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Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.


Custom Project Flexibility



 Cell Lines and Scalability

Cell Design studio has engineered a breadth of mammalian cell lines using both CRISPR and ZFN technologies.  We can provide an in-house cell line or use your existing cell line to best meet your needs. In addition to immortalized lines, we have substantial experience working with human iPSCs and ESCs, and offer stem cell-specific services such as pluripotency testing and differentiation. We are also able to deliver across a wide range of scales, whether you need a dozen individual clones of 106 cells each, or one clone of 1010 cells.

Select examples of cell lines engineered by Cell Design Studio

5TGM1 786-O A20 A549 C2BBe1 Caco-2 CHO-K1 CT26 DLD-1  
GM12878 GM24385 H838 HCT116 HEK293 hESC hiPSC HepaRG HepG2 ID8
Jurkat K562 Kit225 LNCaPs MCF7 MCF10a P3x63Ag8 Pan02 RAW264 RKO
RKO-E6 SH-SY5Y SKOV3 SW-48 T84 THP-1 U2OS WS1    

 

Select examples of genes targeted by Cell Design Studio

4EBP1 ABL Actin AKT1 AKT2 APC ATM BARD1 BAX BAK
β-catenin BCR BCRP Brachyury B-raf BRCA1 BRCA2 BRIP1 CAV1 CDC2
CDC25B CDC25C CDH1 CFTR CHK1 CHK2 CK1a CLDN6 CNP CSMD1
CTNNB1 CyclinB1 DICER1 eEF2K EGFR ERBB3 ERBB4 ESR1 ESR2 FBX15
FG1B FGFR4 FLT1 FLT3 GalC GATA4 GATA6 GFAP GRIA2 GSK3B
H2AFX HCN4 HER2 HEY1 HEY2 HMG HMGA1 IGF1R INK4a ISL1
MDR1 MET MRP2 mTOR MXL1 MYBPC3 MYCBP2 MYH6 MYL2 MYL7
Myosin Nestin NKX2.1 NKX2.5 NRK NUP98 OCT4 Olig2 P53 p70s6K
PARP1 PARP2 Pax6 PDX1 PEA15 PIK3CA PIK3CD PLP PTEN PTPRJ
PXR RAD51 RB1 Rictor RSK1 SERPIN1 SFTPC SMAD3 SMAD4 SMO
SNAIL1 SNAIL2 Sox2 STAT1 STAT2 STAT3 SV2A SYK TEK TGFB1
TH TNNT2 Top1 TOP2A TP53 TRAILR2 TUBB3 UCHL1 VEGFR1 VEGFR2
VEGFR3 VIM WEE1 Wnt3a Wnt5a WWTR1        

 

 CDS Express

At Cell Design Studio, we take pride in meeting our customers’ needs, including tight timelines and limited budgets. In response to these frequent project requirements, we are excited to introduce CDS Express services. CDS Express projects are carried out on select immortalized cell lines or a Sigma in-house iPS cell line using pre-validated zinc finger nucleases or CRISPR gRNAs. Cells are delivered to you as a bulk modified pool verified to possess the modification of interest - allowing you to single cell clone or use the cells as a heterogeneous pool, depending on your needs. By using validated nucleases in lines with which we have substantial experience, we can offer CDS Express services to you at a reduced price with a reduced timeline to completion relative to full-service projects. And thanks to Sigma-Aldrich’s quality control practices, you can count on CDS Express to meet your needs just as much as a full-service project.

CDS Express Cell Lines

A549 C2BBe1 HEK293 Hep2G iPSC*
Jurkat K562 SH-SY5Y THP-1** U2OS

*Sigma in-house iPSC line
**Available for knockout only
 

 Post-modification Services

Cell Design Studio offers an expansive suite of services to provide a comprehensive, end-to-end solution for your scientific questions. We work with you to deliver not only the cells you need, but also the information you need. Cell characterization, population analysis, expression and reporter analysis, and phenotyping services are all available. Contact Us Today to see what we can do to expedite your research goals.
 

Cell characterization Copy number determination; screening for off-target nuclease activity; detection of random integration events; mRNA detection; protein analysis; cell surface receptor expression analysis; authentication of cell line identity; verification/validation of engineered change
Off-target screening and detection In silico screening of off-target nuclease activity; PCR/Sanger sequencing verification of top potential off-target sites; whole exome sequencing; whole genome sequencing
Population analysis: flow cytometry analysis Multi-colored assay development; population analysis; cell sorting services
Expression analysis: reporter assays Luminescence assays; fluorescence assays; time-resolved fluorescence; fluorescence polarization
Phenotypic analysis and screening: imaging Immunofluorescence (immunostaining); proximity assays (Duolink, FRET); live cell video imaging; ratiometric assays; redistribution assays; small-scale compound screening; HCS assay development
Biobanking services Scale-up and banking of cell lines (immortalized and stem cells)