Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

Biological Buffers

TES

Synonym: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]ethanesulfonic acid
 

Because the pKa is 7.4 (physiological pH), TES has potential for a variety of biological applications. In many culture media that require metal cations, many buffers cannot be used due to chelation or precipitation (citrate or phosphate, for example). TES was found to be beneficial in a study of succinate oxidation1. TES is a suitable buffer for epidermal cell growth at pH 7.4 - 7.9 where additional buffering capacity is required2.
Useful pH Range 6.8 - 8.2
Working concentration range 50mM-560mM6

 

        Comparison of products by grade or quality level
Name CAS Molecular Formula Mol Wt Reagent Grade Life Science Grades PharmaGrade Solution
TES 7365-44-8 C6H15NO6S 229.25 T1375 T5641 5.05147 T6541 93359    
TES sodium salt 70331-82-7 C6H14NNaO6S 251.23 T0772       
TES hemisodium salt   C6H14.5NO6S • 0.5Na 240.24 T1030      

 

Interaction Comments

TES can serve as a stabilizer for the globular protein BSA, in aqueous solutions3
Strong interaction with Cr and Fe; weak interaction with Co, Ni, Cu and Zn

 

References

  1. Good, N.E., et al., Biochemistry, v. 5, 467 (1966).
  2. Hammar, H. and Halprin, K., Epidermis Dis., Proc. Eur. Soc. Dermatol. Res. Symp., 1979, 243 (1981).
  3. Gupta, Bhupender S.; Taha, Mohamed; Lee, Ming-Jer. 2013. Process Biochemistry. 48.11: 1686-1696.
  4. Sigma Aldrich "Product Information Sheet," http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/c5138pis.pdf
  5. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003
  6. Bartlett JMS, Stirling D, Methods in molecular biology 2nd edition Humana Press, 2003