Iscove's Modified Dulbecco's Media (IMDM) Formulation

Component	I3390 [1x] g/L	I7633 g/L
Inorganic Salts
Calcium Chloride	0.1653	0.1653
Magnesium Sulfate (anhydrous)	0.09767	0.9767
Potassium Chloride	0.33	0.33
Potassium Nitrate	0.000076	0.000076
Sodium Bicarbonate	3.024	—
Sodium Chloride	4.505	4.505
Sodium Phosphate Monobasic (anhydrous)	0.109	0.109
Sodium Selenite	0.000017	0.000017
Amino Acids
L-Alanine	0.025	0.025
L-Arginine • HCl	0.084	0.084
L-Asparagine • H2O	0.0284	0.0284
L-Aspartic Acid	0.03	0.03
L-Cystine • 2HCl	0.09124	0.09124
L-Glutamic Acid	0.075	0.075
L-Glutamine	—	0.584
Glycine	0.03	0.03
L-Histidine • HCl • H2O	0.042	0.042
L-Isoleucine	0.105	0.105
L-Leucine	0.105	0.105
L-Lysine • HCl	0.146	0.146
L-Methionine	0.03	0.03
L-Phenylalanine	0.066	0.066
L-Proline	0.04	0.04
L-Serine	0.042	0.042
L-Threonine	0.095	0.095
L-Tryptophan	0.016	0.016
L-Tyrosine • 2Na • 2H2O	0.10379	0.10379
L-Valine	0.094	0.094
Vitamins
D-Biotin	0.000013	0.000013
Choline Chloride	0.004	0.004
Folic Acid	0.004	0.004
myo-Inositol	0.0072	0.0072
Niacinamide	0.004	0.004
D-Pantothenic Acid (hemicalcium)	0.004	0.004
Pyridoxal • HCl	0.004	0.004
Riboflavin	0.0004	0.0004
Thiamine • HCl	0.004	0.004
Vitamin B12	0.000013	0.000013
Other
D-Glucose	4.5	4.5
HEPES	5.958	5.958
Phenol Red • Na	0.016	0.016
Pyruvic Acid • Na	0.11	0.11
Add
Sodium Bicarbonate	—	3.024
L-Glutamine	0.584	—
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