Minimum Essential Medium Eagle (MEM) Formulation

• Minimum Essential Medium Eagle (MEM)
• Minimum Essential Medium Eagle (MEM) Alpha Modification
• Minimum Essential Medium Eagle (MEM) HEPES Modification
• Minimum Essential Medium Eagle (MEM) Joklik Modification for Suspension Cultures
• Minimum Essential Medium Eagle (MEM) Modified
• Minimum Essential Medium Eagle (MEM) Auto-Mod™ Modified for Autoclaving
• Minimum Essential Medium Eagle (S-MEM) Spinner Modification

Minimum Essential Medium Eagle (MEM)

Component	M0268 g/L	M0275 [10x] g/L	M0446 [1x] g/L	M0643 g/L	M1018 g/L
Inorganic Salts
Calcium Chloride	0.2	2	0.2	0.2	0.1396
Magnesium Sulfate (anhydrous)	0.09767	0.9767	0.09767	0.09767	0.09767
Potassium Chloride	0.4	4	0.4	0.4	0.4
Potassium Phosphate Monobasic (anhydrous)	—	—	—	—	0.06
Sodium Bicarbonate	—	—	2.2	—	—
Sodium Chloride	6.8	68	6.8	6.8	8
Sodium Phosphate Dibasic (anhydrous)	—	—	—	—	0.04788
Sodium Phosphate Monobasic (anhydrous)	0.122	1.22	0.122	0.122	—
Amino Acids
L-Alanine	—	—	—	0.0089	0.0089
L-Alanyl-L-Glutamine	—	—	0.4344	—	—
L-Arginine • HCl	0.126	1.26	0.126	0.126	0.126
L-Asparagine • H2O	—	—	—	0.015	0.015
L-Aspartic Acid	—	—	—	0.0133	0.0133
L-Cystine • 2HCl	0.0313	0.313	0.0313	0.0313	0.0313
L-Glutamic Acid	—	—	—	0.0147	0.0147
L-Glutamine	0.292	—	0.292	0.292	0.292
Glycine	—	—	—	0.0075	0.0075
L-Histidine • HCl • H2O	0.042	0.42	0.042	0.042	0.042
L-Isoleucine	0.052	0.52	0.052	0.052	0.052
L-Leucine	0.052	0.52	0.052	0.052	0.052
L-Lysine • HCl	0.0725	0.725	0.0725	0.0725	0.0725
L-Methionine	0.015	0.15	0.015	0.015	0.015
L-Phenylalanine	0.032	0.32	0.032	0.032	0.032
L-Proline	—	—	—	0.0115	0.0115
L-Serine	—	—	—	0.0105	0.0105
L-Threonine	0.048	0.48	0.048	0.048	0.048
L-Tryptophan	0.01	0.1	0.01	0.01	0.01
L-Tyrosine • 2Na • 2H2O	0.0519	0.519	0.0519	0.0519	0.0519
L-Valine	0.046	0.46	0.046	0.046	0.046
Vitamins
Choline Chloride	0.001	0.01	0.001	0.001	0.001
Folic Acid	0.001	0.01	0.001	0.001	0.001
myo-Inositol	0.002	0.02	0.002	0.002	0.002
Niacinamide	0.001	0.01	0.001	0.001	0.001
D-Pantothenic Acid (hemicalcium)	0.001	0.01	0.001	0.001	0.001
Pyridoxal • HCl	0.001	0.01	0.001	0.001	0.001
Riboflavin	0.0001	0.001	0.0001	0.0001	0.0001
Thiamine • HCl	0.001	0.01	0.001	0.001	0.001
Other
Glucose	1	10	1	1	1
Phenol Red • Na	0.011	0.11	0.011	0.011	0.011
Add
L-Glutamine	—	0.292 at 1×	—	—	—
Sodium Bicarbonate	2.2	2.2 at 1×	—	2.2	0.35
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Minimum Essential Medium Eagle (MEM) (cont.)

