CHO DHFR Medium

Get Maximum Results from Your CHO DHFR^- System with New CHO DHFR^- Medium, Animal Component-Free

(Product Code C 8862)

This latest addition to the growing line of animal component-free CHO media from Sigma-Aldrich has been designed to provide outstanding cell growth and recombinant protein productivity in Chinese hamster ovary (CHO) cells that are engineered to contain the dihydrofolate reductase (DHFR) amplification system. Contains recombinant human insulin, Pluronic® F-68, and plant-based hydrolysates

CHO DHFR^- Medium provides the following benefits:

• Animal Component-free — contains no transferrin or other products of animal origin
• Designed for DHFR^- CHO clones — no hypoxanthine or thymidine
• Outperforms the competition in terms of cell growth and productivity
• Supports proper glycosylation of recombinant proteins
• Exceptional performance in other selection systems, too

Life Science Quarterly: Volume 4, Issue 3, Summer 2003

• Creating a New Medium to Meet the Variable Nutritional Requirements of Chinese Hamster Ovary (CHO) Cell Clones

   

  
  

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  Figures 1 & 2: Cell growth and recombinant protein productivity in two CHO DFHR^- clones were compared under suspension conditions in C 8862 and in animal component-free media from two competitors. Samples were taken daily to analyze cell growth and recombinant protein productivity. The data indicates that C 8862 consistently supports the highest cell densities and recombinant protein productivity.

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