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Biological Buffers

HEPES Sodium Salt

BIS-TRIS Specification Comparison Printer friendly PDF version (117 Kb)


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Sigma-Aldrich® HEPES Sodium Salt Physical Properties
Synonyms     4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt
    N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt		 hepes sodium salt structure
Molecular Formula C8H17N2O4SNa MDL Number MFCD00036463 Useful pH range 6.8 - 8.2
Molecular Weight 260.29 EG/EC Number 278-169-7 pKa (at 25) 7.5
CAS Number 75277-39-3    
Sigma-Aldrich HEPES Sodium Salt Specifications Comparison
  H3787 H7006 H3784 H8651
Grade ≥99.5% (titration) ≥99.5% (titration) BPC, cell culture tested,
≥99.5% (titration)		 SigmaUltra, ≥99.5% (titration)
Purity ≥99.5% ≥99.5% ≥99.5% ≥99.5%
Reagent Testing
  H3787 H7006 H3784 H8651
Enhanced Quality
Profile Buffers		   X X X
290NM   ≤0.05 ≤0.05  
260NM     ≤0.05 <0.2
280NM     ≤0.05 <0.2
IR     X  
pH       10.0 - 12.0 (20 °C, 1 M in H2O)
Karl Fischer ≤7% ≤3.0% ≤3.0%  
Insoluble Matter       <0.1%
Total Impurities        
Life Science Testing
  H3787 H7006 H3784 H8651
Endotoxin     ≤0.1 eu/mg  
SPC   ≤20 cfu/g ≤1000 cfu/g  
Cell test     X  
RNAse     X  
DNAse     X  
Nickase     X  
Protease     X  
Trace Elemental Impurities
  H3787 H7006 H3784 H8651
Ammonia(NH4)       <0.05%
Phosphorus(P)       <0.005%
Chloride(Cl)       <0.05%
Sulfate(SO4)       <0.05%
Aluminum(Al)       <0.0005%
Arsenic(As)        
Barium(Ba)        
Bismuth(Bi)        
Calcium(Ca)       <0.0005%
Cadmium(Cd)        
Cobalt(Co)        
Chromium(Cr)        
Copper(Cu)       <0.0005%
Iron(Fe)     ≤0.0005% <0.0005%
Potassium(K)       ≤0.005%
Lithium(Li)        
Magnesium(Mg)       <0.0005%
Manganese(Mn)        
Molybdenum(Mo)        
Nickel(Ni)        
Lead(Pb) ≤0.0005% ≤0.0005% <0.0005% <0.001%
Strontium(Sr)        
Zinc(Zn)       <0.0005%
 
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