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Cell Culture
NCTC 109 Medium Formulation
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| Component |
N1140
[1x] g/L |
N3262
g/L |
| Inorganic Salts |
| Calcium Chloride |
0.2 |
0.2 |
| Magnesium Sulfate (anhydrous) |
0.1 |
0.1 |
| Potassium Chloride |
0.4 |
0.4 |
| Sodium Acetate (anhydrous) |
0.03 |
0.03 |
| Sodium Bicarbonate |
2.2 |
— |
| Sodium Chloride |
6.8 |
6.8 |
| Sodium Phosphate Monobasic (anhydrous) |
0.122 |
0.122 |
| Amino Acids |
| L-Alanine |
0.03148 |
0.03148 |
| L-Arginine • HCl |
0.03116 |
0.03116 |
| L-Asparagine • H2O |
0.00919 |
0.00919 |
| L-Aspartic Acid |
0.00991 |
0.00991 |
| L-Cysteine • HCl • H2O |
0.28971 |
— |
| L-Cystine • 2HCl |
0.01368 |
0.01368 |
| L-Glutamic Acid |
0.00826 |
0.00826 |
| L-Glutamine |
— |
0.13573 |
| Glycine |
0.01351 |
0.01351 |
| L-Histidine • HCl • H2O |
0.02665 |
0.02665 |
| Hydroxy-L-Proline |
0.00409 |
0.00409 |
| L-Isoleucine |
0.01804 |
0.01804 |
| L-Leucine |
0.02044 |
0.02044 |
| L-Lysine • HCl |
0.03843 |
0.03843 |
| L-Methionine |
0.00444 |
0.00444 |
| L-Ornithine • HCl |
0.00941 |
0.00941 |
| L-Phenylalanine |
0.01653 |
0.01653 |
| L-Proline |
0.00613 |
0.00613 |
| L-Serine |
0.01075 |
0.01075 |
| L-Threonine |
0.01893 |
0.01893 |
| L-Tryptophan |
0.0175 |
0.0175 |
| L-Tyrosine • 2Na • 2H2O |
0.0237 |
0.0237 |
| L-Valine |
0.025 |
0.025 |
| Vitamins |
| L-Ascorbic Acid |
0.05 |
0.05 |
| D-Biotin |
0.000025 |
0.000025 |
| Calciferol |
0.00025 |
0.00025 |
| Choline Chloride |
0.00125 |
0.00125 |
| Folic Acid |
0.000025 |
0.000025 |
| myo-Inositol |
0.000125 |
0.000125 |
| Menadione (sodium bisulfite) |
0.00004 |
0.00004 |
| Niacinamide |
0.0000625 |
0.0000625 |
| Nicotinic Acid |
0.0000625 |
0.0000625 |
| p-Aminobenzoic Acid |
0.000125 |
0.000125 |
| D-Pantothenic Acid (hemicalcium) |
0.000025 |
0.000025 |
| Pyridoxal • HCl |
0.0000625 |
0.0000625 |
| Pyridoxine • HCl |
0.0000625 |
0.0000625 |
| Retinol Acetate |
0.00025 |
0.00025 |
| Riboflavin |
0.000025 |
0.000025 |
| Thiamine • HCl |
0.000025 |
0.000025 |
| DL-α-Tocopherol Phosphate • 2Na |
0.000025 |
0.000025 |
| Vitamin B12 |
0.01 |
0.01 |
| Other |
| L-Amino-n-Butyric Acid |
0.00551 |
0.00551 |
| Cocarboxylase |
0.001 |
0.0001 |
| Coenzyme A • Na |
0.0025 |
0.0025 |
| 2'-Deoxyadenosine |
0.01 |
0.01 |
| 2'-Deoxycytidine • HCl |
0.01 |
0.01 |
| 2'-Deoxyguanosine • HCl |
0.01 |
0.01 |
| Flavin Adenine Dinucleotide • 2Na |
0.001 |
0.001 |
| D-Glucosamine • HCl |
0.00385 |
0.00385 |
| D-Glucose |
1 |
1 |
| Glucuronate • Na |
0.0018 |
0.0018 |
| D-Glucuronolactone |
0.0018 |
0.0018 |
| Glutathione • Na |
0.02 |
0.02 |
| 5'-Methylcytosine • HCl |
0.0001 |
0.0001 |
| β-NAD |
0.007 |
0.007 |
| β-NADP • Na |
0.001 |
0.001 |
| Phenol Red • Na |
0.02 |
0.02 |
| L-Taurine |
0.00418 |
0.00418 |
| Thymidine |
0.01 |
0.01 |
| TWEEN 80 |
0.0125 |
0.0125 |
| Uridine Triphosphate • Na |
0.001 |
0.001 |
| Add |
| L-Glutamine |
0.13573 |
— |
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