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Cell Culture
Iscove's Modified Dulbecco's Media (IMDM) Formulation
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| Component |
I3390
[1x] g/L |
I7633
g/L |
| Inorganic Salts |
| Calcium Chloride |
0.1653 |
0.1653 |
| Magnesium Sulfate (anhydrous) |
0.09767 |
0.9767 |
| Potassium Chloride |
0.33 |
0.33 |
| Potassium Nitrate |
0.000076 |
0.000076 |
| Sodium Bicarbonate |
3.024 |
— |
| Sodium Chloride |
4.505 |
4.505 |
| Sodium Phosphate Monobasic (anhydrous) |
0.109 |
0.109 |
| Sodium Selenite |
0.000017 |
0.000017 |
| Amino Acids |
| L-Alanine |
0.025 |
0.025 |
| L-Arginine • HCl |
0.084 |
0.084 |
| L-Asparagine • H2O |
0.0284 |
0.0284 |
| L-Aspartic Acid |
0.03 |
0.03 |
| L-Cystine • 2HCl |
0.09124 |
0.09124 |
| L-Glutamic Acid |
0.075 |
0.075 |
| L-Glutamine |
— |
0.584 |
| Glycine |
0.03 |
0.03 |
| L-Histidine • HCl • H2O |
0.042 |
0.042 |
| L-Isoleucine |
0.105 |
0.105 |
| L-Leucine |
0.105 |
0.105 |
| L-Lysine • HCl |
0.146 |
0.146 |
| L-Methionine |
0.03 |
0.03 |
| L-Phenylalanine |
0.066 |
0.066 |
| L-Proline |
0.04 |
0.04 |
| L-Serine |
0.042 |
0.042 |
| L-Threonine |
0.095 |
0.095 |
| L-Tryptophan |
0.016 |
0.016 |
| L-Tyrosine • 2Na • 2H2O |
0.10379 |
0.10379 |
| L-Valine |
0.094 |
0.094 |
| Vitamins |
| D-Biotin |
0.000013 |
0.000013 |
| Choline Chloride |
0.004 |
0.004 |
| Folic Acid |
0.004 |
0.004 |
| myo-Inositol |
0.0072 |
0.0072 |
| Niacinamide |
0.004 |
0.004 |
| D-Pantothenic Acid (hemicalcium) |
0.004 |
0.004 |
| Pyridoxal • HCl |
0.004 |
0.004 |
| Riboflavin |
0.0004 |
0.0004 |
| Thiamine • HCl |
0.004 |
0.004 |
| Vitamin B12 |
0.000013 |
0.000013 |
| Other |
| D-Glucose |
4.5 |
4.5 |
| HEPES |
5.958 |
5.958 |
| Phenol Red • Na |
0.016 |
0.016 |
| Pyruvic Acid • Na |
0.11 |
0.11 |
| Add |
| Sodium Bicarbonate |
— |
3.024 |
| L-Glutamine |
0.584 |
— |
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