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Biological Buffers
Trizma® HCl
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| Sigma-Aldrich® Trizma® HCl Physical Properties |
| Synonyms |
TRIS HCl
TRIS hydrochloride
Tris(hydroxymethyl)aminomethane hydrochloride |
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| Molecular Formula |
NH2C(CH2OH)3 · HCl |
Beilstein Registry |
3675235 |
Useful pH Range |
7.0 - 9.0 |
| Molecular Weight |
157.60 |
MDL Number |
MFCD00012590 |
pKa (at 25) |
8.1 |
| CAS Number |
1185-53-1 |
EG/EC Number |
214-684-5 |
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| Sigma-Aldrich Trizma HCl Specifications Comparison |
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T3253 |
T5941 |
T6666 |
93287 |
93363 |
| Grade |
reagent grade,
≥99.0% (redox titration), crytalline |
for molecular biology, BPC, cell culture tested,
≥99.0% (titration) |
BioXtra, pH 3.5 - 5.0 (20 °C, 0.5 M in H2O), ≥99.0% (titration) |
BioUltra, for luminescence, ≥99.0% (AT) |
BioUltra, for molecular biology,
≥99.0% (AT) |
| Purity |
≥99.0% |
≥99.0% |
≥99.0% |
≥99.0% |
≥99.0% |
| Reagent Testing |
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T3253 |
T5941 |
T6666 |
93287 |
93363 |
Enhanced Quality
Profile Buffers |
X |
X |
X |
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| λex |
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200 - 600 nm |
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| λem |
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300 - 800 nm |
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| 260NM |
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≤0.06 |
≤0.015 |
≤0.12 |
≤0.12 |
| 280NM |
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≤0.06 |
≤0.010 |
≤0.08 |
≤0.08 |
| 290NM |
≤0.05 |
≤0.05 |
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| IR |
X |
X |
X |
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| pH |
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3.5 - 5.0 (25 °C,
0.5 M in H2O) |
2.5 - 4.0 (25 °C, 4 M in H2O) |
2.5 - 4.0 (25 °C, 4 M in H2O) |
| Karl Fischer |
≤0.5% |
≤0.5% |
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| LOD |
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≤0.2% |
≤0.2% |
≤0.2% |
| Insoluble Matter |
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filter test |
filter test |
filter test |
| Residue on Ignition |
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≤0.2% |
≤0.2% |
≤0.2% |
| Life Science Testing |
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T3253 |
T5941 |
T6666 |
93287 |
93363 |
| Endotoxin |
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≤0.1 eu/mg |
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| SPC |
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≤100 cfu/g |
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| Cell test |
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X |
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| RNAse |
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X |
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X |
| DNAse |
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X |
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X |
| Nickase |
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X |
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| Protease |
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X |
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X |
| Phosphatase |
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X |
| Suitability Electrophoresis |
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X |
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| Trace Elemental Impurities |
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T3253 |
T5941 |
T6666 |
93287 |
93363 |
| Sodium(Na) |
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≤0.005% |
≤0.0050% |
≤0.0050% |
| Sulfate(SO4) |
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≤0.005% |
≤0.0050% |
≤0.0050% |
| Aluminum(Al) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Arsenic(As) |
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≤0.0001% |
≤0.00001% |
≤0.00001% |
| Barium(Ba) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Bismuth(Bi) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Calcium(Ca) |
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≤0.001% |
≤0.0010% |
≤0.0010% |
| Cadmium(Cd) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Cobalt(Co) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Chromium(Cr) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Copper(Cu) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Iron(Fe) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Potassium(K) |
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≤0.005% |
≤0.0050% |
≤0.0050% |
| Lithium(Li) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Magnesium(Mg) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Manganese(Mn) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Molybdenum(Mo) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Nickel(Ni) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Lead(Pb) |
≤0.0005% |
≤0.0005% |
≤0.0005% |
≤0.0005% |
≤0.0005% |
| Strontium(Sr) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
| Zinc(Zn) |
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≤0.0005% |
≤0.0005% |
≤0.0005% |
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