0.00021
Thymidine 0.00073 0.00073 0.00073
Add
L-Glutamine 0.146
Sodium Bicarbonate 1.176
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Nutrient Mixture F-12 Ham HEPES Modification

Component N8641
[1x] g/L
Inorganic Salts
Calcium Chloride 0.0333
Cupric Sulfate • 5H2O 0.0000025
Ferrous Sulfate • 7H2O 0.000834
Magnesium Chloride 0.0576
Potassium Chloride 0.224
Sodium Bicarbonate 1.176
Sodium Chloride 7.1
Sodium Phosphate Dibasic (anhydrous) 0.14204
Zinc Sulfate • 7H2O 0.000863
Amino Acids
L-Alanine 0.009
L-Arginine • HCl 0.211
L-Asparagine • H2O 0.01501
L-Aspartic Acid 0.0133
L-Cysteine • HCl • H2O 0.035
L-Glutamic Acid 0.0147
Glycine 0.00751
L-Histidine • 3HCl • H2O 0.02096
L-Isoleucine 0.00394
L-Leucine 0.0131
L-Lysine • HCl 0.0365
L-Methionine 0.00448
L-Phenylalanine 0.00496
L-Proline 0.0345
L-Serine 0.0105
L-Threonine 0.0119
L-Tryptophan 0.00204
L-Tyrosine • 2Na • 2H2O 0.00778
L-Valine 0.0117
Vitamins
D-Biotin 0.0000073
Choline Chloride 0.01396
Folic Acid 0.00132
myo-Inositol 0.018
Niacinamide 0.000037
D-Pantothenic Acid (hemicalcium) 0.000238
Pyridoxine • HCl 0.000062
Riboflavin 0.000038
Thiamine • HCl 0.00034
Vitamin B12 0.00136
Other
D-Glucose 1.802
HEPES 5.958
Hypoxanthine 0.00408
Linoleic Acid 0.000084
Phenol Red • Na 0.0013
Putrescine • HCl 1.000161
Pyruvic Acid • Na 0.11
Thioctic Acid 0.00021
Thymidine 0.00073
Add
L-Glutamine 0.146
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Nutrient Mixture F-12 Ham Kaighn's Modification

Component N3520
[1x] g/L
Inorganic Salts
Calcium Chloride 0.10207
Cupric Sulfate • 5H2O 0.0000025
Ferrous Sulfate • 7H2O 0.000834
Magnesium Chloride 0.04961
Magnesium Sulfate 0.19264
Potassium Chloride 0.28329
Potassium Phosphate Monobasic 0.058523
Sodium Phosphate Dibasic 0.11502
Sodium Chloride 7.5972
Zinc Sulfate • 7H2O 0.00014375
Amino Acids
L-Alanine 0.017818
L-Arginine 0.4214
L-Asparagine • H2O 0.03002
L-Aspartic Acid 0.02662
L-Cysteine • HCl • H2O 0.07024
L-Glutamic Acid 0.02942
L-Glutamine 0.2922
Glycine 0.015014
L-Histidine • HCl • H2O 0.04192
L-Isoleucine 0.007872
L-Leucine 0.02624
L-Lysine • HCl 0.07304
L-Methionine 0.008952
L-Phenylalanine 0.009912
L-Proline 0.06906
L-Serine 0.02102
L-Threonine 0.02382
L-Tryptophan 0.004084
L-Tyrosine 0.010872
L-Valine 0.02342
Vitamins
D-Biotin 0.00007329
Choline Chloride 0.01396
Folic Acid 0.0013242
Hypoxanthine 0.004083
myo-Inositol 0.01802
Niacinamide 0.00003663
D-Pantothenic Acid (hemicalcium) 0.000477
Putrescine • 2HCl 0.0003222
Pyridoxine • HCl 0.00006168
Riboflavin 0.00003764
Thiamine • HCl 0.0003373
Thymidine 0.0007266
Vitamin B12 0.0013554
Other
D+-Glucose 1.26
Phenol Red • Na 0.00331806
Pyruvic Acid • Na 0.22
DL-6,8-Thioctic Acid 0.0002063
Add
Sodium Bicarbonate 2.5
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