Component	M2279 g/L	M2414 [1x] g/L	M4642 [1x] g/L	M4655 g/L
Inorganic Salts
Calcium Chloride	0.2	0.2	0.2	0.2
Magnesium Sulfate (anhydrous)	0.09767	0.09767	0.09767	0.09767
Potassium Chloride	0.4	0.4	0.4	0.4
Potassium Phosphate Monobasic (anhydrous)	—	—	0.06	—
Sodium Bicarbonate	—	2.2	—	2.2
Sodium Chloride	6.8	6.8	8	6.8
Sodium Phosphate Dibasic (anhydrous)	—	—	0.04788	—
Sodium Phosphate Monobasic (anhydrous)	0.122	0.122	—	0.122
Amino Acids
L-Alanine	—	—	—	—
L-Arginine • HCl	0.126	0.126	0.126	0.126
L-Asparagine • H2O	—	—	—	—
L-Aspartic Acid	—	—	—	—
L-Cystine • 2HCl	0.0313	0.0313	0.0313	0.0313
L-Glutamic Acid	—	—	—	—
L-Glutamine	—	0.292	0.292	0.292
Glycine	—	—	—	—
L-Histidine • HCl • H2O	0.042	0.042	0.042	0.042
L-Isoleucine	0.052	0.052	0.052	0.052
L-Leucine	0.052	0.052	0.052	0.052
L-Lysine • HCl	0.0725	0.0725	0.0725	0.0725
L-Methionine	0.015	0.015	0.015	0.015
L-Phenylalanine	0.032	0.032	0.032	0.032
L-Proline	—	—	—	—
L-Serine	—	—	—	—
L-Threonine	0.048	0.048	0.048	0.048
L-Tryptophan	0.01	0.01	0.01	0.01
L-Tyrosine • 2Na • 2H2O	0.0519	0.0519	0.0519	0.0519
L-Valine	0.046	0.046	0.046	0.046
Vitamins
Choline Chloride	0.001	0.001	0.001	0.001
Folic Acid	0.001	0.001	0.001	0.001
myo-Inositol	0.002	0.002	0.002	0.002
Niacinamide	0.001	0.001	0.001	0.001
D-Pantothenic Acid (hemicalcium)	0.001	0.001	0.001	0.001
Pyridoxal • HCl	0.001	0.001	0.001	0.001
Riboflavin	0.0001	0.0001	0.0001	0.0001
Thiamine • HCl	0.001	0.001	0.001	0.001
Other
Glucose	1	1	1	1
Phenol Red • Na	0.011	0.011	0.011	0.011
Add
L-Glutamine	0.292	—	—	—
Sodium Bicarbonate	2.2	—	0.35	—
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Minimum Essential Medium Eagle (MEM) (cont.)

Component	M4780 [1x] g/L	M5650 [1x] g/L	M5775 [1x] g/L	M9288 [10x] g/L
Inorganic Salts
Calcium Chloride	0.1396	0.2	0.1396	1.396
Magnesium Sulfate (anhydrous)	0.09767	0.09767	0.09767	0.9767
Potassium Chloride	0.4	0.4	0.4	4
Potassium Phosphate Monobasic (anhydrous)	0.06	—	0.06	0.6
Sodium Bicarbonate	0.35	2.2	0.35	—
Sodium Chloride	8	6.8	8	80
Sodium Phosphate Dibasic (anhydrous)	0.04788	—	0.04788	0.4788
Sodium Phosphate Monobasic (anhydrous)	—	0.122	—	—
Amino Acids
L-Alanine	—	0.0089	—	—
L-Arginine • HCl	0.126	0.126	0.126	1.26
L-Asparagine • H2O	—	0.015	—	—
L-Aspartic Acid	—	0.0133	—	—
L-Cystine • 2HCl	0.0313	0.0313	0.0313	0.313
L-Glutamic Acid	—	0.0147	—	—
L-Glutamine	0.292	—	—	—
Glycine	—	0.0075	—	—
L-Histidine • HCl • H2O	0.042	0.042	0.042	0.42
L-Isoleucine	0.052	0.052	0.052	0.52
L-Leucine	0.052	0.052	0.052	0.52
L-Lysine • HCl	0.0725	0.0725	0.0725	0.725
L-Methionine	0.015	0.015	0.015	0.15
L-Phenylalanine	0.032	0.032	0.032	0.32
L-Proline	—	0.0115	—	—
L-Serine	—	0.0105	—	—
L-Threonine	0.048	0.048	0.048	0.48
L-Tryptophan	0.01	0.01	0.01	0.1
L-Tyrosine • 2Na • 2H2O	0.0519	0.0519	0.0519	0.519
L-Valine	0.046	0.046	0.046	0.46
Vitamins
Choline Chloride	0.001	0.001	0.001	0.01
Folic Acid	0.001	0.001	0.001	0.01
myo-Inositol	0.002	0.002	0.002	0.02
Niacinamide	0.001	0.001	0.001	0.01
D-Pantothenic Acid (hemicalcium)	0.001	0.001	0.001	0.01
Pyridoxal • HCl	0.001	0.001	0.001	0.01
Riboflavin	0.0001	0.0001	0.0001	0.001
Thiamine • HCl	0.001	0.001	0.001	0.01
Other
Glucose	1	1	1	10
Phenol Red • Na	0.011	0.011	0.011	0.11
Add
L-Glutamine	—	0.292	0.292	0.292 at 1×
Sodium Bicarbonate	—	—	—	0.35 at 1×
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Minimum Essential Medium Eagle (MEM) Alpha Modification

