The Cell Design Studio (CDS) group at Sigma-Aldrich® leverages several molecular biology technologies, most notably Custom shRNA and CompoZr® Zinc Finger Nuclease Technology, to engineer mammalian lines for customers to use in areas such as basic research, cell-based assays, target validation, drug discovery and bioproduction. Our mission is to facilitate customers research and manufacturing needs through application of our experience in cell engineering technologies.
Zinc Finger Nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. Double-strand breaks are important for site-specific mutagenesis in that they stimulate the cell's natural DNA-repair processes, namely homologous recombination and Non-Homologous End Joining (NHEJ). By implementing established, field-proven methods, these processes can be harnessed to generate precisely targeted genomic edits, resulting in cell lines with precise and heritable gene deletions, integrations or modifications.
Combining ZFN technology and stem cell expertise, Sigma-Aldrich now offers a service for the custom engineering of induced pluripotent stem (iPS) cells. Send us your iPS cells or use one of our well characterized iPS cell lines and get a fit-for-your-purpose ready-to-use cell line. Potential projects include, but are not limited to, creation of pluripotency reporter, lineage tagged, or disease model iPS cell lines. Let Sigma-Aldrich iPS CDS team focus on development and spend your precious time and resources on discovery.
Engage our team to discuss your specific needs by sending a brief email to CDSInquiries@sial.com to connect with a CDS representative. Projects can be initiated by completion of a Cell Design Studio Application Form provided by the CDS representative and subsequent acceptance of a Statement of Work prepared by the CDS team.
To facilitate our clients’ research and manufacturing endeavors through expert application and support of Sigma-Aldrich cell engineering technologies.
Why use our Cell Design Studio (CDS)?
Applications of Cell Lines from CDS
Our Project Workflow