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| Sigma-Aldrich® HEPES Physical Properties |
| Synonyms |
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) |
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| Molecular Formula |
C8H18N2O4S |
Beilstein Registry |
883043 |
pKa (at 20) |
7.55 |
| Molecular Weight |
238.30 |
MDL Number |
MFCD00006158 |
pKa (at 25) |
7.48 |
| CAS Number |
7365-45-9 |
EG/EC Number |
230-907-9 |
pKa (at 37) |
7.31 |
| Useful pH range |
6.8 - 8.2 |
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| Sigma-Aldrich HEPES Specifications Comparison |
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H3375 |
H6147 |
H4034 |
H7523 |
54459 |
54457 |
| Grade |
≥99.5% (titration) |
BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration) |
BPC, ≥99.5% (titration), cell culture tested |
BioXtra,
pH 5.0 - 6.5 (20 °C, 1 M in H2O), ≥99.5% (titration) |
BioUltra, ≥99.5% (T) |
BioUltra, for molecular biology, ≥99.5% (T) |
| Purity |
≥99.5% |
≥99.5% |
≥99.5% |
≥99.5% |
≥99.5% |
≥99.5% |
| Reagent Testing |
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H3375 |
H6147 |
H4034 |
H7523 |
54459 |
54457 |
Enhanced Quality
Profile Buffers |
X |
X |
X |
X |
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| 260NM |
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≤0.05 |
≤0.045 |
≤0.045 |
≤0.045 |
| 280NM |
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≤0.05 |
≤0.035 |
≤0.035 |
≤0.035 |
| 290NM |
≤0.05 |
≤0.05 |
≤0.05 |
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| IR |
X |
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X |
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| pH |
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5.0 - 6.5 (20 °C, 1 M in H2O) |
5.0 - 6.5 (25 °C, 1 M in H2O) |
5.0 - 6.5 (25 °C, 1 M in H2O) |
| Molarity |
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| Osmolality |
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| Karl Fischer |
≤0.1% |
≤0.1% |
≤0.1% |
≤0.1% |
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| LOD |
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≤1.0% |
≤0.5% |
≤0.5% |
| Insoluble Matter |
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filter test |
filter test |
filter test |
| Residue on Ignition |
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≤0.1% |
≤0.1% |
≤0.1% |
| Life Science Testing |
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H3375 |
H6147 |
H4034 |
H7523 |
54459 |
54457 |
| Animal Component Free |
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| Endotoxin |
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≤0.1 eu/mg |
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| SPC |
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≤100 cfu/g |
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| Cell test |
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X |
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| Sterility |
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| RNAse |
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X |
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X |
| DNAse |
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X |
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X |
| Nickase |
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X |
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| Protease |
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X |
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X |
| Phosphatase |
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X |
| Embryo |
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X |
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| Trace Elemental Impurities |
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H3375 |
H6147 |
H4034 |
H7523 |
54459 |
54457 |
| Sodium(Na) |
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≤0.005% |
≤0.0050% |
≤0.0050% |
| Chloride(Cl) |
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≤0.05% |
≤0.0050% |
≤0.0050% |
| Sulfate(SO4) |
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≤0.0200% |
≤0.0050% |
| Aluminum(Al) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Arsenic(As) |
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≤0.0001% |
≤0.00001% |
≤0.00001% |
| Barium(Ba) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Bismuth(Bi) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Calcium(Ca) |
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≤0.001% |
≤0.0010% |
≤0.0010% |
| Cadmium(Cd) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Cobalt(Co) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Chromium(Cr) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Copper(Cu) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Iron(Fe) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
≤0.0005% |
| Potassium(K) |
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≤0.005% |
≤0.0050% |
≤0.0050% |
| Lithium(Li) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Magnesium(Mg) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Manganese(Mn) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Molybdenum(Mo) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Nickel(Ni) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Lead(Pb) |
≤0.0005% |
≤0.0005% |
≤0.0005% |
≤0.0005% |
≤0.0005% |
≤0.0005% |
| Strontium(Sr) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Zinc(Zn) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
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