Component	M0644 g/L	M0894 g/L	M4526 [1x] g/L	M8042 [1x] g/L
Inorganic Salts
Calcium Chloride	0.2	0.2	0.2	0.2
Magnesium Sulfate (anhydrous)	0.09767	0.09767	0.09767	0.09767
Potassium Chloride	0.4	0.4	0.4	0.4
Sodium Bicarbonate	—	—	2.2	2.2
Sodium Chloride	6.8	6.8	6.8	6.8
Sodium Phosphate Monobasic (anhydrous)	0.122	0.122	0.122	0.122
Amino Acids
L-Alanine	0.025	0.025	0.025	0.025
L-Arginine • HCl	0.126	0.126	0.126	0.126
L-Asparagine • H2O	0.05	0.05	0.05	0.05
L-Aspartic Acid	0.03	0.03	0.03	0.03
L-Cysteine • HCl • H2O	0.1	0.1	0.1	0.1
L-Cystine • 2HCl	0.0313	0.0313	0.0313	0.0313
L-Glutamic Acid	0.075	0.075	0.075	0.075
L-Glutamine	0.292	0.292	—	—
Glycine	0.05	0.05	0.05	0.05
L-Histidine • HCl • H2O	0.042	0.042	0.042	0.042
L-Isoleucine	0.052	0.052	0.052	0.052
L-Leucine	0.052	0.052	0.052	0.052
L-Lysine • HCl	0.0725	0.0725	0.0725	0.0725
L-Methionine	0.015	0.015	0.015	0.015
L-Phenylalanine	0.032	0.032	0.032	0.032
L-Proline	0.04	0.04	0.04	0.04
L-Serine	0.025	0.025	0.025	0.025
L-Threonine	0.048	0.048	0.048	0.048
L-Tryptophan	0.01	0.01	0.01	0.01
L-Tyrosine • 2Na • 2H2O	0.0519	0.0519	0.0519	0.0519
L-Valine	0.046	0.046	0.046	0.046
Vitamins
L-Ascorbic Acid • Na	0.05	0.05	0.05	0.05
Biotin	0.0001	0.0001	0.0001	0.0001
Choline Chloride	0.001	0.001	0.001	0.001
Folic Acid	0.001	0.001	0.001	0.001
myo-Inositol	0.002	0.002	0.002	0.002
Lipoic Acid	—	—	0.0002	—
Niacinamide	0.001	0.001	0.001	0.001
D-Pantothenic Acid (hemicalcium)	0.001	0.001	0.001	0.001
Pyridoxal • HCl	0.001	0.001	0.001	0.001
Riboflavin	0.0001	0.0001	0.0001	0.0001
Thiamine • HCl	0.001	0.001	0.001	0.001
Vitamin B12	0.00136	0.00136	0.00136	0.00136
Other
Adenosine	0.01	—	—	0.01
Cytidine	0.01	—	—	0.01
2'-Deoxyadenosine	0.01	—	—	0.01
2'-Deoxycytidine • HCl	0.011	—	—	0.011
2'-Deoxyguanosine	0.01	—	—	0.01
Glucose	1	1	1	1
Guanosine	0.01	—	—	0.01
Phenol Red • Na	0.011	0.011	0.011	0.011
Pyruvic Acid • Na	0.11	0.11	0.11	0.11
Thioctic Acid	0.0002	0.0002	—	0.0002
Thymidine	0.01	—	—	0.01
Uridine	0.01	—	—	0.01
Add
L-Glutamine	—	—	0.292	0.292
Sodium Bicarbonate	2.2	2.2	—	—
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Minimum Essential Medium Eagle (MEM) HEPES Modification

