Biological Buffers

BIS-TRIS Propane

BIS-TRIS propane is a zwitterionic buffer with an unusually wide buffering range, from approximately pH 6 to 9.5, because its two pKa values are close in value. BIS-TRIS propane has been used to enhance the stability or activity of restriction enzymes, compared to Tris buffer1.
Useful pH Range 6.3 - 9.5
Working concentration range 10mM-50mM4,5

 

        Comparison of products by grade or quality level
Name CAS Molecular Formula Mol Wt Reagent Grades Life Science Grades PharmaGrade  Solution
BIS-TRIS propane 64431-96-5 C11H26N2O6 282.33 RDD014 B6755 B4910 B4679 PHG0005  

 

Interaction Comments
 

Strong interaction with Ni, Co, Cu, Zn, Cd and Pb3

References

  1. Eckert, K. A., and Kunkel, T. A., DNA polymerase fidelity and the polymerase chain reaction. PCR Methods Appl., 1(1), 17-24 (1991).
  2. Dutta K, Natarajan A, Nair P, Shuman S, and Ghose R. “Sequence-specific 1H, 13C and 15N assignments of the phosphoesterase (PE) domain of Pseudomonas aeruginosa DNA ligase D” Biomol. 2011 October.
  3. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003.
  4. Moldoveanu S, David V, Essentials in Modern HPLC separation, Elsevier 2013 pp. 413.
  5. Makarchikov AF, Brans A, Betterdorf FL, Thiamine diphosphate adenylyl transferase from E. coli: functional characterization of the enzyme synthesizing adenosine thiamine triphosphate, BMC Biochemistry 2007 V8 N17.