Biological Buffers

Trizma® Base (Tris Base)

Trizma Specification Comparison Printer friendly PDF version (440 kb) FAQs and Live Chat Banner Image

 

Sigma-Aldrich Trizma Base Physical Properties
Synonyms 2-Amino-2-(hydroxymethyl)-1,3-propanediol
THAM
Tris base
Tris(hydroxymethyl)aminomethane
Trometamol
Molecular Formula NH2C(CH2OH)3 Beilstein Registry 741883 Useful pH range 7.0-9.0
Molecular Weight 121.14 MDL Number MFCD00004679 pKa ( at 20)
 8.20
CAS Number 77-86-1 EG/EC Number 201-064-4 pKa (at 25) 8.06
Melting point 168-172 °C Boiling point 219-220 °C/10 mmHg pKa (at 37) 7.72
Sigma-Aldrich Trizma Base Specifications Comparison
  T1378 252859 T1503 T6687 T6791 93350 T6066 93362
Grade Crystalline ACS-reagent Primary Standard
and Buffer,
Crystalline		 Meets USP testing BioXtra Analytical, Puriss P.A. BPC, Cell Culture, EP, USP, USP/NF BioChemika, BioUltra, Molecular Biology
Purity ≥99% ≥99.8% ≥99.9% ≥99.0% ≥99.9% ≥99.7% ≥99.9% ≥99.8%
Reagent Testing
  T1378 252859 T1503 T6687 T6791 93350 T6066 93362
    X   X   X  
230nm           ≤0.6    
260nm         ≤ 0.025 ≤0.05 ≤0.06 ≤0.10
280nm         ≤ 0.020 ≤0.04 ≤ 0.06 ≤0.08
290nm ≤0.4 ≤0.2 ≤0.05       ≤ 0.05  
430nm           ≤0.01    
IR   X            
Identity       X        
Identity A             X  
Identity B             X  
Identity C             X  
Identity EP             X  
Identity USP             X  
Karl Fischer ≤0.5% ≤2% ≤0.2%       ≤0.2%  
LOD       ≤1.0% ≤0.5%   ≤0.5% ≤0.5%
Insoluble Matter   ≤0.005%     ≤0.005%     Filter test
Residue on Ignition       ≤0.1% ≤0.01% ≤0.01% ≤0.1% ≤0.01%
Life Science Testing
  T1378 252859 T1503 T6687 T6791 93350 T6066 93362
Endotoxin             ≤1 eu/mg  
SPC             ≤100 cfu/g  
Cell test             x  
RNAse             x x
DNAse             x x
Nickase             x  
Protease             x x
Phosphatase               x
Suitability Electrophoresis             x  
Trace Elemental Analysis
  T1378 252859 T1503 T6687 T6791 93350 T6066 93362
Sodium(Na)         ≤0.0050% ≤0.0050%   ≤0.0050%
Chloride(Cl)         ≤0.0050% ≤0.0005%   ≤0.0020%
Sulfate(SO4)         ≤0.0050% ≤0.0005%   ≤0.0005%
Aluminum(Al)         ≤0.0005%     ≤0.0005%
Arsenic(As)         ≤0.00001%     ≤0.00001%
Barium(Ba)         ≤0.0005%     ≤0.0005%
Bismuth(Bi)         ≤0.0005%     ≤0.0005%
Calcium(Ca)         ≤0.0010% ≤0.0010%   ≤0.0010%
Cadmium(Cd)         ≤0.0005% ≤0.0005%   ≤0.0005%
Cobalt(Co)         ≤0.0005% ≤0.0005%   ≤0.0005%
Chromium(Cr)         ≤0.0005% ≤0.0005%   ≤0.0005%
Copper(Cu)         ≤0.0005% ≤0.0005%   ≤0.0005%
Iron(Fe)   ≤0.0005%     ≤0.0005% ≤0.0005% ≤0.0001% ≤0.0005%
Potassium(K)         ≤0.0050% ≤0.0050%   ≤0.0050%
Lithium(Li)         ≤0.0005%     ≤0.0005%
Magnesium(Mg)         ≤0.0005% ≤0.0005%   ≤0.0005%
Manganese(Mn)         ≤0.0005% ≤0.0005%   ≤0.0005%
Molybdenum(Mo)         ≤0.0005%     ≤0.0005%
Nickel(Ni)         ≤0.0005% ≤0.0005%   ≤0.0005%
Lead(Pb) ≤0.0005% ≤0.0005% ≤0.0002%   ≤0.0005% ≤0.0005% ≤0.0002% ≤0.0005%
Strontium(Sr)         ≤0.0005%     ≤0.0005%
Zinc(Zn)         ≤0.00005% ≤ 0.0005%   ≤ 0.0005%
Related substances             x  
Heavy Metals       ≤0.001%        
Chlorides             ≤0.0100%  
 
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