NCTC 109 Medium Formulation

Component	N1140 [1x] g/L	N3262 g/L
Inorganic Salts
Calcium Chloride	0.2	0.2
Magnesium Sulfate (anhydrous)	0.1	0.1
Potassium Chloride	0.4	0.4
Sodium Acetate (anhydrous)	0.03	0.03
Sodium Bicarbonate	2.2	—
Sodium Chloride	6.8	6.8
Sodium Phosphate Monobasic (anhydrous)	0.122	0.122
Amino Acids
L-Alanine	0.03148	0.03148
L-Arginine • HCl	0.03116	0.03116
L-Asparagine • H2O	0.00919	0.00919
L-Aspartic Acid	0.00991	0.00991
L-Cysteine • HCl • H2O	0.28971	—
L-Cystine • 2HCl	0.01368	0.01368
L-Glutamic Acid	0.00826	0.00826
L-Glutamine	—	0.13573
Glycine	0.01351	0.01351
L-Histidine • HCl • H2O	0.02665	0.02665
Hydroxy-L-Proline	0.00409	0.00409
L-Isoleucine	0.01804	0.01804
L-Leucine	0.02044	0.02044
L-Lysine • HCl	0.03843	0.03843
L-Methionine	0.00444	0.00444
L-Ornithine • HCl	0.00941	0.00941
L-Phenylalanine	0.01653	0.01653
L-Proline	0.00613	0.00613
L-Serine	0.01075	0.01075
L-Threonine	0.01893	0.01893
L-Tryptophan	0.0175	0.0175
L-Tyrosine • 2Na • 2H2O	0.0237	0.0237
L-Valine	0.025	0.025
Vitamins
L-Ascorbic Acid	0.05	0.05
D-Biotin	0.000025	0.000025
Calciferol	0.00025	0.00025
Choline Chloride	0.00125	0.00125
Folic Acid	0.000025	0.000025
myo-Inositol	0.000125	0.000125
Menadione (sodium bisulfite)	0.00004	0.00004
Niacinamide	0.0000625	0.0000625
Nicotinic Acid	0.0000625	0.0000625
p-Aminobenzoic Acid	0.000125	0.000125
D-Pantothenic Acid (hemicalcium)	0.000025	0.000025
Pyridoxal • HCl	0.0000625	0.0000625
Pyridoxine • HCl	0.0000625	0.0000625
Retinol Acetate	0.00025	0.00025
Riboflavin	0.000025	0.000025
Thiamine • HCl	0.000025	0.000025
DL-α-Tocopherol Phosphate • 2Na	0.000025	0.000025
Vitamin B12	0.01	0.01
Other
L-Amino-n-Butyric Acid	0.00551	0.00551
Cocarboxylase	0.001	0.0001
Coenzyme A • Na	0.0025	0.0025
2'-Deoxyadenosine	0.01	0.01
2'-Deoxycytidine • HCl	0.01	0.01
2'-Deoxyguanosine • HCl	0.01	0.01
Flavin Adenine Dinucleotide • 2Na	0.001	0.001
D-Glucosamine • HCl	0.00385	0.00385
D-Glucose	1	1
Glucuronate • Na	0.0018	0.0018
D-Glucuronolactone	0.0018	0.0018
Glutathione • Na	0.02	0.02
5'-Methylcytosine • HCl	0.0001	0.0001
β-NAD	0.007	0.007
β-NADP • Na	0.001	0.001
Phenol Red • Na	0.02	0.02
L-Taurine	0.00418	0.00418
Thymidine	0.01	0.01
TWEEN 80	0.0125	0.0125
Uridine Triphosphate • Na	0.001	0.001
Add
L-Glutamine	0.13573	—
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