Component	M2645 g/L	M7278 [1x] g/L
Inorganic Salts
Calcium Chloride	0.2	0.2
Magnesium Sulfate (anhydrous)	0.09767	0.09767
Potassium Chloride	0.4	0.4
Sodium Bicarbonate	—	2.2
Sodium Chloride	5.5	5.5
Sodium Phosphate Monobasic (anhydrous)	0.122	0.122
Amino Acids
L-Arginine • HCl	0.126	0.126
L-Cystine • 2HCl	0.0313	0.0313
L-Glutamine	0.292	—
L-Histidine • HCl • H2O	0.042	0.042
L-Isoleucine	0.052	0.052
L-Leucine	0.052	0.052
L-Lysine • HCl	0.0725	0.0725
L-Methionine	0.015	0.015
L-Phenylalanine	0.032	0.032
L-Threonine	0.048	0.048
L-Tryptophan	0.01	0.01
L-Tyrosine • 2Na • 2H2O	0.0519	0.0519
L-Valine	0.046	0.046
Vitamins
Choline Chloride	0.001	0.001
Folic Acid	0.001	0.001
myo-Inositol	0.002	0.002
Niacinamide	0.001	0.001
D-Pantothenic Acid (hemicalcium)	0.001	0.001
Pyridoxal • HCl	0.001	0.001
Riboflavin	0.0001	0.0001
Thiamine • HCl	0.001	0.001
Other
Glucose	1	1
HEPES	5.958	5.958
Phenol Red • Na	0.011	0.011
Add
L-Glutamine	—	0.292
Sodium Bicarbonate	2.2	—
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Minimum Essential Medium Eagle (MEM) Joklik Modification for Suspension Cultures

Component	M0518 g/L	M8028 [1x] g/L
Inorganic Salts
Magnesium Chloride • 6H2O	0.2	0.2
Potassium Chloride	0.4	0.4
Sodium Bicarbonate	—	2
Sodium Chloride	6.5	6.5
Sodium Phosphate Monobasic (anhydrous)	1.154	1.154
Amino Acids
L-Arginine • HCl	0.126	1.26
L-Cystine • 2HCl	0.0324	0.0324
L-Glutamine	0.292	—
L-Histidine • HCl • H2O	0.042	0.042
L-Isoleucine	0.052	0.052
L-Leucine	0.052	0.052
L-Lysine • HCl	0.0725	0.0725
L-Methionine	0.015	0.015
L-Phenylalanine	0.032	0.032
L-Threonine	0.048	0.048
L-Tryptophan	0.01	0.01
L-Tyrosine • 2Na • 2H2O	0.05452	0.05452
L-Valine	0.046	0.046
Vitamins
Choline Chloride	0.001	0.001
Folic Acid	0.001	0.001
myo-Inositol	0.002	0.002
Niacinamide	0.001	0.001
D-Pantothenic Acid (hemicalcium)	0.001	0.001
Pyridoxal • HCl	0.001	0.001
Riboflavin	0.0001	0.0001
Thiamine • HCl	0.001	0.001
Other
Glucose	2	2
Phenol Red • Na	0.011	0.011
Add
L-Glutamine	—	0.292
Sodium Bicarbonate	2	—
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Minimum Essential Medium Eagle (MEM) Modified

