Biological Buffers

Trizma® HCl

 

Sigma-Aldrich® Trizma® HCl Physical Properties
Synonyms TRIS HCl
TRIS hydrochloride
Tris(hydroxymethyl)aminomethane hydrochloride
Trizma® HCl Image
Molecular Formula NH2C(CH2OH)3 · HCl Beilstein Registry 3675235 Useful pH Range 7.0 - 9.0
Molecular Weight 157.60 MDL Number MFCD00012590 pKa (at 25) 8.1
CAS Number 1185-53-1 EG/EC Number 214-684-5    
Sigma-Aldrich Trizma HCl Specifications Comparison
  T3253 T5941 T6666 93287 93363
Grade reagent grade,
≥99.0% (redox titration), crytalline
for molecular biology, BPC, cell culture tested,
≥99.0% (titration)
BioXtra, pH 3.5 - 5.0 (20 °C, 0.5 M in H2O), ≥99.0% (titration) BioUltra, for luminescence, ≥99.0% (AT) BioUltra, for molecular biology,
≥99.0% (AT)
Purity ≥99.0% ≥99.0% ≥99.0% ≥99.0% ≥99.0%
Reagent Testing
  T3253 T5941 T6666 93287 93363
Enhanced Quality
Profile Buffers
X X X    
λex       200 - 600 nm  
λem       300 - 800 nm  
260NM   ≤0.06 ≤0.015 ≤0.12 ≤0.12
280NM   ≤0.06 ≤0.010 ≤0.08 ≤0.08
290NM ≤0.05 ≤0.05      
IR X X X    
pH     3.5 - 5.0 (25 °C,
0.5 M in H2O)
2.5 - 4.0 (25 °C, 4 M in H2O) 2.5 - 4.0 (25 °C, 4 M in H2O)
Karl Fischer ≤0.5% ≤0.5%      
LOD     ≤0.2% ≤0.2% ≤0.2%
Insoluble Matter     filter test filter test filter test
Residue on Ignition     ≤0.2% ≤0.2% ≤0.2%
Life Science Testing
  T3253 T5941 T6666 93287 93363
Endotoxin   ≤0.1 eu/mg      
SPC   ≤100 cfu/g      
Cell test   X      
RNAse   X     X
DNAse   X     X
Nickase   X      
Protease   X     X
Phosphatase         X
Suitability Electrophoresis   X      
Trace Elemental Impurities
  T3253 T5941 T6666 93287 93363
Sodium(Na)     ≤0.005% ≤0.0050% ≤0.0050%
Sulfate(SO4)     ≤0.005% ≤0.0050% ≤0.0050%
Aluminum(Al)     ≤0.0005% ≤0.0005% ≤0.0005%
Arsenic(As)     ≤0.0001% ≤0.00001% ≤0.00001%
Barium(Ba)     ≤0.0005% ≤0.0005% ≤0.0005%
Bismuth(Bi)     ≤0.0005% ≤0.0005% ≤0.0005%
Calcium(Ca)     ≤0.001% ≤0.0010% ≤0.0010%
Cadmium(Cd)     ≤0.0005% ≤0.0005% ≤0.0005%
Cobalt(Co)     ≤0.0005% ≤0.0005% ≤0.0005%
Chromium(Cr)     ≤0.0005% ≤0.0005% ≤0.0005%
Copper(Cu)     ≤0.0005% ≤0.0005% ≤0.0005%
Iron(Fe)     ≤0.0005% ≤0.0005% ≤0.0005%
Potassium(K)     ≤0.005% ≤0.0050% ≤0.0050%
Lithium(Li)     ≤0.0005% ≤0.0005% ≤0.0005%
Magnesium(Mg)     ≤0.0005% ≤0.0005% ≤0.0005%
Manganese(Mn)     ≤0.0005% ≤0.0005% ≤0.0005%
Molybdenum(Mo)     ≤0.0005% ≤0.0005% ≤0.0005%
Nickel(Ni)     ≤0.0005% ≤0.0005% ≤0.0005%
Lead(Pb) ≤0.0005% ≤0.0005% ≤0.0005% ≤0.0005% ≤0.0005%
Strontium(Sr)     ≤0.0005% ≤0.0005% ≤0.0005%
Zinc(Zn)     ≤0.0005% ≤0.0005% ≤0.0005%