|
|
Cell Culture
McCoy's 5A Modified Media Formulation
|
|
|
| Component |
M4892
g/L |
M8403
[1x] g/L |
| Inorganic Salts |
| Calcium Chloride |
0.09995 |
0.09995 |
| Magnesium Sulfate (anhydrous) |
0.0976876 |
0.0976876 |
| Potassium Chloride |
0.4 |
0.4 |
| Sodium Bicarbonate |
— |
2.2 |
| Sodium Chloride |
6.46 |
6.46 |
| Sodium Phosphate Monobasic (anhydrous) |
0.504 |
0.504 |
| Amino Acids |
| L-Alanine |
0.01336 |
0.01336 |
| L-Arginine • HCl |
0.04214 |
0.04214 |
| L-Asparagine • H2O |
0.04503 |
0.04503 |
| L-Aspartic Acid |
0.01997 |
0.01997 |
| L-Cysteine |
0.02424 |
0.02424 |
| L-Glutamic Acid |
0.02207 |
0.02207 |
| L-Glutamine |
0.21915 |
— |
| Glycine |
0.00751 |
0.00751 |
| L-Histidine • HCl • H2O |
0.02096 |
0.02096 |
| Hydroxy-l-proline |
0.01967 |
0.01967 |
| L-Isoleucine |
0.03936 |
0.03936 |
| L-Leucine |
0.03936 |
0.03936 |
| L-Lysine • HCl |
0.03654 |
0.03654 |
| L-Methionine |
0.01492 |
0.01492 |
| L-Phenylalanine |
0.01652 |
0.01652 |
| L-Proline |
0.01727 |
0.01727 |
| L-Serine |
0.02628 |
0.02628 |
| L-Threonine |
0.01787 |
0.01787 |
| L-Tryptophan |
0.00306 |
0.00306 |
| L-Tyrosine • 2Na • 2H2O |
0.0261 |
0.0261 |
| L-Valine |
0.01757 |
0.01757 |
| Vitamins |
| Ascorbic Acid |
0.0005625 |
0.0005625 |
| p-Aminobenzoic Acid |
0.001 |
0.001 |
| D-Biotin |
0.0002 |
0.0002 |
| Choline Chloride |
0.005 |
0.005 |
| Folic Acid |
0.01 |
0.01 |
| myo-Inositol |
0.036 |
0.036 |
| Niacinamide |
0.0005 |
0.0005 |
| Nicotinic Acid |
0.0005 |
0.0005 |
| D-Pantothenic Acid (hemicalcium) |
0.0002 |
0.0002 |
| Pyridoxal • HCl |
0.0005 |
0.0005 |
| Pyridoxine • HCl |
0.0005 |
0.0005 |
| Riboflavin |
0.0002 |
0.0002 |
| Thiamine • HCl |
0.0002 |
0.0002 |
| Vitamin B12 |
0.002 |
0.002 |
| Other |
| Peptone |
0.6 |
0.6 |
| D-Glucose |
3 |
3 |
| Glutathione (reduced) |
0.0005 |
0.0005 |
| Phenol Red • Na |
0.011 |
0.011 |
| Add |
| Sodium Bicarbonate |
2.2 |
— |
| L-Glutamine |
— |
0.21915 |
|
