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Cell Culture
Glasgow Minimum Essential Medium Formulation
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|
| Component |
G5154
[1x] g/L |
G6148
g/L |
| Inorganic Salts |
| Calcium Chloride |
0.2 |
0.2 |
| Ferric Nitrate • 9H2O |
0.0001 |
0.0001 |
| Magnesium Sulfate (anhydrous) |
0.09767 |
0.09767 |
| Potassium Chloride |
0.4 |
0.4 |
| Sodium Bicarbonate |
2.75 |
— |
| Sodium Chloride |
6.4 |
6.4 |
| Sodium Phosphate Monobasic (anhydrous) |
0.1078 |
0.1078 |
| Amino Acids |
| L-Arginine • HCl |
0.042 |
0.042 |
| L-Cystine • 2HCl |
0.03129 |
0.03129 |
| L-Glutamine |
— |
0.292 |
| L-Histidine • HCl • H2O |
0.021 |
0.021 |
| L-Isoleucine |
0.0524 |
0.0524 |
| L-Leucine |
0.0524 |
0.0524 |
| L-Lysine • HCl |
0.0731 |
0.0731 |
| L-Methionine |
0.015 |
0.015 |
| L-Phenylalanine |
0.033 |
0.033 |
| L-Threonine |
0.0476 |
0.0476 |
| L-Tryptophan |
0.008 |
0.008 |
| L-Tyrosine • 2Na • H2O |
0.05219 |
0.05219 |
| L-Valine |
0.0468 |
0.0468 |
| Vitamins |
| Choline Chloride |
0.002 |
0.002 |
| Folic Acid |
0.002 |
0.002 |
| myo-Inositol |
0.0036 |
0.0036 |
| Niacinamide |
0.002 |
0.002 |
| D-Pantothenic Acid (hemicalcium) |
0.002 |
0.002 |
| Pyridoxal • HCl |
0.002 |
0.002 |
| Riboflavin |
0.0002 |
0.0002 |
| Thiamine • HCl |
0.002 |
0.002 |
| Other |
| D-Glucose |
4.5 |
4.5 |
| Phenol Red • Na |
0.016 |
0.016 |
| Add |
| Sodium Bicarbonate |
— |
2.75 |
| L-Glutamine |
0.292 |
— |
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