Component	M3024 g/L
Inorganic Salts
Calcium Chloride	0.2
Magnesium Sulfate (anhydrous)	0.09767
Potassium Chloride	0.4
Sodium Chloride	6.8
Sodium Phosphate Monobasic (anhydrous)	0.122
Amino Acids
L-Alanine	0.0089
L-Arginine • HCl	0.126
L-Asparagine • H2O	0.015
L-Aspartic Acid	0.0133
L-Cystine • 2HCl	0.0313
L-Glutamic Acid	0.0147
Glycine	0.0075
L-Histidine • HCl • H2O	0.042
L-Isoleucine	0.052
L-Leucine	0.052
L-Lysine • HCl	0.0725
L-Methionine	0.015
L-Phenylalanine	0.032
L-Proline	0.0115
L-Serine	0.0105
L-Threonine	0.048
L-Tryptophan	0.01
L-Tyrosine • 2Na • 2H2O	0.0519
L-Valine	0.046
Vitamins
Choline Chloride	0.001
Folic Acid	0.001
myo-Inositol	0.002
Niacinamide	0.001
D-Pantothenic Acid (hemicalcium)	0.001
Pyridoxal • HCl	0.001
Riboflavin	0.0001
Thiamine • HCl	0.001
Other
Glucose	1
Add
L-Glutamine	0.292
Sodium Bicarbonate	2.2
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Minimum Essential Medium Eagle (MEM) Auto-Mod™ Modified for Autoclaving

Component	M0769 g/L
Inorganic Salts
Calcium Chloride	0.2
Magnesium Sulfate (anhydrous)	0.09767
Potassium Chloride	0.4
Sodium Chloride	6.8
Sodium Phosphate Monobasic (anhydrous)	0.122
Sodium Succinate • 6H2O	0.1
Succinic Acid (free acid)	0.075
Amino Acids
L-Arginine • HCl	0.126
L-Cystine • 2HCl	0.0313
L-Histidine • HCl • H2O	0.042
L-Isoleucine	0.052
L-Leucine	0.052
L-Lysine • HCl	0.0725
L-Methionine	0.015
L-Phenylalanine	0.032
L-Threonine	0.048
L-Tryptophan	0.01
L-Tyrosine (free base)	0.036
L-Valine	0.046
Vitamins
Choline Bitartrate	0.0018
Folic Acid	0.001
myo-Inositol	0.002
Niacinamide	0.001
D-Pantothenic Acid (hemicalcium)	0.001
Pyridoxal • HCl	0.001
Riboflavin	0.0001
Thiamine • HCl	0.001
Other
Glucose	1
Phenol Red • Na	0.0064
Add
L-Glutamine	0.292
Sodium Bicarbonate	2.2
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Minimum Essential Medium Eagle (S-MEM) Spinner Modification

Component	M8167 [1x] g/L
Inorganic Salts
Magnesium Sulfate (anhydrous)	0.09767
Potassium Chloride	0.4
Sodium Chloride	6.8
Sodium Phosphate Monobasic (anhydrous)	0.122
Amino Acids
L-Arginine • HCl	0.126
L-Cystine • 2HCl	0.0313
L-Histidine • HCl • H2O	0.042
L-Isoleucine	0.052
L-Leucine	0.052
L-Lysine • HCl	0.0725
L-Methionine	0.015
L-Phenylalanine	0.032
L-Threonine	0.048
L-Tryptophan	0.01
L-Tyrosine • 2Na • 2H2O	0.0519
L-Valine	0.046
Vitamins
Choline Chloride	0.001
Folic Acid	0.001
myo-Inositol	0.002
Niacinamide	0.001
D-Pantothenic Acid (hemicalcium)	0.001
Pyridoxal • HCl	0.001
Riboflavin	0.0001
Thiamine • HCl	0.001
Other
Glucose	1
Phenol Red • Na	0.011
Sodium Bicarbonate	2.2
Add
L-Glutamine	0.292
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