Cell Culture

RPMI-1640 Media Formulation

RPMI-1640 Medium

Component R0883
[1x] g/L
R1145
[10x] g/L
R1383
g/L
R1780
[1x] g/L
R2405
[1x] g/L
R6504
g/L
R8758
[1x] g/L
Inorganic Salts
Calcium Nitrate • 4H2O 0.1 1 0.1 0.1 0.1 0.1 0.1
Magnesium Sulfate (anhydrous) 0.04884 0.4884 0.04884 0.04884 0.04884 0.04884 0.04884
Potassium Chloride 0.4 4 0.4 0.4 0.4 0.4 0.4
Sodium Bicarbonate 2 2 2 2
Sodium Chloride 6 60 6 6 6 6 6
Sodium Phosphate Dibasic (anhydrous) 0.8 8 0.8 0.8 0.8 0.8 0.8
Amino Acids
L-Alanyl-L-Glutamine 0.4344
L-Arginine 0.2 2 0.2 0.2 0.2 0.2
L-Asparagine (anhydrous) 0.05 0.5 0.05 0.05 0.05 0.05 0.05
L-Aspartic Acid 0.02 0.2 0.02 0.02 0.02 0.02 0.02
L-Cystine • 2HCl 0.0652 0.652 0.0652 0.0652 0.0652 0.0652 0.0652
L-Glutamic Acid 0.02 0.2 0.02 0.02 0.02 0.02 0.02
L-Glutamine 0.3 0.3 0.3 0.3
Glycine 0.01 0.1 0.01 0.01 0.01 0.01 0.01
L-Histidine 0.015 0.15 0.015 0.015 0.015 0.015 0.015
Hydroxy-L-Proline 0.02 0.2 0.02 0.02 0.02 0.02 0.02
L-Isoleucine 0.05 0.5 0.05 0.05 0.05 0.05 0.05
L-Leucine 0.05 0.5 0.05 0.05 0.05 0.05
L-Lysine • HCl 0.04 0.4 0.04 0.04 0.04 0.04
L-Methionine 0.015 0.15 0.015 0.015 0.015 0.015 0.015
L-Phenylalanine 0.015 0.15 0.015 0.015 0.015 0.015 0.015
L-Proline 0.02 0.2 0.02 0.02 0.02 0.02 0.02
L-Serine 0.03 0.3 0.03 0.03 0.03 0.03 0.03
L-Threonine 0.02 0.2 0.02 0.02 0.02 0.02 0.02
L-Tryptophan 0.005 0.05 0.005 0.005 0.005 0.005 0.005
L-Tyrosine • 2Na • 2H2O 0.02883 0.2883 0.02883 0.02883 0.02883 0.02883 0.02883
L-Valine 0.02 0.2 0.02 0.02 0.02 0.02 0.02
Vitamins
D-Biotin 0.0002 0.002 0.0002 0.002 0.002 0.0002 0.0002
Choline Chloride 0.003 0.03 0.003 0.003 0.003 0.003 0.003
Folic Acid 0.001 0.001 0.001 0.001 0.001 0.001
myo-Inositol 0.035 0.35 0.035 0.035 0.035 0.035 0.035
Niacinamide 0.001 0.01 0.001 0.001 0.001 0.001 0.001
p-Aminobenzoic Acid 0.001 0.01 0.001 0.001 0.001 0.001 0.001
D-Pantothenic Acid (hemicalcium) 0.00025 0.0025 0.00025 0.00025 0.00025 0.00025 0.00025
Pyridoxine • HCl 0.001 0.01 0.001 0.001 0.001 0.001 0.001
Riboflavin 0.0002 0.002 0.0002 0.0002 0.0002 0.0002 0.0002
Thiamine • HCl 0.001 0.01 0.001 0.001 0.001 0.001 0.001
Vitamin B12 0.000005 0.00005 0.000005 0.000005 0.000005 0.000005 0.000005
Other
D-Glucose 2 20 2 2 2 2
Glutathione (reduced) 0.001 0.01 0.001 0.001 0.001 0.001 0.001
Phenol Red • Na 0.0053 0.053 0.0053 0.0053 0.0053 0.0053
Add
L-Glutamine 0.3 0.3 at 1×
Sodium Bicarbonate 2.0 at 1× 2 2
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RPMI-1640 Medium Dutch Modification

