Custom Engineered Cell Lines

Cell Design Studio

 Custom Engineered Cells from Sigma® Life Sciences

The Cell Design Studio team at Sigma Aldrich is made up of experienced and dedicated scientists that work to provide a solution to your questions. With in-house access to CRISPR, ZFN, and shRNA technologies, the generation of gene knockouts, knockins and gene corrections in your model cell line is both rapid and efficient. We have invested the time and energy into optimizing the workflow for custom cell line engineering from the bioinformatic design of the nuclease to the development of detection assays needed to identify a potentially rare event in a pool of cells.  Partner with us for your custom engineering needs while you focus on research.

 Project Types


Zinc Finger Nuclease, CRISPR and shRNA technologies are utilized to modify your gene of interest. Learn more about each project type below.


 How Projects are Executed

Our project initiation process, technical evaluation step, and distinct phases of execution allow for smooth delivery of each project from start to finish. Learn more about our workflow here.

Projects can be executed in full or nearly any portion of a project can be executed upon separately based on your needs.

 Getting a Project Started

Reach out to us through the “Contact Us” button below. You will be contacted by a member of the Cell Design Studio team to discuss technical aspects of your project or answer any questions you may have.


 As Your Solutions Provider, We Ask You the Right Questions

Your cell line needs to be a solution. Because of our years of experience and dedicated scientists, we are able to work with you in an open and collaborative manner to be sure the cell line we deliver is a solution not simply a product.

  • Solutions based approach to engineering
  • Easy access to CompoZr ZFN Technology, Sigma CRISPRs and MissionRNAi
  • Rapid delivery of your modified cell lines
  • Dedicated and Experienced cell design R&D team will partner with you to create your cell line
  • Managed projects so that time lines and deliverables are prioritized and met


 iPS Cell Capabilities

  • Genetic modification of iPS Cells
  • Generation of iPS Cells
  • Measurement of pluripotent and differentiation markers
  • In vitro lineage differentiation

 Assay Options

Additional assays can be run on your modified cell line after engineering is complete. Assays can also be run outside the scope of an engineering project on your cell line of interest.


Droplet Digital PCR
  • Copy number determination
  • Rare event detection
  • Next-generation sequencing validation
  • Gene expression studies


Phenotypic Screening (PDF)
  • Compound sensitivity
  • Expression profiling, induction/repression


Cytometric Analysis
  • Fluorescent protein analysis
  • Cell sorting of target populations
  • Multi-color cell phenotyping


Cell Characterization

  • Off-target screening
  • mRNA detection
  • Protein analysis
  • Receptor expression analysis


To utilize our custom assay services, please e-mail us at

Our capabilities don’t stop here. No matter how obscure or exact your project requirements are, let our experts assist in simplifying your custom project.

 What Our Customers Have to Say

"This program involved the transfection of Sigma’s AC2 cell line with a codon optimized gene vector, the selection of single cell clones expressing a viral protein, the transfer and establishment of a FACS based assay for gene expression, the characterization of the levels of expression of the protein in hundreds of clones, as well as the characterization of cell growth characteristics for these clones."

"We were extremely impressed by the thoughtful and efficient manner in which Sigma developed the Statement of Work."

"Once the contract was in place, work proceeded very efficiently. Interactions with our internal team and Sigma were highly professional, and Sigma was very responsive to our suggestions and requirements."

— Thomas P. Monath MD, Hookipa BioTech AG

 Meet Our Staff of Highly Trained Scientists

Your project couldn’t be in more capable hands. Learn more about our scientists here.

When asked, “What do you enjoy about working on the Cell Design Studio team?”

“I enjoy the challenge that every new project brings and I value the collaborative professional culture. When faced with something particularly challenging the team works together to find a solution. We are not just delivering a product; we are developing new tools and solutions and always pushing the envelope with creative new ideas and approaches.”

— Suzanne Hibbs, Scientist, Cell Design Studio