Agarose Selection Guide

General Agarose Selection Guide
Specialty Agarose Selection Guide


General Agarose Selection Guide
Product Number	Application	Gel point °C	Gel strength g/cm²	Melting temperature °C	EEO	Sulfate %	Comments
Molecular Biology
A9539	Routine nucleic acid separation	36±1.5	≥1200		0.09-0.13	≤0.15	For routine nucleic acid analysis by electrophoresis or Southern or Northern blotting.
A5093	Routine nucleic acid separation	36±1.5	≥1200		0.09-0.13	≤0.15	Low background when staiing with SYBR Green or ethidium bromide
A9414	Low melting point	26-30	≥200	≤65	≤0.10	≤0.10	For DNA recovery from low melting gels
A4718	High resolution	≤35	≥300	≤75	≤0.05		For separation of small DNA fragments that differ in size by 2%.
A7431	Wide range/standard 3:1	32.5-38	≥1400	≤90	≤0.13	≤0.15	Separates DNA fragments 10-1500 bp; gels less fragile compared to A 2790
A2790	Wide range	≤35	≥500	≤65	≤0.15	≤0.15	Separate low bp DNA, 50-1000
A2929	Pulse field running gel	42±1	≥2400		0.04-0.07	≤0.08	Separation of large DNA (10-2000 KB) by pulsed-field electrophoresis
A3054	Pulse field sample gel	≤30	≥400	≤70	≤0.10	≤0.15	For preparation of chromosomal DNA samples for pulsed-field electrophoresis
Protein Electrophoresis
A4679	Immunoelctrophoresis	36±1.5	≥1200	88	0.09-0.13		For immunodiffusion and immunoelectrophoresis
A9311	Protein electrophoresis	36±1.5	≥1000	80	0.16-0.19	≤0.20
A5304	Protein electrophoresis	36±1.5	≥700	≤90	0.3	≤0.30
Cell Culture
A9045	General cell culture and insect cell culture	<30	≥200	>60	≤0.1	≤0.10	Clear gels, useful for media with heat labile components
A6560	Plant cell culture	<30	≥200	>60	≤0.1	≤0.10	Clear gels, useful for media with heat labile components

Specialty Agarose Selection Guide
Product Number	Type	Sulfate (%)	Gel Strength		Gel Point		Melting Temp. (°C)	Electroendosmosis (EEO)
			g/cm2	at %	°C	at %
A0169	I-A	<0.20	>1200 >2500	1 1.5	36 ± 1.5	1.5	87 ± 1.5	0.09-0.13
A0576	I-B	≤0.12	≥1800 ≥3200	1 1.5	36 ± 1.5	1.5	86 ± 2.0	≤0.12
A6013	I	≤0.15	≥1200	1	36 ± 1.5	1.5		0.09-0.13
A9918	II-A	<0.25	>1000 >2000	1 1.5	36 ± 1.5	1.5	87 ± 1.5	0.16-0.19
A6877	II	≤0.20	≥1000	1	36 ± 1.5	1.5		0.16-0.19
A9793	III-A	<0.25	>750 >1000	1 1.5	36 ± 1.5	1.5	87 ± 1.5	0.23-0.26
A6138	III	≤0.20	≥650	1	36 ± 1.5	1.5		0.23-0.26
A3643	IV	≤0.25	≥650	1	36 ± 1.5	1.5		≥0.30
A3768	V	≤0.30	≥800	1	42 ± 1.5	1.5		0.10
A7174	VI-A	≤0.20	>900 >1200	1 1.5	41 ± 1.5	1.5	95 ± 1.5	≤0.14
A3893	VI	≤0.20	≥1000	1.5	42 ± 1.5	1.5		0.1
A0701	VII-A	≤0.10	≥250 ≥500	1 1.5	26 ± 2	1.5	≤65.5	≤0.12
A4018	VII	≤0.10	≥200	1	26-30	1.5	≤65	≤0.1
A4905	VIII	≤0.20	≥500	1	ND			±0.02
A2576	IX-A	≤0.14	≥100 ≥400	1 1.5	≤17	1.5	≤60	≤0.11
A5030	IX	≤0.10	≥75	2	8-17	0.8	≤50	≤0.05
A3038	XI	≤0.15	≥500	4	≤35	4	≤65	≤0.15
A7299	XII	≤0.20	≤500 ≤900	1 1.5	41 ± 1.5	1.5	95 ± 1.5	≤0.14

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