Component R7638
[1x] g/L
Inorganic Salts
Calcium Nitrate • 4H2O 0.1
Magnesium Sulfate (anhydrous) 0.04884
Potassium Chloride 0.4
Sodium Bicarbonate 1
Sodium Chloride 6.4
Sodium Phosphate Dibasic (anhydrous) 0.8
Amino Acids
L-Arginine 0.2
L-Asparagine (anhydrous) 0.05
L-Aspartic Acid 0.02
L-Cystine • 2HCl 0.0652
L-Glutamic Acid 0.02
Glycine 0.01
L-Histidine 0.015
Hydroxy-L-Proline 0.02
L-Isoleucine 0.05
L-Leucine 0.05
L-Lysine • HCl 0.04
L-Methionine 0.015
L-Phenylalanine 0.015
L-Proline 0.02
L-Serine 0.03
L-Threonine 0.02
L-Tryptophan 0.005
L-Tyrosine • 2Na • 2H2O 0.02883
L-Valine 0.02
Vitamins
D-Biotin 0.0002
Choline Chloride 0.003
Folic Acid 0.001
myo-Inositol 0.035
Niacinamide 0.001
p-Aminobenzoic Acid 0.001
D-Pantothenic Acid (hemicalcium) 0.00025
Pyridoxine • HCl 0.001
Riboflavin 0.0002
Thiamine • HCl 0.001
Vitamin B12 0.000005
Other
D-Glucose 2
Glutathione (reduced) 0.001
HEPES 4.77
Phenol Red • Na 0.0053
Add
L-Glutamine 0.3
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RPMI-1640 Medium HEPES Modification

Component R4130
g/L
R5886
[1x] g/L
R8005
g/L
Inorganic Salts
Calcium Nitrate • 4H2O 0.1 0.1 0.1
Magnesium Sulfate (anhydrous) 0.04884 0.04884 0.04884
Potassium Chloride 0.4 0.4 0.4
Sodium Bicarbonate 2
Sodium Chloride 6 6 5.9
Sodium Phosphate Dibasic (anhydrous) 0.8 0.8 0.8
Amino Acids
L-Arginine 0.2 0.2 0.2
L-Asparagine (anhydrous) 0.05 0.05 0.05
L-Aspartic Acid 0.02 0.02 0.02
L-Cystine • 2HCl 0.0652 0.0652 0.0652
L-Glutamic Acid 0.02 0.02 0.02
L-Glutamine 0.3 0.3
Glycine 0.01 0.01 0.01
L-Histidine 0.015 0.015 0.015
Hydroxy-L-Proline 0.02 0.02 0.02
L-Isoleucine 0.05 0.05 0.05
L-Leucine 0.05 0.05 0.05
L-Lysine • HCl 0.04 0.04 0.04
L-Methionine 0.015 0.015 0.015
L-Phenylalanine 0.015 0.015 0.015
L-Proline 0.02 0.02 0.02
L-Serine 0.03 0.03 0.03
L-Threonine 0.02 0.02 0.02
L-Tryptophan 0.005 0.005 0.005
L-Tyrosine • 2Na • 2H2O 0.02883 0.02883 0.02883
L-Valine 0.02 0.02 0.02
Vitamins
D-Biotin 0.0002 0.0002 0.0002
Choline Chloride 0.003 0.003 0.003
Folic Acid 0.001 0.001 0.001
myo-Inositol 0.035 0.035 0.035
Niacinamide 0.001 0.001 0.001
p-Aminobenzoic Acid 0.001 0.001 0.001
D-Pantothenic Acid (hemicalcium) 0.00025 0.00025 0.00025
Pyridoxine • HCl 0.001 0.001 0.001
Riboflavin 0.0002 0.0002 0.0002
Thiamine • HCl 0.001 0.001 0.001
Vitamin B12 0.000005 0.000005 0.000005
Other
D-Glucose 2 2 4.5
Glutathione (reduced) 0.001 0.001 0.001
HEPES 5.96 5.96 3.5745
Phenol Red • Na 0.0053 0.0053 0.0053
Add
L-Glutamine 0.3
Sodium Bicarbonate 2 2
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RPMI-1640 Medium Modified

