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Biological Buffers
MES | ||
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| Sigma-Aldrich® MES Physical Properties | |||||
| Synonyms | 2-(N-Morpholino)ethanesulfonic acid 4-Morpholineethanesulfonic acid |
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| Molecular Formula | C6H13NO4S · xH2O | CAS Number | 4432-31-9 | pKa (at 20) | 6.16 |
| Molecular Weight | 195.24 (anhydrous basis) | Useful pH range | 5.5 - 6.7 | pKa (at 25) | 6.1 |
| pKa (at 37) | 5.97 | ||||
| Sigma-Aldrich MES Specifications Comparison | ||||||
| M3671 | M8250 | M5287 | M2933 | 69890 | ||
| Grade | low moisture content, ≥99% (titration) | ≥99.5% (titration) | ≥99.5% (titration), pH 2.5 - 4.0 (20 °C, 0.5 M in H2O), SigmaUltra |
BPC, plant cell culture tested, ≥99.5% | BioUltra, ≥99.5% (T) |
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| Purity | ≥99% | ≥99.5% | ≥99.5% | ≥99.5% | ≥99.5% | |
| Reagent Testing | ||||||
| M3671 | M8250 | M5287 | M2933 | 69890 | ||
| Enhanced Quality Profile Buffers |
X | X | X | X | ||
| 260NM | ≤0.025 | ≤0.025 | ||||
| 280NM | ≤0.020 | ≤0.020 | ||||
| 290NM | ≤0.05 | ≤0.05 | ≤0.05 | |||
| IR | X | X | ||||
| pH | 2.5 - 4.0 (20 °C, 0.5 M in H2O) | 2.5 - 4.0 (20 °C, 0.5 M in H2O) |
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| Karl Fischer | ≤1.0% | ≤9% | report result | ≤9% | ||
| Insoluble Matter | filter test | filter test | ||||
| Residue on Ignition | ≤0.05% | ≤0.05% | ||||
| Life Science Testing | ||||||
| M3671 | M8250 | M5287 | M2933 | 69890 | ||
| Synthetic | X | |||||
| Endotoxin | ≤0.1 eu/mg | |||||
| SPC | ≤100 cfu/g | |||||
| Plant cell test | X | |||||
| RNAse | X | |||||
| DNAse | X | |||||
| Nickase | X | |||||
| Protease | X | |||||
| Trace Elemental Impurities | ||||||
| M3671 | M8250 | M5287 | M2933 | 69890 | ||
| Sodium(Na) | ≤0.005% | ≤0.0050% | ||||
| Chloride(Cl) | ≤0.005% | ≤0.0050% | ||||
| Sulfate(SO4) | ≤0.0050% | |||||
| Aluminum(Al) | ≤0.0005% | ≤0.0005% | ||||
| Arsenic(As) | ≤0.0001% | ≤0.00001% | ||||
| Barium(Ba) | ≤0.0005% | ≤0.0005% | ||||
| Bismuth(Bi) | ≤0.0005% | ≤0.0005% | ||||
| Calcium(Ca) | ≤0.002% | ≤0.0020% | ||||
| Cadmium(Cd) | ≤0.0005% | ≤0.0005% | ||||
| Cobalt(Co) | ≤0.0005% | ≤0.0005% | ||||
| Chromium(Cr) | ≤0.0005% | ≤0.0005% | ||||
| Copper(Cu) | ≤0.0005% | ≤0.0005% | ||||
| Iron(Fe) | ≤0.0005% | ≤0.0005% | ≤0.0005% | |||
| Potassium(K) | ≤0.005% | ≤0.0050% | ||||
| Lithium(Li) | ≤0.0005% | ≤0.0005% | ||||
| Magnesium(Mg) | ≤0.0005% | ≤0.0005% | ||||
| Manganese(Mn) | ≤0.0005% | ≤0.0005% | ||||
| Molybdenum(Mo) | ≤0.0005% | ≤0.0005% | ||||
| Nickel(Ni) | ≤0.0005% | ≤0.0005% | ||||
| Lead(Pb) | ≤0.0005% | ≤0.0005% | ≤0.0005% | ≤0.0005% | ≤0.0005% | |
| Strontium(Sr) | ≤0.0005% | ≤0.0005% | ||||
| Zinc(Zn) | ≤0.0005% | ≤0.0005% | ||||
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