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Biological Buffers

Tricine

Synonym: N-[Tris(hydroxymethyl)methyl]glycine
 

Tricine is a zwitterionic amino acid. It is an organic derivative of tris and glycine used in buffer solutions. Tricine has been recommended as the buffer of choice in SDS-PAGE systems, in separating proteins in the range of 1 to 100 kDa1. Tricine is also an effective hydroxyl radical scavenger used to protect molecules during radical generating procedures such as irradiation2.
Useful pH Range 7.4 - 8.8
Working concentration range 50mM3

 

        Comparison of products by grade or quality level
Name CAS Molecular Formula Mol Wt Reagent Grades Life Science Grades PharmaGrade  Solution
Tricine 5704-04-1 C6H13NO5 179.17  

 

Interaction Comments
 

Tricine strongly interacts with Magnesium, Calcium, Copper, Cobalt, Nickel and Zinc5

 

Literature References

  1. Schäger, H. and von Jagow, G., Anal. Biochem., 166, 368-379 (1987).
  2. Hicks, M. and Gebicki, J.M., FEBS Letters, 199(1), 92 (1986).
  3. Harrison MT et al, 2009 "Nitrogen in cell walls of sclerophyllous leaves accounts for little of the variation in photosynthetic nitrogen-use efficiency" Plant, Cell and Environment (32) 259-270
  4. Sorensen H et al Chromatography and Capillary Electrophoresis in Food Analysis. Royal Soceity of Chemistry, 1999.
  5. Ferreira CM, Pinto IS, Soares, EV, Soares HM. 2015 Royal Society of Chemistry 30989- 31003