Component R7388
[1x] g/L
R7509
[1x] g/L
R7513
[1x] g/L
R8755
g/L
Inorganic Salts
Calcium Nitrate • 4H2O 0.1 0.1 0.1 0.1
Magnesium Sulfate (anhydrous) 0.04884 0.04884 0.04884 0.04884
Potassium Chloride 0.4 0.4 0.4 0.4
Sodium Bicarbonate 2 2
Sodium Chloride 6 6 6 6
Sodium Phosphate Dibasic (anhydrous) 0.8 0.8 0.8 0.8
Amino Acids
L-Arginine 0.2 0.2 0.2 0.2
L-Asparagine (anhydrous) 0.05 0.05 0.05 0.05
L-Aspartic Acid 0.02 0.02 0.02 0.02
L-Cystine • 2HCl 0.0652 0.0652 0.0652
L-Glutamic Acid 0.02 0.02 0.02 0.02
L-Glutamine 0.3 0.3
Glycine 0.01 0.01 0.01 0.01
L-Histidine 0.015 0.015 0.015 0.015
Hydroxy-L-Proline 0.02 0.02 0.02 0.02
L-Isoleucine 0.05 0.05 0.05 0.05
L-Leucine 0.05 0.05 0.05 0.05
L-Lysine • HCl 0.04 0.04 0.04 0.04
L-Methionine 0.015 0.015 0.015
L-Phenylalanine 0.015 0.015 0.015 0.015
L-Proline 0.02 0.02 0.02 0.02
L-Serine 0.03 0.03 0.03 0.03
L-Threonine 0.02 0.02 0.02 0.02
L-Tryptophan 0.005 0.005 0.005 0.005
L-Tyrosine • 2Na • 2H2O 0.02883 0.02883 0.02883 0.02883
L-Valine 0.02 0.02 0.02 0.02
Vitamins
D-Biotin 0.0002 0.0002 0.0002 0.0002
Choline Chloride 0.003 0.003 0.003 0.003
Folic Acid 0.001 0.001 0.001 0.001
myo-Inositol 0.035 0.035 0.035 0.035
Niacinamide 0.001 0.001 0.001 0.001
p-Aminobenzoic Acid 0.001 0.001 0.001 0.001
D-Pantothenic Acid (hemicalcium) 0.00025 0.00025 0.00025 0.00025
Pyridoxine • HCl 0.001 0.001 0.001 0.001
Riboflavin 0.0002 0.0002 0.0002 0.0002
Thiamine • HCl 0.001 0.001 0.001 0.001
Vitamin B12 0.000005 0.000005 0.000005 0.000005
Other
D-Glucose 2 2 2 2
Glutathione (reduced) 0.001 0.001 0.001 0.001
HEPES 4.77
Phenol Red • Na 0.0053 0.0053
Add
L-Cystine • 2HCl 0.0652
L-Glutamine 0.3 0.3
L-Methionine 0.015
Sodium Bicarbonate 2 2
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RPMI-1640 Medium Auto-Mod™ Modified for Autoclaving

Component R7755
g/L
Inorganic Salts
Calcium Nitrate • 4H2O 0.1
Magnesium Sulfate (anhydrous) 0.04884
Potassium Chloride 0.4
Sodium Chloride 6
Sodium Phosphate Dibasic (anhydrous) 0.8
Succinic Acid • 6H2O • Na 0.1
Succinic Acid (free acid) 0.075
Amino Acids
L-Arginine 0.2
L-Asparagine (anhydrous) 0.05
L-Aspartic Acid 0.02
L-Cystine • 2HCl 0.0652
L-Glutamic Acid 0.02
Glycine 0.01
L-Histidine 0.015
Hydroxy-L-Proline 0.02
L-Isoleucine 0.05
L-Leucine 0.05
L-Lysine • HCl 0.04
L-Methionine 0.015
L-Phenylalanine 0.015
L-Proline 0.02
L-Serine 0.03
L-Threonine 0.02
L-Tryptophan 0.005
L-Tyrosine 0.02
L-Valine 0.02
Vitamins
D-Biotin 0.0002
Choline Bitartrate 0.00544
Folic Acid 0.001
myo-Inositol 0.035
Niacinamide 0.001
p-Aminobenzoic Acid 0.001
D-Pantothenic Acid (hemicalcium) 0.00025
Pyridoxine • HCl 0.001
Riboflavin 0.0002
Thiamine • HCl 0.001
Vitamin B12 0.000005
Other
D-Glucose 2
Glutathione (reduced) 0.001
Phenol Red • Na 0.00318
Add
L-Glutamine 0.3
Sodium Bicarbonate 2
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