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Biological Buffers
BIS-TRIS
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 Printer friendly PDF version (390 Kb)
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| Sigma-Aldrich® BIS-TRIS Physical Properties |
| Synonyms |
2,2-Bis(hydroxymethyl)-2,2',2"-nitrilotriethanol
2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol
Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane |
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| Molecular Formula |
C8H19NO5 |
Beilstein Registry |
2205275 |
Useful pH range |
5.8 - 7.2 |
| Molecular Weight |
209.24 |
MDL Number |
MFCD00002853 |
pKa (at 25) |
6.5 |
| CAS Number |
6976-37-0 |
EG/EC Number |
230-237-7 |
pKa (at 37) |
6.36 |
| Sigma-Aldrich BIS-TRIS Specifications Comparison |
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B9754 |
B4429 |
B7535 |
14879 |
| Grade |
≥98.0% (titration) |
BioReagent, cell culture tested, suitable for insect cell culture,
≥98% (titration) |
BioXtra, ≥98.0% (titration) |
BioUltra, ≥99.0% (NT) |
| Purity |
≥98.0% |
≥98.0% |
>98.0% |
≥99.0% |
| Reagent Testing |
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B9754 |
B4429 |
B7535 |
14879 |
Enhanced Quality
Profile Buffers |
X |
X |
X |
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| 280NM |
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≤0.15 |
| IR |
X |
X |
X |
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| pH |
9.0 - 10.1 |
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9.0 - 11.0
(20 °C, 1 M in H2O) |
9.5 - 11.0
(25 °C, 1 M in H2O) |
| Karl Fischer |
≤1% |
≤1% |
≤1% |
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| LOD |
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≤0.5% |
| Insoluble Matter |
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<0.1% |
filter test |
| Residue on Ignition |
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<0.1% |
≤0.3% |
| Life Science Testing |
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B9754 |
B4429 |
B7535 |
14879 |
| Endotoxin |
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≤1 eu/mg |
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| SPC |
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≤100 cfu/g |
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| Cell test |
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X |
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| Insect cell test |
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X |
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| RNAse |
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X |
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| DNAse |
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X |
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| Nickase |
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X |
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| Protease |
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X |
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| Trace Elemental Impurities |
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B9754 |
B4429 |
B7535 |
14879 |
| Ammonia(NH4) |
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<0.05% |
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| Sodium(Na) |
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<0.005% |
≤0.005% |
| Phosphorus(P) |
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<0.005% |
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| Chloride(Cl) |
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<0.05% |
≤0.005% |
| Sulfate(SO4) |
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<0.05% |
≤0.005% |
| Aluminum(Al) |
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<0.0005% |
≤0.0005% |
| Arsenic(As) |
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≤0.00001% |
| Barium(Ba) |
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≤0.0005% |
| Bismuth(Bi) |
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≤0.0005% |
| Calcium(Ca) |
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<0.0005% |
≤0.001% |
| Cadmium(Cd) |
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≤0.0005% |
| Cobalt(Co) |
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≤0.0005% |
| Chromium(Cr) |
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≤0.0005% |
| Copper(Cu) |
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<0.0005% |
≤0.0005% |
| Iron(Fe) |
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<0.0005% |
≤0.0005% |
| Potassium(K) |
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<0.005% |
≤0.005% |
| Lithium(Li) |
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≤0.0005% |
| Magnesium(Mg) |
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<0.0005% |
≤0.0005% |
| Manganese(Mn) |
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≤0.0005% |
| Molybdenum(Mo) |
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≤0.0005% |
| Nickel(Ni) |
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≤0.0005% |
| Lead(Pb) |
≤0.0005% |
≤0.0005% |
<0.001% |
≤0.0005% |
| Strontium(Sr) |
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≤0.0005% |
| Zinc(Zn) |
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<0.0005% |
≤0.0005% |
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