Biological Buffers

Bicine

Synonym: N,N-Bis(2-hydroxyethyl)glycine
 

Bicine is a zwitterionic buffer that is recommended for low temperature biochemical work1. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published2. Bicine has been utilized in peptide and protein crystallization3,4,5. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described6.
Useful pH Range 7.6 - 9.0
Working concentration range 40mM-100mM9,10

 

        Comparison of products by grade or quality level
Name CAS Molecular Formula Mol Wt Reagent Grades Life Science Grades PharmaGrade  Solution
Bicine 150-25-4 C6H13NO4 163.17 B3876 391336-M B8660 RES1151B-B7   

 

Interaction Comments
 

Bicine interferes with the BCA and Lowry protein assay7.
Ferricyanide slowly oxidizes bicine7.
Bicine strongly interacts with Mg, Ca, Fe, Co, Ni, Cu, Zn and Cd. There is slight interaction with Mn8

 

Literature References

  1. Good, N. E., et al, Hydrogen ion buffers for biological research. Biochemistry, 5(2), 467-477 (1966).
  2. Luo, Q., et al., Thin-layer ion-exchange chromatography of proteins. J. Chromatogr. A, 816(1), 97-105 (1998).
  3. Kanyo, Z. F., et al., Crystallization and oligomeric structure of rat liver arginase. J. Mol. Biol., 224(4), 1175-1177 (1992).
  4. Watanabe, L., et al., Crystallization and preliminary diffraction data of BaP1, a haemorrhagic metalloproteinase from Bothrops asper snake venom. Acta Crystallogr. D Biol. Crystallogr., 58(Pt 6 Pt 2), 1034-1035 (2002).
  5. Abad, M. C, et al., The X-ray crystallographic structure of the angiogenesis inhibitor angiostatin. J. Mol. Biol., 318(4), 1009-1017 (2002).
  6. Wiltfang, J., et al., A new multiphasic buffer system for sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins and peptides with molecular masses 100,000-1000, and their detection with picomolar sensitivity. Electrophoresis, 12(5), 352-366 (1991).
  7. Bollag, D.M. & Edelstein, S.J. (1992) Protein Methods. Chapter 1, II. Wiley-Liss. New York
  8. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003
  9. Garrett RH, Grisham CM Biochemistry. Brooks/Cole, 2013 pp.50
  10. Budin I, Debnath A, Szostak JW, Concentration-Driven Growth of Model Protocell Membranes, J Am Chem Soc. 2012 Dec 26; 134(51): 20812–20819.