Biological Buffers

HEPES Sodium Salt

BIS-TRIS Specification Comparison Printer friendly PDF version (408 Kb)
Sigma-Aldrich® HEPES Sodium Salt Physical Properties
Synonyms 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt
N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt		 hepes sodium salt structure
Molecular Formula C8H17N2O4SNa MDL Number MFCD00036463 Useful pH range 6.8 - 8.2
Molecular Weight 260.29 EG/EC Number 278-169-7 pKa (at 25) 7.5
CAS Number 75277-39-3        
Sigma-Aldrich HEPES Sodium Salt Specifications Comparison
  H7006 H3784 H8651
Grade ≥99.5% (titration) BioReagent, suitable for cell culture,
≥99.5% (titration)		 BioXtra, ≥99.5% (titration)
Purity ≥99.5% ≥99.5% ≥99.5%
Reagent Testing
  H7006 H3784 H8651
Enhanced Quality
Profile Buffers		 X X X
290NM ≤0.05 ≤0.05  
260NM   ≤0.05 <0.2
280NM   ≤0.05 <0.2
IR X X  
pH     10.0 - 12.0 (20 °C, 1 M in H2O)
Karl Fischer ≤3.0% ≤3.0%  
Insoluble Matter     <0.1%
Total Impurities      
Life Science Testing
  H7006 H3784 H8651
Endotoxin   ≤0.1 eu/mg  
SPC/TAMC ≤20 cfu/g ≤1000 cfu/g  
Cell test   X  
RNAse   X  
DNAse   X  
Nickase   X  
Protease   X  
Trace Elemental Impurities
  H7006 H3784 H8651
Ammonia(NH4)     <0.05%
Phosphorus(P)     <0.005%
Chloride(Cl)     <0.05%
Sulfate(SO4)     <0.05%
Aluminum(Al)     <0.0005%
Arsenic(As)      
Barium(Ba)      
Bismuth(Bi)      
Calcium(Ca)     <0.0005%
Cadmium(Cd)      
Cobalt(Co)      
Chromium(Cr)      
Copper(Cu)     <0.0005%
Iron(Fe)   ≤0.0005% <0.0005%
Potassium(K)     ≤0.005%
Lithium(Li)      
Magnesium(Mg)     <0.0005%
Manganese(Mn)      
Molybdenum(Mo)      
Nickel(Ni)      
Lead(Pb) ≤0.0005% <0.0005% <0.001%
Strontium(Sr)      
Zinc(Zn)     <0.0005%
 
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