Core Bioreagents

Agarose Selection Guide

General Agarose Selection Guide

 
Product No. Application Gel point
°C
Gel strength g/cm2 Melting temperature °C EEO Sulfate % Comments
Molecular Biology
A9539 Routine nucleic acid separation 36±1.5 ≥1200   0.09-0.13 ≤0.15 For routine nucleic acid analysis by electrophoresis or Southern or Northern blotting.
A9414 Low melting point 26-30 ≥200 ≤65 ≤0.10 ≤0.10 For DNA recovery from low melting gels
A4718 High resolution ≤35 ≥300 ≤75 ≤0.05   For separation of small DNA fragments that differ in size by 2%.
A7431 Wide range/standard 3:1 32.5-38 ≥1400 ≤90 ≤0.13 ≤0.15 Separates DNA fragments 10-1500 bp; gels less fragile compared to A 2790
A2790 Wide range ≤35 ≥500 ≤65 ≤0.15 ≤0.15 Separate low bp DNA, 50-1000
A2929 Pulse field running gel 42±1 ≥2400   0.04-0.07 ≤0.08 Separation of large DNA (10-2000 KB) by pulsed-field electrophoresis
A3054 Pulse field sample gel ≤30 ≥400 ≤70 ≤0.10 ≤0.15 For preparation of chromosomal DNA samples for pulsed-field electrophoresis
05066 Molecular biology 35±1.5 1500
0.23-0.27 <0.6 % Nucleic Acid Separation
Protein Electrophoresis
A4679 Immunoelctrophoresis 36±1.5 ≥1200 88 0.09-0.13   For immunodiffusion and immunoelectrophoresis
A5304 Protein electrophoresis 36±1.5 ≥700 ≤90 0.3 ≤0.30  
Cell Culture
A9045 General cell culture and insect cell culture <30 ≥200 >60 ≤0.1 ≤0.10 Clear gels, useful for media with heat labile components

 

Specialty Agarose Selection Guide

 
    g/cm2 at % °C at %    
Product Number Sulfate (%) Gel Strength Gel Point Melting Temp. (°C) Electroendosmosis (EEO)
A0169 <0.20 >1200
>2500
1
1.5
36 ± 1.5 1.5 87 ± 1.5 0.09-0.13
A0576 ≤0.12 ≥1800
≥3200
1
1.5
36 ± 1.5 1.5 86 ± 2.0 ≤0.12
A6013 ≤0.15 ≥1200 1 36 ± 1.5 1.5   0.09-0.13
A9918 <0.25 >1000
>2000
1
1.5
36 ± 1.5 1.5 87 ± 1.5 0.16-0.19
A6877 ≤0.20 ≥1000 1 36 ± 1.5 1.5   0.16-0.19
A9793 <0.25 >750
>1000
1
1.5
36 ± 1.5 1.5 87 ± 1.5 0.23-0.26
A3643 ≤0.25 ≥650 1 36 ± 1.5 1.5   ≥0.30
A7174 ≤0.20 >900
>1200
1
1.5
41 ± 1.5 1.5 95 ± 1.5 ≤0.14
A0701 ≤0.10 ≥250
≥500
1
1.5
26 ± 2 1.5 ≤65.5 ≤0.12
A4018 ≤0.10 ≥200 1 26-30 1.5 ≤65 ≤0.1
A2576 ≤0.14 ≥100
≥400
1
1.5
≤17 1.5 ≤60 ≤0.11
A5030 ≤0.10 ≥75 2 8-17 0.8 ≤50 ≤0.05