Cell Culture

Iscove's Modified Dulbecco's Media (IMDM) Formulation

Component I3390
[1x] g/L
I7633
g/L
Inorganic Salts
Calcium Chloride 0.1653 0.1653
Magnesium Sulfate (anhydrous) 0.09767 0.9767
Potassium Chloride 0.33 0.33
Potassium Nitrate 0.000076 0.000076
Sodium Bicarbonate 3.024
Sodium Chloride 4.505 4.505
Sodium Phosphate Monobasic (anhydrous) 0.109 0.109
Sodium Selenite 0.000017 0.000017
Amino Acids
L-Alanine 0.025 0.025
L-Arginine • HCl 0.084 0.084
L-Asparagine • H2O 0.0284 0.0284
L-Aspartic Acid 0.03 0.03
L-Cystine • 2HCl 0.09124 0.09124
L-Glutamic Acid 0.075 0.075
L-Glutamine 0.584
Glycine 0.03 0.03
L-Histidine • HCl • H2O 0.042 0.042
L-Isoleucine 0.105 0.105
L-Leucine 0.105 0.105
L-Lysine • HCl 0.146 0.146
L-Methionine 0.03 0.03
L-Phenylalanine 0.066 0.066
L-Proline 0.04 0.04
L-Serine 0.042 0.042
L-Threonine 0.095 0.095
L-Tryptophan 0.016 0.016
L-Tyrosine • 2Na • 2H2O 0.10379 0.10379
L-Valine 0.094 0.094
Vitamins
D-Biotin 0.000013 0.000013
Choline Chloride 0.004 0.004
Folic Acid 0.004 0.004
myo-Inositol 0.0072 0.0072
Niacinamide 0.004 0.004
D-Pantothenic Acid (hemicalcium) 0.004 0.004
Pyridoxal • HCl 0.004 0.004
Riboflavin 0.0004 0.0004
Thiamine • HCl 0.004 0.004
Vitamin B12 0.000013 0.000013
Other
D-Glucose 4.5 4.5
HEPES 5.958 5.958
Phenol Red • Na 0.016 0.016
Pyruvic Acid • Na 0.11 0.11
Add
Sodium Bicarbonate 3.024
L-Glutamine 0.584
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