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Cell Culture
L-15 Media Formulation
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| Component |
L1518
[1x] g/L |
L4386
[1x] g/L |
L5520
g/L |
| Inorganic Salts |
| Calcium Chloride |
0.1396 |
0.1396 |
0.1396 |
| Magnesium Chloride |
0.0937 |
0.0937 |
0.0937 |
| Magnesium Sulfate (anhydrous) |
0.09767 |
0.09767 |
0.09767 |
| Potassium Chloride |
0.4 |
0.4 |
0.4 |
| Potassium Phosphate Monobasic (anhydrous) |
0.06 |
0.06 |
0.06 |
| Sodium Chloride |
8 |
8 |
8 |
| Sodium Phosphate Dibasic (anhydrous) |
0.19 |
0.19 |
0.19 |
| Amino Acids |
| L-Alanine |
0.225 |
0.225 |
0.225 |
| L-Arginine (free base) |
0.5 |
0.5 |
0.5 |
| L-Asparagine (anhydrous) |
0.25 |
0.25 |
0.25 |
| L-Cysteine (free base) |
0.12 |
0.12 |
0.12 |
| L-Glutamine |
0.3 |
0.3 |
— |
| Glycine |
0.2 |
0.2 |
0.2 |
| L-Histidine |
0.25 |
0.25 |
0.25 |
| L-Isoleucine |
0.125 |
0.125 |
0.125 |
| L-Leucine |
0.125 |
0.125 |
0.125 |
| L-Lysine |
0.0937 |
0.0937 |
0.0937 |
| L-Methionine |
0.075 |
0.075 |
0.075 |
| L-Phenylalanine |
0.125 |
0.125 |
0.125 |
| L-Serine |
0.2 |
0.2 |
0.2 |
| L-Threonine |
0.3 |
0.3 |
0.3 |
| L-Tryptophan |
0.02 |
0.02 |
0.02 |
| L-Tyrosine (free base) |
0.3 |
0.3 |
0.3 |
| L-Valine |
0.1 |
0.1 |
0.1 |
| Vitamins |
| Choline Chloride |
0.001 |
0.001 |
0.001 |
| Flavin Mononucleotide • Na |
0.0001 |
0.0001 |
0.0001 |
| Folic Acid |
0.001 |
0.001 |
0.001 |
| myo-Inositol |
0.002 |
0.002 |
0.002 |
| Niacinamide |
0.001 |
0.001 |
0.001 |
| DL-Pantothenic Acid (hemicalcium) |
0.001 |
0.001 |
0.001 |
| Pyridoxine • HCl |
0.001 |
0.001 |
0.001 |
| Thiamine Monophosphate • HCl |
0.001 |
0.001 |
0.001 |
| Other |
| D-Galactose |
0.9 |
0.9 |
0.9 |
| Phenol Red • Na |
0.011 |
0.011 |
0.011 |
| Pyruvic Acid • Na |
0.55 |
0.55 |
0.55 |
| Add |
| L-Glutamine |
— |
— |
0.3 |
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