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Biological Buffers

BIS-TRIS

BIS-TRIS Specification Comparison Printer friendly PDF version (101 Kb)

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Sigma-Aldrich® BIS-TRIS Physical Properties
Synonyms     2,2-Bis(hydroxymethyl)-2,2',2"-nitrilotriethanol
    2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol
    Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane		 BIS-TRIS Image
Molecular Formula C8H19NO5 Beilstein Registry 2205275 Useful pH range 5.8 - 7.2
Molecular Weight 209.24 MDL Number MFCD00002853 pKa (at 25) 6.5
CAS Number 6976-37-0 EG/EC Number 230-237-7 pKa (at 37) 6.36

Sigma-Aldrich BIS-TRIS Specifications Comparison
  B9754 B4429 B7535 14879
Grade ≥98.0% (titration) BPC, cell culture tested, insect cell culture tested,
≥98% (titration)		 SigmaUltra, ≥98.0% (titration) BioUltra, ≥99.0% (NT)
Purity ≥98.0% ≥98.0% >98.0% ≥99.0%
Reagent Testing
  B9754 B4429 B7535 14879
Enhanced Quality
Profile Buffers		 X      
280NM       ≤0.15
IR   X    
pH 5.8 - 7.2 5.8 - 7.2 9.0 - 11.0
(20 °C, 1 M in H2O) 		9.5 - 11.0
(25 °C, 1 M in H2O)
Karl Fischer ≤1% ≤1%    
LOD       ≤0.5%
Insoluble Matter     <0.1% filter test
Residue on Ignition     <0.1% ≤0.3%
Life Science Testing
  B9754 B4429 B7535 14879
Endotoxin   ≤1 eu/mg    
SPC   ≤100 cfu/g    
Cell test   X    
RNAse   X    
DNAse   X    
Nickase   X    
Protease   X    
Trace Elemental Impurities
  B9754 B4429 B7535 14879
Ammonia(NH4)     <0.05%  
Sodium(Na)     <0.005% ≤0.005%
Phosphorus(P)     <0.005%  
Chloride(Cl)     <0.05% ≤0.005%
Sulfate(SO4)     <0.05% ≤0.005%
Aluminum(Al)     <0.0005% ≤0.0005%
Arsenic(As)       ≤0.00001%
Barium(Ba)       ≤0.0005%
Bismuth(Bi)       ≤0.0005%
Calcium(Ca)     <0.0005% ≤0.001%
Cadmium(Cd)       ≤0.0005%
Cobalt(Co)       ≤0.0005%
Chromium(Cr)       ≤0.0005%
Copper(Cu)     <0.0005% ≤0.0005%
Iron(Fe)     <0.0005% ≤0.0005%
Potassium(K)     <0.005% ≤0.005%
Lithium(Li)       ≤0.0005%
Magnesium(Mg)     <0.0005% ≤0.0005%
Manganese(Mn)       ≤0.0005%
Molybdenum(Mo)       ≤0.0005%
Nickel(Ni)       ≤0.0005%
Lead(Pb) ≤0.0005% ≤0.0005% <0.001% ≤0.0005%
Strontium(Sr)       ≤0.0005%
Zinc(Zn)     <0.0005% ≤0.0005%
 
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