Cell Culture

Embryo Culture Media

Today, transgenic techniques are employed with a wide range of organisms to study gene function. Transgenic mice are used extensively in molecular biology for the study of gene function, because they are relatively easy to work with and inexpensive. One widely used technique for creating transgenic mice involves the creation and injection of stably transfected mouse embryonic stem cells (ES) into mouse blastocyst embryos.

Application Note: Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

To enable embryo collection, manipulation, and transfer techniques, we offer a wide selection of mouse embryo media and reagents including M2, modified M16, FHM and proprietary KSOM mouse embryo media formulations. All of our media are tested on mouse embryos and manufactured using the highest quality raw materials available. Both liquid and powder EmbryoMax™ media are available for many classic formulations.

Embryo Handling and Manipulation (HEPES-based media formulations)

M2: M2 embryo media is a modified Krebs-Ringer solution buffered with HEPES, is commonly used for collection of embryos and when handling them outside the incubator for extended periods of time.

FHM: With a function similar to M2, the FHM medium is a modification of KSOM where part of bicarbonate is replaced with HEPES buffer. FHM is typically used as an embryo medium for washing and handling embryos outside the CO2 incubators.

Embryo Culture and Development (Bicarbonate-based formulations)

KSOM: KSOM media allows outbred zygotes to overcome the two-cell block and supports in vitro and in vivo Development of various mouse strains. KSOM allows for higher rates of cell division and produces higher yields of blastocyst development.

M16: Classic embryo culture media based on modified Krebs-Ringer bicarbonate solution.


Stages of Embryo Development

Stages of Embryo Development

 

New Product Focus

EmbryoMax® Advanced KSOM Embryo Medium

Advanced KSOM is a new uniquely buffered modified version of the original KSOM formulation that enables:
  • One medium for both embryo handling in atmospheric conditions and culturing in a CO2 Incubator.
  • Higher frequency of blastocyst formation across multiple mice strains
  • No medium pre-equilibration step required
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  Mouse embryo development in KSOM vs advanced KSOM

Mouse Embryo Media

Part Number Description
M7292 M16 Medium With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered
MR-010P EmbryoMax® Modified M16 Medium (1X), Powdered, without phenol red, 5 x 10ml
M7167 M2 medium With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo
MR-015P EmbryoMax® M2 Medium (1X), Powdered, with phenol red
MR-106 EmbryoMax® KSOM Medium (1X) w/ 1/2 Amino Acids
MR-121 EmbryoMax® KSOM Medium (1X) w/ 1/2 Amino Acids & Phenol Red
MR-107 EmbryoMax® KSOM Medium (1X) w/ 1/2 Amino Acids w/o BSA
MR-020P EmbryoMax® KSOM Embryo Culture (1X), Powder, w/o Phenol Red, 250ml
MR-070 EmbryoMax® Human Tubal Fluid (HTF) (1X), liquid, for Mouse IVF
T1788 Tyrode’s Solution, Acidic liquid, sterile-filtered, suitable for mouse embryo
MR-019 EmbryoMax® CZB Medium With Phenol Red
MR-024 EmbryoMax® FHM HEPES Buffered Medium (1X), liquid, w/ Phenol Red

 

Mouse Embryo Tested Reagents

Part Number Description
A3311 Bovine Serum Albumin powder, BioXtra
C8554 Chorionic gonadotropin human lyophilized powder, mouse embryo tested
G6152 D-(+)-Glucose suitable for mouse embryo, ≥99.5% (GC)
H6147 HEPES BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)
H4272 Hyaluronidase from bovine testes Type IV-S, powder, mouse embryo tested, 750-3000 units/mg solid
M8410 Mineral oil light oil (neat), BioReagent, suitable for mouse embryo cell culture
M5310 Mineral oil light oil ((neat)), BioXtra, suitable for mouse embryo cell culture
P0930 Polyvinylpyrrolidone powder, BioXtra, suitable for mouse embryo
P8811 Protease from Streptomyces griseus powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid
S8776 Sodium chloride solution (0.9%), BioXtra, suitable for cell culture
L7900 Sodium DL-lactate solution synthetic, syrup, BioXtra, suitable for mouse embryo cell culture, 60 % (w/w)
P4562 Sodium pyruvate powder, BioXtra, suitable for mouse embryo
W1503 Water for embryo transfer, sterile-filtered, BioXtra, suitable for mouse embryo
ES-006 EmbryoMax® 0.1% Gelatin Solution
BSS-1006 EmbryoMax® 1X Dulbecco's Phosphate Buffered Saline w/o Ca++ & Mg++
ES-003 EmbryoMax® Electroporation Buffer