Williams' Medium E Formulation

Component	W1878 [1x] g/L	W4125 g/L	W4128 [1x] g/L
Inorganic Salts
Calcium Chloride	0.2	0.2	0.2
Cupric Sulfate • 5H2O	0.0000001	0.0000001	0.0000001
Ferric Nitrate • 9H2O	0.0000001	0.0000001	0.0000001
Magnesium Chloride • 4H2O	0.0000001	0.0000001	0.0000001
Magnesium Sulfate (anhydrous)	0.0977	0.0977	0.0977
Potassium Chloride	0.4	0.4	0.4
Sodium Bicarbonate	2.2	—	2.2
Sodium Chloride	6.8	6.8	6.8
Sodium Phosphate Monobasic (anhydrous)	0.122	0.122	0.122
Zinc Sulfate • 7H2O	0.0000002	0.0000002	0.0000002
Amino Acids
L-Alanine	0.09	0.09	0.09
L-Arginine (free base)	0.05	0.05	0.05
L-Asparagine • H2O	0.02	0.02	0.02
L-Aspartic Acid	0.03	0.03	0.03
L-Cysteine (free acid)	0.04	0.04	0.04
L-Cystine	0.02	0.02	0.02
L-Glutamic Acid	0.0445	0.0445	0.0445
L-Glutamine	—	0.292	—
Glycine	0.05	0.05	0.05
L-Histidine (free base)	0.015	0.015	0.015
L-Isoleucine	0.05	0.05	0.05
L-Leucine	0.075	0.075	0.075
L-Lysine • HCl	0.08746	0.08746	0.08746
L-Methionine	0.015	0.015	0.015
L-Phenylalanine	0.025	0.025	0.025
L-Proline	0.03	0.03	0.03
L-Serine	0.01	0.01	0.01
L-Threonine	0.04	0.04	0.04
L-Tryptophan	0.01	0.01	0.01
L-Tyrosine • 2Na • 2H2O	0.05045	0.05045	0.05045
L-Valine	0.05	0.05	0.05
Vitamins
Ascorbic Acid • Na	0.00227	0.00227	0.00227
D-Biotin	0.0005	0.0005	0.0005
Calciferol	0.0001	0.0001	0.0001
Choline Chloride	0.0015	0.0015	0.0015
Folic Acid	0.001	0.001	0.001
myo-Inositol	0.002	0.002	0.002
Menadione (sodium bisulfite)	0.00001	0.00001	0.00001
Niacinamide	0.001	0.001	0.001
D-Pantothenic Acid (hemicalcium)	0.001	0.001	0.001
Pyridoxal • HCl	0.001	0.001	0.001
Retinol Acetate	0.0001	0.0001	0.0001
Riboflavin	0.0001	0.0001	0.0001
Thiamine • HCl	0.001	0.001	0.001
DL-α-Tocopherol Phosphate • Na	0.00001	0.00001	0.00001
Vitamin B12	0.002	0.0002	0.0002
Other
D-Glucose	2	2	2
Glutathione (reduced)	0.00005	0.00005	0.00005
Methyl Linoleate	0.00003	0.00003	0.00003
Phenol Red • Na	—	0.0107	0.0107
Pyruvic Acid • Na	0.025	0.025	0.025
Add
L-Glutamine	0.292	—	0.292
Sodium Bicarbonate	—	2.2	—
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